BioTek EL406

Microplate Washer Dispenser

Overview

The EL406 Combination Washer Dispenser offers fast, full plate washing along with three reagent dispensers in one, compact instrument. Its multiple capabilities include washing and dispensing for standard assays, plus biomagnetic separation and vacuum filtration protocols. The patented Dual-Action™ manifold optimizes washing for loosely adherent cell monolayers, while the patented Ultrasonic Advantage™ helps prevent blockages in the manifold tubes before washing, to reduce assay failure. Syringe pump and peristaltic pump dispensers each offer unique advantages, and the EL406 offers both, along with superior plate washing, on a single platform - making the EL406 a workhorse for your laboratory.

• Combines fast microplate washing with 3 reagent dispensers in 1 compact instrument
• 96-, 384- and 1536-well plate compatible
• Gentle processing for cell-based assays
• Bead washing: magnetic and polystyrene
• Easy maintenance with Ultrasonic Advantage™

EL406 Washer Dispenser product video.

BioTek EL406 Microplate Washer Dispenser



Combines fast microplate washing with 3 reagent dispensers in 1 compact instrument
There’s no need for multiple instruments when you have an EL406 in the lab! The EL406 is microplate washer with peristaltic and syringe pump modules available to make it into an efficient multi-tasking system. For benchtop use or integrated into automated systems, the EL406 does the work of multiple instruments.

96-, 384- and 1536-well plate compatible
BioTek’s patented Dual-Action™ manifold quickly washes 96- and 384-well plates. For 1536 washing, unique aspirate and dispense manifolds rapidly dispense and aspirate across the plate. Wash speeds for the three plate types range from 13 seconds to 36 seconds, keeping all your processes efficient.

Gentle processing for cell-based assays
Cell-based assays can be challenging to wash - too often, cell monolayers are disrupted by vigorous dispensing or overly intense aspiration. The EL406 Dual-Action manifold and plate carrier have x-y-z adjustments, and flow rates are fully adjustable - to optimize parameters for even the most sensitive assays. Angled dispense tubes direct fluid flow to the well sides, further minimizing disruption.

Bead washing: magnetic and polystyrene
The EL406 is a powerhouse for many standard plate washing and dispensing protocols, but it also automates more complex operations like magnetic and polystyrene bead washing workflows – and it’s Luminex® xMap® approved. Bead washing is available for 96- and 384-well microplates.

Easy maintenance with Ultrasonic Advantage™
EL406 has a number of automated system maintenance features, including. BioTek’s patented Ultrasonic Advantage™. The dispense and aspirate tubes are immersed into the built-in ultrasonic bath in the priming trough while the automate cleaning program runs, dislodging and aspirating away salt crystal and protein buildup in the tubes.

GENERAL
Microplate types Dispensing (peri pump and syringe)
96-, 384- and 1536-well standard, deep and PCR plates; 6-. 12-, 24- and 48-well plates (dispense tip configurable)
Washing
96-. 384-well standard plates; 6-, 12-, 24-,and 48-well plates (with compatible manifold configuration)
Onboard software Create, edit or run multiple protocols
Software (computer control) LHC2 Software
LHC2 Secure for 21 CFR Part 11 compliance (option)
SiLA Compliant driver (option)
Shaking Programmable up to 60 minutes
Slow, medium, fast or variable
Soaking Programmable up to 60 minutes
USB ports (2) For protocol storage/transfer and for optional external mouse or keyboard
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
WASHING
Manifold types 96- and 384-well washing: 8-tube manifold
6-, 12-, 24-, 48-well washing: Custom manifolds available
Volume range 20-30,000 µL/well
Wash cycles 1-okt
Supply bottle 2 L
Dispense accuracy 3%
Dispense precision 96-/384-well plates, 300 µL/well: <3% CV
6-well plates, 5560 µL/well: <5% CV 
Residual volume 96-well plate, 300 µL/well: < 2 µL/well
Wash speed 96-wells, 8-tube manifold, 3 cycles, >300 µL/well: <130 seconds
Wash volume 20-30,000 µL/well
Waste bottle 2L
Flow rates 140-422 µL/well/second
Sterilization Chemical
DISPENSING - PERISTALTIC PUMP (MULTI-CHANNEL)
Manifold types 1 x 8 - sapphire jeweled 316 SS, 316 SS or polypropylene tips, with 1, 5 or 10 µL tubing
Fluid delivery 1 or 2 peristaltic pumps
Dispense speed 96 wells, 5 µL cass, 10 µL/ well: 3 seconds
96 wells, 5 µL cass, 20 µL/ well: 3.5 seconds
384 wells, 5 µL cass, 5 µL/ well: 6.5 seconds
384 wells, 10 µL cass, 10 µL/ well: 8 seconds
384 wells, 1 µL cass, 1 µL/ well: 6 seconds
1536 wells, 1 µL cass, 1 µL/ well: 21 seconds
Volume range 500 nL - 3,000 µL/well, selectable in 1 µL increments
Flow rates User programmable rates from high to low
Dispense performance 1 µL cassette: recommended range: 1 - 50 µL
Accuracy: +5% at 1 µL
Precision: <5% CV at 1 µL
<10% CV at 500 nL
Minimum Prime Volume: 1.20 mL
 
