BioTek Cytation 7

Cell Imaging Multi-Mode Reader

Overview

Cytation™ 7 Cell Imaging Multi-Mode Reader combines automated digital upright and inverted widefield microscopy with conventional multi-mode microplate reading in a unique, modular patented design. The upright microscopy module with reflected and transmitted light imaging enables even more applications such as ELISpot, Colony counting, material inspection and fast slide scanning in combination with region of interest (ROI) detection workflow.



Configurations

In total, we offer five microscopes that are all available in multiple configurations to meet the specific criteria of your lab. The Cytation systems are the most modular and flexible imaging systems on the market, as they come as they are available as:

• Imaging Only System, upgradable for plate reader capabilities
• Multi-mode reader Only System, upgradable for imaging
• Imaging & Multi-Mode Reader System as one compact platform

• Please contact us here to discuss which type is the best fit for your laboratory.

Video


BioTek Cytation 7 Details



Imaging Module:

modules


• Fluorescence, brightfield, high contrast brightfield, color brightfield, upright reflected brightfield and transmitted color brightfiel for maximum application reach

• Automated Inverted and/or digital upright microscopy for a broad range of applications

• Label-free cell counting for cell proliferation studies

• Supported labware; 6-1536 microplates, microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides

• 4 channels and more than 20 color LED/filter cubes available for multi-color fluorescent imaging
• Automated digital microscopy with 1.25x up to 60x magnification

• Optimized conditions for long-term live cell imaging applications providing 4-zone incubation to 45°C incuding condensation control and effective containment for CO2/O2 control.

modules


• An available dual reagent injector facilitates rapid kinetic assays

• Affordable automation: automated XY stage, focus, exposure, image capture and LED intensity

• Cytation 7 and Gen5 together comprise Augmented Microscopy™: i.e., the automation of image capture, processing, analysis and development of publication-ready images and data.

• Multiple focus methods, both software and hardware based to facilitate assays of interest.

Multi-mode Plate Reader module

• Wide range of measurement modes, including fluorescence intensity, luminescence and UV-VIS.

• Variable bandwidth monochromators for unmatched versatility and performance to optimize fluorophore detection e.g.eliminating stokes shiftes. The bandwidth are selectable from 9 nm to 50 nm, in 1 nm increments.

modules


• Three types of shaking methods: linear, orbital and double orbital

In total, we offer five microscopes that are all available in multiple configurations to meet the specific criteria of your lab.

• Please contact us here to discuss which type is the best fit for your laboratory.
Features


Automated digital microscopy to 60x:
Cytation 7 brings an unprecedented level of automated microscopy, plus multi-mode detection into one platform. It includes two camera’s, an upright and inverted microscope optics which opens up a wide range of cellular and reflected light applications. The monochromator plate reader optics allows running all of the standard plate reader assays.

Cytation 7 with Augmented Microscopy automates your imaging workflow to yield amazing images and powerful data. Automated image capture starts with image-based and laser autofocus, plus auto LED and exposure. Automated image pre-processing optimizes images for downstream analysis, from cell counting to characterization of subcellular details.

Features


Expandend automation:
Many life science workflows benefit from expanded automation, for increased throughput or process efficiency. BioTek offers unique automation solutions that integrate with our microplate washers, dispensers, readers and imagers.

BioSpa 8 Automated Incubator links BioTek readers or imagers together with washers and dispensers for full workflow automation of up to 8 microplates. Temperature, CO2/O2 and humidity levels are controlled and monitored through the BioSpa software to maintain an ideal environment for cell cultures during all experimental stages.

biospa

More info: BioSpa 8 Automated Incubator


BioStack plate stacker
BioStack is a compact stacker that offers fast plate exchange options for up to 50 plates, and patented plate de-lidding and re-lidding with BioStack 4. Its rapid plate exchange speeds increase throughput and enhance productivity, accommodating assay workflows for 96- and 384-well plates.

biostack

More info: BioStack plate stacker


Modular design :
Add modules as your research requirement change over time. For instance: choose the Cytation 7 first as a microscope and upgrade it later on with Multi-mode reader capabilities or vice versa.

