Uvitec Alliance Q4-365 Advanced

Full Imaging Platform

Overview

The UVITEC Alliance Q4 is an advanced imaging system based on proprietary optics and a super-cooled 9.2 megapixel, 16-bit pixel depth camera. The large 1 inch SONY CCD sensor and extremely bright motorised fixed lens (f0.90) provide low noise, increased sensitivity and resolution and make the Alliance Q4 ideal for visible light, fluorescent, chemiluminescent (Western blot), chemifluorescent, biofluorescent and multiplexed imaging applications. The Alliance Q9 incorporates an impressive list of features such as auto-focus, auto-exposure, UV security timer, motorized 6 position filter wheel and multiple wavelength illumination options.

The Alliance Q4 incorporates the NET LED lighting module (please see below). The NET LED is an epi-illumination module into which powerful LED red, green, blue, NIR or infrared strip lights can be inserted. The NET LED modules are attached on each side of the Alliance Q4 darkroom with adjustable arms and the long-life LEDs are organized in arrays in order to maximize excitation energy on the sample.

The NET LED module allows the automatic setting of filters, lens focus and exposure settings to ensure data consistency and ease of use and its incorporation into the Alliance Q4 expands the capabilities of the system to include multiplexed fluorescence and chemifluorescence. The module, when fitted with infrared arrays, can be used to image LI-COR and similar infrared dyes.

The UVITEC Alliance Q9-365 Advanced 365 also features either 254 or 365 nm epi-UV illumination to expand its applications.

Uvitec Alliance Q4 Advanced product video.

Uvitec Alliance Q4 Advanced Details



Camera & Optics
4-megapixel native resolution Latest generation CCD sensor, QE peak larger than 73% 16-bit camera, 65,535 shades of grey
-71°C regulated, 4-stage Peltier cooling Panoramic lens (25mm), super-wide F/0.90 4.8 OD dynamic range
24cm, closest sample-to-camera distance Extra-wide 24x20cm field of view .

Darkroom
tainless-steel made for extreme robustness Long-lasting chemical-resistant Epoxy paint Full door aperture for easiest darkroom access Fully slide-out, plug-and-play transilluminator Fully-motorised 6+1-position wheel, F-590 included UviPure© patented technology available 100% computer-controlled darkroom One-click© image acquisition process

Illuminations
Included:
• Epi-white LED panels
• Chemiluminescence-ready
• Large choice of plug-&-play transilluminators:
    • 302nm, 21x26cm
    • 26MX – 302nm, Uvipure©, 21x26cm
    • 26LM – 302+365nm, 21x26cm
    • 26LMX – 302+365nm, Uvipure©, 21x26cm
    • 20-White or 20-Blue – 20x20cm
• BioPanel© RGB LED panels

Optional:
• Upgrade to ChromaPure© net-LED (customisable)
• Conversion screen (white or blue)

Chemical attraction
• Chemiluminescence, bio-luminescence, fluorescence and visible
• Multiplexing and bio fluorescence ready
• Quantification and documentation
• Gene expression, protein expression; RNA/DNA assay, colonies
• In vivo by luciferase
• RGB Led equipped
• Optional in vivo by RFP/GFP
• Optional “Chromapure” module to image any blot conjugated with any dyes available on the market from Invitrogen,
GE Life Science, Thermo Pierce, Sigma, Millipore, Promega, Licor
• Optional customisable multiplexing

Epi red, green and blue “Chromapure” module technology
The Alliance Q4 imaging system can be fitted with an epi red, green and blue “Chromapure” module for multiplexing. This module uses unique NET LED epi fluorescence technology to give a stronger and more even light across the entire sample area. This contrasts to the spot LEDs in other gel imaging systems that emit a weaker signal and create non-uniform lighting across the sample area.

What also differentiates UVITEC's NET LED technology from other epi fluorescence systems is that the “Chromapure” module has three individual high end filters placed over the NET LED module. These filters only allow the excitation peak of the red, green or blue LED light to pass through to maximally excite your fluorophores.

Using peak excitation reduces the degree of signal overlap during multiplexing and the LED NET module ensures that this strong excitation signal is spread evenly across the sample area. Eight different emission filters are available for the NET LED module so that you can choose the correct filter to match your fluorescent dye's emission spectra.