5 µL cassette: recommended range: 5 - 2,500 µL
Accuracy: +2.0% at 5 µL
Precision: <2.5% CV at 5 µL
Minimum Prime Volume: 4.23 mL
 
10 µL cassette: recommended range: 10 - 3,000 µL
Dispense Accuracy: +2.0% at 10 µL
Dispense Precision: <2.0% CV at 10 µL
Minimum Prime Volume: 7.36 mL
Recommended cassette replacement interval 1 µL Cassette: 1,000 384-well microplates at 5 µL well
5 µL Cassette: 1,000 96-well microplates at 50 µL well
10 µL Cassette: 1,000 96-well microplates at 100 µL well
Sterilization Autoclave, chemical
DISPENSING - SYRINGE PUMP
Manifold types 96- and 384-well dispensing:
One 16-tube (2 x 8) manifolds - 316 SS tubes
Two 16-tube (1 x 16) manifolds - 316 SS tubes
 
1536-well dispensing:
Two 32-tube (1 x 32) manifolds - sapphire jeweled 316 SS or 316 SS tubes
6- to 48-well dispensing: custom autoclavable manifolds available
Fluid delivery Two positive displacement syringe drives
Dispense speed 20 µL /well, 96 wells, 1 x16 tubes:: 5 seconds
20 µL /well, 384 wells, 1 x16 tubes:: 14 seconds
3 µL /well, 1536 wells, 2 x 32 tubes:: 7 seconds
Volume range 3 - 3,000 µL/well selectable in 1 µL increments
Minimum Prime Volume: 12 mL
Flow rates User programmable rates from high to low
Dispense accuracy +1 µL at 5 µL
+1 µL at 20 µL
+1% at 100 µL
Dispense precision <5% CV at 5 µL
<2.5% CV at 20 µL
<1% CV at 100 µL
Supply bottle 1 L or 2 L
Sterilization Chemical, autoclavable option
DISPENSING - PERISTALTIC PUMP (RAD TECHNOLOGY)
Labware types Single tip: 6-, 12-, 24-, 48-, 96-, 384-well plates; low profile
standard height and deep well formats; PCR trays and microtubes
8-to-1 tip: 6-, 12-, and 24-well plates
Manifold Types RAD single, with plastic or steel tip with 1, 5 or 10 tubing, 7° angle
RAD 8-to-1 plastic tip, with 5 µL tubing, angled bulk dispense chute
Volume range 500 nL - 30,000 µL
Minimum prime volume 1 µL cass, 18": 90 µL ; 1 µL cass, 30": 150 µL
5 µL cass, 18": 320 µL; 1 µL cass, 30": 530 µL
10 µL cass, 18": 555 µL; 10 µL cass, 30": 920 µL
Dispense speed (high flow) 1 µL cass, 1 µL/well: 19s (96 wells) 55s (384 wells)
1 µL cass, 10 µL/well: 33s (96 wells), 112s (384 wells)
5 µL cass, 5 µL/well: 19s (96 wells), 58s (384 wells)
5 µL cass, 100 µL/well: 76s (96 wells), 286s (384 wells)
10 µL cass, 10 µL/well: 21s (96 wells), 66s (384 wells)
10 µL cass, 100 µL/well: 70s (96 wells), 259s (384 wells)
Dispense performance 1 µL cass (med), 0.5 µL/well: Precision 10% CV
1 µL cass (med), 1 µL/well: Accuracy + 10%, Precision 10% CV
1 µL cass (med), >2 µL/well: Accuracy + 5%, Precision 5% CV
5 µL cass (high), 5 µL/well: Accuracy + 4%, Precision 5% CV
5 µL cass (high), >10 µL/well: Accuracy + 2%, Precision 2.5% CV
10 µL cass (high), 10 µL/well: Accuracy + 4%,Precision 4% CV
10 µL cass (high), >20 µL/well: Accuracy + 2%, Precision 2 %CV
8-to 1 cass(high), 40 µL/well: Accuracy + 4%
8-to 1 cass (high), >10 µL/well: Precision 2.5%CV
8- to 1cass(high), >80 µL/well: Accuracy + 2%
PHYSICAL CHARACTERISTICS
Power 100 - 240 Volts AC. 50/60 Hz
Dimensions Base instrument: 17.19"W x 11.75" D x 8" H (43.51 x 29.21 x 20.32 cm)
Weight Base instrument:
19.5 lbs (8.8 Kg)
REGULATORY
Regulatory CE and TUV marked, RoHS Compliant. Models for In Vitro Diagnostic use are available.
Part #
1536-/ 384-/ 96-well
1536-/ 384-well
1536-well
384-/ 96-well
384-well
96-well
3 reagent disp.
2 reagent disp.
1 reagent disp.
406PSUB3SB
         