Two camera’s, up to six imaging modes and multiple imaging processes
• Wide field of view color upright microscope for color reflected and transmitted brigthfield

• Wide field of view monochrome inverted microscope for brightfield, high contrast brightfield, color brightfield and fluorescence imaging in four channels – and nearly twenty available filter/LED cubes, Cytation 7 offers fantastic versatility that isn’t limited to image capture. Follow your imaging processes with aid of:

- 3D imaging performed with Z-stacking,

- Montage collection and stitching

- Fast and Long-term live cell kinetics including movie making

- Cellular analysis tools e.g. cell count, object size, mean and area, subpopulation analysis

timelapse


Label-free cell counting
It’s fast and convenient to count live cells using the available high contrast brightfield option. Higher contrast allows a direct and label-free method for object identification using Gen5 software to count cells and characterize cell proliferation in kinetic studies.

ELISpot imaging
Cytation 7’s upright imaging module can be used to automate assays such as ELISpot in which cell secretions are rendered visible through the use of a colorimetric reaction. Cytation 7 fully automates imaging acquisition, processing, image analysis and object count.

elispot


Hit-picking: Multi-mode detection + imaging saves time and data storage

hitpicking


(1) Plate reader quickly identifies GFP positive wells.
(2) Only GFP positive wells are imaged, saving both time and computer memory.

Powerful functionality with ROI identification feature
Cytation 7 and Gen5 software facilitate ROI identification. Cytation 7 scans samples at low magnification before prompting the user to identify regions of interest to be imaged at high magnification. This greatly accelerates the process of imaging ROIs in batches of complex microscopic samples.

roi


Kinetic live cell assay support
Several options are available to support live cell assays with Cytation 7, including an environmental cover to maintain temperature and gas levels within the system. Long-term live cell kinetics are supported, using the unique humidity chamber that fits on the stage itself, keeping the live cell in a completely nurturing environment. A dual reagent injector provides quick inject/image capability for observing fast reactions.

Easy-to-use Software & Compact futureproof equipment
• Cytation 7 integrates all microscopy hardware and multi-mode detection into a very compact footprint, saving valuable bench space. • The easily accessible objectives and LED/filter cubes make setup and operation fast and simple, with a minimal learning curve. • The user-friendly software eliminates the need of highly trained operators. The software is convenient for all user levels.

stage


Expanding your research applications with the Cytation 7:

assay


Take3


Micro-volume quantification is fast and easy with the Take3™ and Take3 Trio Micro-Volume plates, used in your BioTek microplate reader. Measure multiple 2 µL samples at a time, without diluting and without needing specialized equipment. You can run 16 or 48 samples in one run to save a lot of time compared to single-sample devices. Gen5 is pre-programmed for ssDNA, dsDNA, RNA and protein quantification makes it fast and easy.

• Low volume 2 µL nucleic acid and protein quantification • Pre-programmed protocols in Gen5 Software • Absorbance and fluorescence detection (e.g. Picogreen) • Easy, wipe-off maintenance • Read 2 µL microspots, BioCells or standard cuvettes
GENERAL
Detection modes UV-Vis absorbance
Fluorescence intensity
Luminescence
Read methods Endpoint, kinetic, spectral scanning, well area scanning
Microplate types Monochromator: 6- to 384-well plates
Imaging: 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer)
Take3 Micro-Volume Plates
Temperature control 4-Zone incubation to 45 °C with Condensation Control; +0.5 °C at 37 °C
Cooling Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides internal cooling after incubated processes.
Shaking Linear, orbital, double orbital
Software Gen5™ Microplate Reader and Imager Software included
Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
CO2 and O2 control (option) Range: 0 - 20% (CO2); 1 - 19% (O2)
Models for both CO2 and O2 or CO2 only are available
IMAGING - INVERTED MICROSCOPE
Imaging mode Fluorescence, color brightfield, user-selectable brightfield/high contrast brightfield
Imaging method Single color, multi-color, time lapse, montage, z-stacking, z-stack montage
Image processing Z-projection, digital phase contrast, stitching
Camera 16-bit Sony CMOS wide field of view monochrome camera
Objective capacity 6-position automated turret for user-replaceable objectives
Objectives available 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x
Image filter cube capacity 4 user-replaceable fluorescence cubes, plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-FRET, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, Tag BFP-FRET, GFP (Ex)-CY5 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex 377/Em 647, Oxidized roGFP2, TRITC
Imaging LED cubes available 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 554 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions Autofocus, user-trained autofocus, autoexposure, auto-LED intensity
Autofocus method Image-based autofocus
User-trained autofocus
Laser autofocus (option)
Positional controls Software Control
Joystick controller (option)
Image collection rate Image-based autofocus:
96 wells, 1 color (DAPI), 4x, 6 minutes
 
