BioTek Cytation 1

Cell Imaging Multi-Mode Reader

Overview

Cytation™ 1 Cell Imaging Multi-Mode Reader combines fluorescence and high contrast brightfield imaging with conventional multi-mode detection without the expense and complexity commonly associated with other digital microscopy systems. This unique, patented design provides both quantitative phenotypic cellular information with well-based quantitative data. Cytation 1's multi-mode detection module includes high sensitivity filter-based fluorescence and a monochromator system for UV-Vis absorbance. Temperature control and shaking are standard; CO2/O2 control and reagent injectors are available. BioTek’s powerful Gen5 software makes image capture and plate reading easy.

• Affordable, patented, quantitative digital microscopy and optional multi-mode microplate detection
• Augmented Microscopy™ using Gen5 software for seamless image capture to quantitative publication ready data with no extensive training required
• Fluorescence and high contrast brightfield imaging from 1.25x to 60x to capture large regions of interest to 60x or intracellular details
• Affordable automation: automated XY stage, focus, exposure, image capture and LED intensity
• Cell friendly design - 4-Zone incubation to 45°C with Condensation Control, CO2/O2 control, angled injectors and lidded plate handling
• High performance filter-based fluorescence detection with convenience of monochromator based UV-Vis absorbance

Cytation 1 product video.

BioTek Cytation 1 Details



Collect both phenotypic data and quantitative data from one instrument. Image and data analysis are provided with Gen5 software for all applications

Patented Hybrid optics: sensitivity of filters, flexibility of monochromator
Cytation 5’s hybrid optical design offers filter-based fluorescence optics for high transmission and high sensitivity, while the available monochromator optics offers convenience and assay flexibility. Multiple fluorescence measurement modes include fluorescence intensity, time-resolved fluorescence and fluorescence polarization

Augmented Microscopy™ using Gen5 software: image capture to quantitative publication ready data.
Gen5 software takes you automatically through the steps typically performed in microscopy: capture > process > analyze > publish. The interface is logical and user-friendly, so your imaging workflows can be up and running without extensive training.

Fluorescence and high contrast brightfield imaging
The powerful imaging module in Cytation 1 offers four color channels plus brightfield, from 1.25x for imaging larger samples to 60x for intracellular details. More than 15 filter/LED cubes are available, supporting a broad range of imaging applications.

Affordable automation
Cytation 1 has an automated XY stage, autofocus, auto exposure and auto LED intensity for efficient capture of high quality images. The automated features in Cytation 1 and Gen5 make it easy for you to find the region of interest, then quickly find the best settings to capture the images. Gen5 software is simple to use, without extensive training.

Cell friendly design
Live cell assays require an optimal environment. Cytation 1 has temperature control and shaking, plus CO2 / O2 control, to facilitate longer-term kinetic studies.

High performance and convenience
To optimize multi-mode detection, Cytation 1 has high performance filters for fluorescence and monochromator-based UV-Vis absorbance for convenient wavelength selection in 1 nm increments.

GENERAL
Detection modes UV-Vis absorbance
Fluorescence intensity
Luminescence
Fluorescence polarization
Time-resolved fluorescence
Read methods Endpoint, kinetic, spectral scanning, well area scanning
Microplate types Monochromator: 6- to 384-well plates
Filters: 6- to 1536-well plates
Imaging: 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer)
Take3 Micro-Volume Plates
Temperature control 4-Zone incubation to 45 °C with Condensation Control™; Variation +0.2 °C at 37 °C
Shaking Linear, orbital, double orbital
Software Gen5™ Microplate Reader and Imager Software
Gen5 Image+ and Image Prime software available for full image analysis
Gen5 Secure available for 21 CFR Part 11
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
CO2 and O2 control (option) 0 - 20% CO2 control and 1 – 19% O2 control, with optional Gas Controller
Models for both CO2 and O2 or CO2 only are available
IMAGING SYSTEM
Imaging mode Fluorescence
High Contrast Brightfield (2.5x Zeiss, 4x, 10x, 20x, 40x and 60x)
Imaging method Single color, multi-color, montage, time lapse, z-stacking
Image processing Z-projection, image stitching
Camera 16-bit gray scale, Sony CCD
Objective capacity 2 user-replaceable objectives
Objectives available 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x
Image filter cube capacity 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647
Imaging LED cubes available 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions Autofocus, auto LED intensity, auto exposure
Autofocus method Image-based autofocus
User-trained autofocus
Laser autofocus (option)
Positional controls Software control
Image collection rate Image-based autofocus: 
96 wells, 1 color (DAPI), 4x, 6 minutes
96 wells, 3 colors, 4x, 12 minutes
 