   
406PSUB3LB
         
   
406PSUB1SB
         
   
406PSUB1LB
         
   
406PSUB2SB  
       
   
406PSUB2LB  
       
   
406PSU0SB    
     
   
406PSU0LB    
     
   
406SNA0LB    
       
 
406PSUB3      
   
   
406PSUB1      
   
   
406PUB3      
       
406PUB1      
       
406PSUB2        
 
   
406PUB2        
     
406PSNA4          
   
406SNA4          
 
 
406P4          
   


EL406™ 1536-/384-/96-well Microplate Washer Dispenser Configurations
  Feature Design
P 1-Reagent Dispense Module – Autoclavable
1 Peristaltic Pump 8-tip (1 x 8) cassette
  Sapphire jeweled, steel or plastic tips
S 2-Reagent Dispense Module – Autoclavable
2 Syringe Pumps 2 16-tube (1 x 16) manifolds
  Steel tubes
SNA 2-Reagent Dispense Module – Non-autoclavable
2 Syringe Pumps 1 16-tube (2 x 8) manifolds - except with P/N 406SNA0-LB
  Steel tubes
U Wash Module – Ultrasonic Advantage™  
B Wash Module – Automatic 4-Buffer Switching  
1 Wash Module – 384-/96-well microplate washing Dual-Action™
96-tube (8 x 12) manifold
Steel tubes
2 Wash Module – faster 384-well microplate washing Dual-Action™
192-tube (16 x 12) manifold
Steel tubes
3 Wash Module – 384-/96-well microplate washing Dual-Action™
Interchangeable 96-tube (384-/96- well washing) and 192-tube (faster 384-well washing) manifolds
Steel tubes
4 Wash Module – 96-well microplate washing 96-tube (8 x 12) manifold
Steel tubes
SB Wash Module – 1536-well microplate washing and dispensing 2 32-tube (1 x 32) dispense manifolds
Small bore, sapphire jeweled tubes
128-tube (4 x 32) aspiration manifold
LB Wash Module – 1536-well microplate washing and dispensing 2 32-tube (1 x 32) dispense manifolds
Large bore, steel tubes
128-tube (4 x 32) aspiration manifold
LHC


The EL406 has extensive onboard software, with an intuitive menu-driven interface. Program all aspects of the system’s dispense, wash and maintenance parameters, quickly and easily. Up to 75 programs can be stored onboard.

LHC
LHC Software allows computer-based programming of multi-step processes and it is used for integrating BioTek’s multi-mode washer dispensers, washers and dispensers into robotic systems. You can operate the system under computer control via LHC, or create protocols in LHC and download to the instrument for recall and execution.

LHC Secure software
LHC Secure software offers features to facilitate compliance to FDA directive 21 CFR Part 11
with computer control of BioTek’s Multi-Mode Washer Dispensers, Washers and Dispensers.

LHC SiLA software
LHC SiLA softwaredriver enables BioTek washer and dispenser integration into SiLA compliant automated systems. Includes LHC software for computer control and third party integration of BioTek’s Multi-Mode Washer Dispensers, Washers and Dispensers.