Laser autofocus:
96 wells, 1 color (DAPI), 4x, <3 minutes
Image Analysis Software option Gen5 Image+: Image analysis
Gen5 Image Prime: Advanced image analysis
Gen5 Secure: 21 CFR Part 11 compliant features
IMAGING - UPRIGHT MICROSCOPE
Imaging modes Reflected color brightfield; transmitted color brightfield
Imaging methods Single image, montage, time lapse, Z-stacking
Image processing Z-projection, digital phase contrast, stitching
Camera 16-bit Sony CMOS wide field of view color camera
Lenses Finder scope, 2x, 4x, 8x
Positional controls Software Control
Joystick controller (option)
Image collection rate Entire 100 mm dish @ 1x: <2:40 minutes
Entire microscope slide @1x: <1:15 minutes
96-well ELISpot plate@ 1x: <5 minutes
Image Analysis Software option Gen5 Image+: Image analysis
Gen5 Image Prime: Advanced image analysis
Gen5 Secure: 21 CFR Part 11 compliant features
FLUORESCENCE INTENSITY
Light source Xenon flash
Detector PMT (red-shifted PMT option)
Wavelength selection Quad monochromators (top/bottom)
Wavelength range 250 – 700 (900 nm option)
Monochromator bandwidth Variable, from 9 nm to 50 nm in 1 nm increments
Dynamic range 7 decades
Sensitivity Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) – top
Fluorescein 4 pM (0.4 fmol/well, 384-well plate) – bottom
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
LUMINESCENCE
Wavelength range 300 - 700 nm
Dynamic range >6 decades
Sensitivity 20 amol ATP (flash)
ABSORBANCE
Light source Xenon flash
Detector Photodiode
Wavelength selection Monochromator
Wavelength range 230 - 999 nm, 1 nm increment
Monochromator bandwidth 4 nm (230 - 285 nm), 8 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction Yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
REAGENT INJECTORS (OPTION)
Number 2 syringe pumps
Supported labware 6- to 384-well plates, Petri and cell culture dishes
Dead volume 1.1 mL with back flush
Dispense volume 5 - 1000 µL in 1 µL increments
Plate geometry 6- to 384-well microplates
Dispense accuracy +1 µL or 2%
Dispense precision <2% at 50 - 200 µL
PHYSICAL CHARACTERISTICS
Power 150 Watts maximum consumption
Dimensions 20.2" D x 16.4" W x 17.5" H
(51.4 cm D x 41.6 cm W x 44.5 cm H)
Weight 80 lbs (36.3 kg)
REGULATORY
Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.
Gen5


Gen5 Microplate Reader and Imager Software controls Lionheart FX for automated image capture, processing, analysis and development of publication-ready images and data. This imaging and microscopy automation represents BioTek’s Augmented Microscopy™ - the simplified, automated process to accomplish multiple microscopy steps in a single platform. Gen5 is available in several editions to meet the image analysis requirements across a range of applications.

Gen5 Image+ offers complete imaging control for Lionheart FX Automated Microscope, and provides image processing and analysis including z-projection, image stitching, hitpicking and digital phase contrast.