Laser autofocus:
96 wells, 1 color (DAPI), 4x, <3 minutes
96 wells, 3 colors, 4x, <7 minutes, 30 seconds Burst Mode: 10 fps, single well, single color at <= 50ms integration time
Image Analysis Software option Gen5 Image+: Image analysis
Gen5 Image Prime: Advanced image analysis
Gen5 Secure Image+: Advanced image analysis, 21 CFR Part 11 features
FLUORESCENCE INTENSITY
Light source Xenon flash
Detector PMT
Wavelength selection Deep blocking band pass filters / dichroic mirrors
Wavelength range Filters: 200 - 700 nm (850 nm option)
Dynamic range 7 decades
Sensitivity Fluorescein: 0.25 pM (0.025 fmol/well, 384-well plate)
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
LUMINESCENCE
Wavelength range 300 - 700 nm
Dynamic range >6 decades
Sensitivity 10 amol ATP (flash)
100 amol (glow)
FLUORESCENCE POLARIZATION
Light source Xenon flash
Detector PMT
Wavelength selection Filters
Wavelength range 400 nm – 700 nm
Sensitivity 1.2 mP standard deviation at 1 nM fluorescein
TIME-RESOLVED FLUORESCENCE
Light source Xenon flash
Detector PMT
Wavelength selection Filters
Sensitivity Europium 40 fM (4 amol/well, 384-well plate)
ABSORBANCE
Light source Xenon flash
Detector photodiode
Wavelength selection Monochromator
Wavelength range 200 – 999 nm, 1 nm increment
Monochromator bandwidth 2.4 nm
Dynamic range 0 – 4.0 OD
Resolution 0.0001 OD
Pathlength correction yes
Monochromator wavelength accuracy + 2 nm
Monochromator wavelength repeatability + 0.2 nm
OD accuracy <1% at 2.0 OD
<3% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
REAGENT INJECTORS (OPTION)
Supported detection modes All modes
Number 2 syringe pumps
Supported labware 6- to 384-well plates, Petri and cell culture dishes
Dead volume 1.1 mL with back flush
Dispense volume 5 - 1000 µL in 1 µL increments
Plate geometry 6- to 384-well microplates
Dispense accuracy +1 µL or 2%
Dispense precision <2% at 50 – 200 µL
PHYSICAL CHARACTERISTICS
Power 100-240 VAC, 50/60 Hz (24VDC external power supply, 150W min)
Dimensions 20" D x 16.5" W x 17.5" H
(50.8 cm x 41.91 cm x 44.5 cm)
Weight 65 lbs (29 Kg)
REGULATORY
Regulatory CE and TUV marked. Models for In Vitro Diagnostic use are available.
Take3


Micro-volume quantification is fast and easy with the Take3™ and Take3 Trio Micro-Volume plates, used in your BioTek microplate reader. Measure multiple 2 µL samples at a time, without diluting and without needing specialized equipment. Pre-programmed nucleic acid and protein protocols in Gen5 make quantification fast and easy.

  • Low volume 2 µL nucleic acid and protein quantification
  • Pre-programmed protocols in Gen5 Software
  • Absorbance and fluorescence detection
  • Easy, wipe-off maintenance
  • Read 2 µL microspots, BioCells or standard cuvettes
  • Gen5


    Gen5 Microplate Reader and Imager Software controls Lionheart FX for automated image capture, processing, analysis and development of publication-ready images and data. This imaging and microscopy automation represents BioTek’s Augmented Microscopy™ - the simplified, automated process to accomplish multiple microscopy steps in a single platform. Gen5 is available in several editions to meet the image analysis requirements across a range of applications.

    Gen5 Image+ offers complete imaging control for Lionheart FX Automated Microscope, and provides image processing and analysis including z-projection, image stitching, hitpicking and digital phase contrast.

    Gen5 Image Prime controls Lionheart FX Automated Microscope includes more expansive image processing and analysis: Z-projection, image stitching, hitpicking and digital phase contrast, plus advanced features including secondary mask, dynamic thresholding and subpopulation analysis.

    Gen5 Secure editions add features to help ensure compliance to 21 CFR Part 11 requirements. Gen5 add-on modules provide increased functionality for specific applications or analyses, such as Spot Counting.


    Configurations


    Imaging Plus Multi-Mode Configurations

    CYT1FAV CYT1FA CYT1V
    Fluorescence and high contrast brightfield imaging
     
    Monochromator absorbance
     
    Filter/dichroic fluorescence
     
    Luminescence / filtered luminescence