In the following table you will find a list fluorescence dyes which are compatible with the “Chromapure” module:

Blue excitation Green excitation Red/Near Infra-red excitation Infra-red excitation
SYBR Safe® ALEXA FLUOR 532® ALEXA FLUOR 635® DYLIGHT 820®
SYBR Green I® ALEXA FLUOR 546® CY5, CY5.5® DYLIGHT 800®
SYBR Green II® CY3® DYLIGHT 649® IR DYE 800®
SYBR Gold® DYLIGHT 549® BODIPY 650®
FITC® SYPRO RED ® ALEXA FLUOR 647®
EGFP®, GFP® DEEP PURPLE ® CY7®
CY2® RHODAMINE RED® DYLIGHT 690®
FAM® LI680/IRDye 680®
ALEXA FLUOR 488® ALEXA FLUOR 790®
DYLIGHT488® ALEXA FLUOR 750®
SYPRO Red®
GEL GREEN®
ATTOPHOS®


uvitec images

• Scientific grade CCD camera
• Super HD 4 megapixel native camera resolution
• Chemiluminescence , bio-luminescence, epi white light LED and UV excitation
• RGB LED included
• Optional IR/NIR customisable “Chromapure” module
• Optional customisable multiplexing
• Automatic lighting controls
• Custom emission filters available
• Motorised multi position filter wheel
• 16-bit depth camera (65,535 shades of gray)
• Dynamic range: 4.8 OD
• Extreme sensitivity and resolution
• Electronically variable shutter speed
• Ideal for sensitivity demanding applications such as Western blots or in vivo luciferase
• Ideal for resolution demanding applications such as 1D quantification, 2D gels
• Cooling -60°C regulated 3 stage Peltier for the lowest noise and the best detection of the weakest band
• Extremely bright custom made motorised fixed lens (F0.90)
• Autofocus, auto-exposure
• Robust steel and stainless steel darkroom construction, epoxy painted chemical resistant
• Roll-out transilluminator, UVI pure patented option
• Advance UVIband software available
• Direct access to key functions
• Multi-user capability
• Good Laboratory Practice (GLP) file
• IQ/PQ/OQ available
• “One touch” fully automated image acquisition program

Specifications
• Resolution: 4 megapixels native resolution
• Quantum efficiency (QE) peak:larger than 73%
• Lens: 24 mm
• Lens aperture: f0.90
• Distance to sample: 24 cm
• Field of view: 24 cm x 20 cm
• Pixel depth: 16 bit
• Grey scales: 65536
• Dynamic range: 4.8 OD
• Filter wheel: 7 position motorised
• Cooling: -80°C compared to ambient

System configuration
The following items are included in the purchase of the Alliance Q4:
• Darkroom with epi white light
• 4 megapixel CCD camera with f0.90 lens
• 6+1 position motorised filter wheel
• UVIPure transilluminator

• Unlimited free license for the NINE-ALLIANCE image acquisition and analysis software
• Emission filter (F590) for use with Ethidium bromide and other fluorophores that emit in the 590 nm range

• Chemiluminescence, Bioluminescence
• UV Fluorescence
• RGB LED excitation
• Fluorescence on Western Blots: Optional IR/NIR “Chromapure” module
• Optional Customisable Multiplexing

Alliance Q4 Advanced NineAlliance Software

A wide range of software functions are incorporated into the UVI 1D image acquisition and analysis software that is supplied with the Alliance Q9. These include intuitive menus, buttons that are easy to understand and to operate and a wide range of automatic settings and protocols to quicken and simplify the image acquisition process. If you prefer a more hands-on approach then plenty of manual functionality has also been incorporated into the Alliance Q9's software. Key features of the software include:

Acquisition
• Auto - Manual - Serial
• Optimal exposure time Live-3D acquisition mode
• tiff-delivered pictures

Customise
• Create your own acquisition protocols
• Create templates
• Set your parameters

Visualisation
• Adjust Gamma & SoG
• Visualise all results in 3D
• Full resolution images

Editing
• Improve image display
• Add text, date, name and save templates
• Change image dynamic

Analysis
• Quantification & normalisation
• Molecular Weight
• Export data to Excel

Unlimited free license for the NINE-ALLIANCE image acquisition and analysis software