Gen5 Image Prime controls Lionheart FX Automated Microscope includes more expansive image processing and analysis: Z-projection, image stitching, hitpicking and digital phase contrast, plus advanced features including secondary mask, dynamic thresholding and subpopulation analysis.

Gen5 Secure editions add features to help ensure compliance to 21 CFR Part 11 requirements. Gen5 add-on modules provide increased functionality for specific applications or analyses, such as Spot Counting.
Autoscratch


For use with: Cytation 5 & 7, Lionheart FX, Cytation 1, Lionheart LX

BioTek’s AutoScratch™ Wound Making Tool automatically creates reproducible scratch wounds in cell monolayers grown in microplates. The simple pushbutton operation and tool-free scratch pin manifold exchange make it easy to process either 96 or 24 well plates, which are commonly used in migration and invasion assays. The compact system features an onboard, pre-programmed cleaning routine to keep the scratch pins free of buildup and avoiding contamination. AutoScratch precisely and efficiently automates the sample prep for imaging workflows with BioTek’s Cytation™ Cell Imaging Multi-Mode Readers and Lionheart™ Automated Microscopes. For complete capture through data publication workflows, pre-defined protocols in Gen5™ software automatically calculate wound width, wound % confluence and maximum wound healing rate.
Use AutoScratch with 24-well plates (PN 02638) and 96-well plates (PN 02635).
Gascontroler


Gas Controller for CO2 and O2 controll
For use with Cytation 5 & 7, Lionheart FX, Synergy Neo2, Cytation 1

Increasingly, life science assays are run on live cells. Some of these assays require control over CO2 and O2 concentrations to modulate the environment for pH buffering or to create a hypoxic condition. These assays are traditionally run in flasks in a low-throughput manner, but there is a burgeoning interest in automation of live cell-based assays in microplates for higher throughput and efficiency, therefore there is a need for cell-friendly microplate instrumentation.
The Gas Controller allows full control over CO2 and O2 concentrations to regulate the environment for microplate based live cell assays.
Peltier Cooling Module


The Peltier Cooling Module for BioTek’s Cytation™ 5 and Cytation 1 Cell Imaging Multi-Mode Readers helps maintain temperature stability inside the reading chamber, regardless of changes in ambient (room) temperature and heat produced by electro-mechanical operations within the reader.
The compact Peltier Cooling Module attaches easily to Cytation’s rear case. (See image)

Instrument without Peltier Cooling Module:

Peltier Cooling Module


The instrument’s interior temperature (red) follows ambient room temperature (green). It is also significantly warmer than ambient temperature because of heat produced by the instrument. This can result in assay data variation through the day, or drifts for kinetic assays run at ambient temperature



Instrument with Peltier Cooling Module:

Peltier Cooling Module


The instrument’s interior temperature (blue) is stabilized which results in more consistent assay data, and higher quality room temperature kinetic data
Benefits of the Peltier Cooling Module for Cytation:
• Helps maintain the instrument’s internal ambient temperature stability, with less than 1 °C rise over external ambient, enabling more consistent results
• Quickly reduces internal temperature following incubated assays up to 3x faster, to allow both incubated and ambient assays to be run more efficiently in multi-user environments.


Peltier Cooling Module is available for:
• Cytation Multi-Mode Reader
• Cytation 5 Cell Imaging Multi-Mode Reader
• Cytation 1 Cell Imaging Multi-Mode Reader
  Cytation 7 Cytation 5 Cytation 1
General
     
Detection modes UV-Vis absorbance UV-Vis absorbance UV-Vis absorbance
Fluorescence intensity Fluorescence intensity Fluorescence intensity
Luminescence Luminescence Luminescence
  Fluorescence polarization Fluorescence polarization
  Time-resolved fluorescence Time-resolved fluorescence
  Alpha  
Read methods Endpoint, kinetic, spectral scanning, well area scanning Endpoint, kinetic, spectral scanning, well area scanning Endpoint, kinetic, spectral scanning, well area scanning
Microplate types Monochromator: 6- to 384-well plates Monochromator: 6- to 384-well plates Monochromator: 6- to 384-well plates
     
Imaging: 6- to 1536-well plates Filters: 6- to 1536-well plates Filters: 6- to 1536-well plates
     
  Imaging: 6- to 1536-well plates Imaging: 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer) Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer) Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer)
Take3 Micro-Volume Plates Take3 Micro-Volume Plates Take3 Micro-Volume Plates
Temperature control 4-Zone incubation to 45 °C with Condensation Control; 4-Zone incubation to 65 °C with Condensation Control; 4-Zone incubation to 45 °C with Condensation Control™;
+0.5 °C at 37 °C +0.2 °C at 37 °C Variation +0.2 °C at 37 °C
Cooling Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides internal cooling after incubated processes. Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides internal cooling after incubated processes. Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides internal cooling after incubated processes.
Shaking Linear, orbital, double orbital Linear, orbital, double orbital Linear, orbital, double orbital
Software Gen5™ Microplate Reader and Imager Software included Gen5™ Microplate Reader and Imager Software Gen5™ Microplate Reader and Imager Software
Gen5 Secure for 21 CFR Part 11 compliance (option) Gen5 Image+ and Image Prime software available for full image analysis Gen5 Image+ and Image Prime software available for full image analysis
    Gen5 Secure available for 21 CFR Part 11
  Gen5 Secure for 21 CFR Part 11 compliance (option)  
Automation BioStack and 3rd party automation compatible BioStack and 3rd party automation compatible BioStack and 3rd party automation compatible
     
BioSpa 8 Automated Incubator compatible BioSpa 8 Automated Incubator compatible BioSpa 8 Automated Incubator compatible
CO2 and O2 control (option) Range: 0 - 20% (CO2); 1 - 19% (O2) Range: 0 - 20% (CO2); 1 - 19% (O2) 0 - 20% CO2 control and 1 – 19% O2 control, with optional Gas Controller
     
Models for both CO2 and O2 or CO2 only are available Models for both CO2 and O2 or CO2 only are available Models for both CO2 and O2 or CO2 only are available
Imaging - Inverted Microscope
Imaging mode Fluorescence, color brightfield, user-selectable brightfield/high contrast brightfield Fluorescence, brightfield, high contrast brightfield, color brightfield and phase contrast Fluorescence and high contrast brightfield (2.5x Zeiss, 4x, 10x, 20x, 40x and 60x)
Imaging method Single color, multi-color, time lapse, montage, z-stacking, z-stack montage Single color, multi-color, montage, time lapse, z-stacking Single color, multi-color, montage, time lapse, z-stacking
Image processing Z-projection, digital phase contrast, stitching Z-projection, digital phase contrast, stitching Z-projection, image stitching
Camera 16-bit Sony CMOS wide field of view monochrome camera 16-bit Sony CMOS, standard or wide FOV 16-bit gray scale, Sony
Objective capacity 6-position automated turret for user-replaceable objectives 6-position automated turret for user-replaceable objectives 2 user-replaceable objectives
Objectives available 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x
Phase objectives available   4x, 10x, 20x, 40x  
Image filter cube capacity 4 user-replaceable fluorescence cubes, plus brightfield channel 4 user-replaceable fluorescence cubes plus brightfield channel 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-FRET, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, Tag BFP-FRET, GFP (Ex)-CY5 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex 377/Em 647, Oxidized roGFP2, TRITC DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647 DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647
Imaging LED cubes available 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 554 nm, 590 nm, 623 nm, 655 nm, 740 nm 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions Autofocus, user-trained autofocus, autoexposure, auto-LED intensity Autofocus, auto LED intensity, auto exposure Autofocus, auto LED intensity, auto exposure
Autofocus method Image-based autofocus Image-based autofocus Image-based autofocus
User-trained autofocus User-trained autofocus User-trained autofocus
Laser autofocus (option) Laser autofocus (option) Laser autofocus (option)
Positional controls Software Control Software control Software control
   
Joystick controller (option) Joystick control (option)
Image collection rate Image-based autofocus: Image-based autofocus: Image-based autofocus:
     
96 wells, 1 color (DAPI), 4x, 6 minutes 96 wells, 1 color (DAPI), 4x, 6 minutes 96 wells, 1 color (DAPI), 4x, 6 minutes
  96 wells, 3 colors, 4x, 12 minutes 96 wells, 3 colors, 4x, 12 minutes
Laser autofocus:    
  Laser autofocus:  
96 wells, 1 color (DAPI), 4x, <3 minutes   Laser autofocus:
  96 wells, 1 color (DAPI), 4x, <3 minutes  
    96 wells, 1 color (DAPI), 4x, <3 minutes
  96 wells, 3 colors, 4x, <7 minutes, 30 seconds  
    96 wells, 3 colors, 4x, <7 minutes, 30 seconds
  Burst Mode: 10 fps, single well, single color at <= 5Oms integration time  
    Burst Mode: 10 fps, single well, single color at <= 50ms integration time
Image Analysis Software option Gen5 Image+: Image analysis Gen5 Image+: Image analysis Gen5 Image+: Image analysis
     
Gen5 Image Prime: Advanced image analysis Gen5 Image Prime: Advanced image analysis Gen5 Image Prime: Advanced image analysis
     
Gen5 Secure: 21 CFR Part 11 compliant features Gen5 Secure: 21 CFR Part 11 compliant features Gen5 Secure: 21 CFR Part 11 compliant features
Imaging - Upright Microscope
Imaging modes Reflected color brightfield; transmitted color brightfield    
Imaging methods Single image, montage, time lapse, Z-stacking    
Image processing Z-projection, digital phase contrast, stitching    
Camera 16-bit Sony CMOS wide field of view color camera    
Lenses Finder scope, 2x, 4x, 8x    
Positional controls Software Control    
 
Joystick controller (option)
Image collection rate Entire 100 mm dish @ 1x: <2:40 minutes    
 
Entire microscope slide @1x: <1:15 minutes
 
96-well ELISpot plate@ 1x: <5 minutes
Image Analysis Software option Gen5 Image+: Image analysis    
 
Gen5 Image Prime: Advanced image analysis
 
Gen5 Secure: 21 CFR Part 11 compliant features
Fluorescence Intensity
Light source Xenon flash Xenon flash Xenon flash
Detector PMT (red-shifted PMT option) PMT for monochromator system PMT
 
PMT for filter system
Wavelength selection Quad monochromators (top/bottom) Quad monochromators (top/bottom) Deep blocking band pass filters / dichroic mirrors
 
Filters (top)
Wavelength range 250 – 700 (900 nm option) Monochromators: 250 - 700 nm (900 nm option) Filters: 200 - 700 nm (850 nm option)
 
Filters: 200 - 700 nm (850 nm option)
Monochromator bandwidth Variable, from 9 nm to 50 nm in 1 nm increments Variable, from 9 nm to 50 nm in 1 nm increments  
Dynamic range 7 decades 7 decades 7 decades
Sensitivity Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) – top Filters: Fluorescein: 0.25 pM (0.025 fmol/well, 384-well plate)
   
Fluorescein 4 pM (0.4 fmol/well, 384-well plate) – bottom Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate)
   
   
  Quad Monochromator:
   
  Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) - top
   
  Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom
Reading speed (kinetic) 96 wells: 11 seconds 96 wells: 11 seconds 96 wells: 11 seconds
     
384 wells: 22 seconds 384 wells: 22 seconds 384 wells: 22 seconds
Luminescence      
Wavelength range 300 - 700 nm 300 - 700 nm 300 - 700 nm
Dynamic range >6 decades >6 decades >6 decades
Sensitivity 20 amol ATP (flash) Monos: 20 amol ATP (flash) 10 amol ATP (flash)
   
Filters: 10 amol ATP (flash), 100 amol (glow) 100 amol (glow)
Fluorescence Polarization
Light source   Xenon flash Xenon flash
Detector   PMT PMT
Wavelength selection   Filters Filters
Wavelength range   280 - 700 nm (850 nm option) 400 nm – 700 nm
Sensitivity   1.2 mP standard deviation at 1 nM fluorescein 1.2 mP standard deviation at 1 nM fluorescein
Time-Resolved Fluorescence      
Light source   Xenon flash Xenon flash
Detector   PMT PMT
Wavelength selection   Quad monochromators (secondary mode) Filters
 
Filters (top)
Wavelength range   Filters: 200 - 700 nm (850 nm option)  
Sensitivity   Filters: Europium 40 fM (4 amol/well, 384-well plate) Europium 40 fM (4 amol/well, 384-well plate)
 
Monos: Europium 1200 fM (120 amol/well, 384-well plate)
Absorbance      
Light source Xenon flash Xenon flash Xenon flash
Detector Photodiode photodiode photodiode
Wavelength selection Monochromator Monochromator Monochromator
Wavelength range 230 - 999 nm, 1 nm increment 230 - 999 nm, 1 nm increment 200 – 999 nm, 1 nm increment
Monochromator bandwidth 4 nm (230 - 285 nm), 8 nm (>285 nm) 4 nm (230 - 285 nm), 8 nm (>285 nm) 2.4 nm
Dynamic range 0 - 4.0 OD 0 - 4.0 OD 0 – 4.0 OD
Resolution 0.0001 OD 0.0001 OD 0.0001 OD
Pathlength correction Yes yes yes
Monochromator wavelength accuracy +2 nm +2 nm + 2 nm
Monochromator wavelength repeatability +0.2 nm +0.2 nm + 0.2 nm
OD accuracy <1% at 3.0 OD <1% at 2.0 OD <1% at 2.0 OD
   
<3% at 3.0 OD <3% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD <1% from 0 to 3.0 OD <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD <0.5% at 2.0 OD <0.5% at 2.0 OD
Stray light 0.03% at 230 nm 0.03% at 230 nm 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 11 seconds 96 wells: 11 seconds 96 wells: 11 seconds
     
384 wells: 22 seconds 384 wells: 22 seconds 384 wells: 22 seconds
Alpha Detection
Light source   100 mW 680 nm laser  
Detector   PMT  
Wavelength selection   Filters (top)  
Sensitivity   100 amol LCK peptide (384-well low volume plate)  
Reagent Injectors (option)      
Supported detection modes   All modes All modes
Number 2 syringe pumps 2 syringe pumps 2 syringe pumps
Supported labware 6- to 384-well plates, Petri and cell culture dishes 6- to 384-well plates, Petri and cell culture dishes 6- to 384-well plates, Petri and cell culture dishes
Dead volume 1.1 mL with back flush 1.1 mL with back flush 1.1 mL with back flush
Dispense volume 5 - 1000 µL in 1 µL increments 5 - 1000 µL in 1 µL increments 5 - 1000 µL in 1 µL increments
Plate geometry 6- to 384-well microplates 6- to 384-well microplates 6- to 384-well microplates
Dispense accuracy +1 µL or 2% +1 µL or 2% +1 µL or 2%
Dispense precision <2% at 50 - 200 µL <2% at 50 - 200 µL <2% at 50 – 200 µL
Physical Characteristics
Power 150 Watts maximum consumption 250 Watts maximum consumption. 100-240 VAC, 50/60 Hz (24VDC external power supply, 150W min)
Dimensions 20.2" D x 16.4" W x 17.5" H 20.2" D x 16.4" W x 17.5" H 20.2" D x 16.4" W x 17.5" H
     
(51.4 cm D x 41.6 cm W x 44.5 cm H) (51.4 cm D x 41.6 cm W x 44.5 cm H) (51.4 cm D x 41.6 cm W x 44.5 cm H)
Weight 80 lbs (36.3 kg) 80 lbs (36.3 kg) 65 lbs (29 Kg)
Regulatory      
Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available. CE and TUV marked. RoHS Compliant. Models for In Vitro CE and TUV marked. Models for In Vitro Diagnostic use are available.
Diagnostic use are available.
Specifications are subject to change.