imaging

BioTek Automated Imaging & (confocal) Microscopy Systems

Endpoint and/or Live Cell Imaging

Overview

BioTek’s automated imagers bring your science to life, capturing spectacular images, z-stacks, montages and time lapse sequence with ease. With up to 100x magnification, plus brightfield, color brightfield, phase contrast and fluorescence channels, Lionheart Automated Microscopes support a wide range of microscopy workflows.


Cytation C10 Confocal Imaging Reader

Cytation™ C10 Confocal Imaging Reader combines automated digital confocal and widefield microscopy with conventional multi-mode microplate reading in a unique, patented design. The spinning disk confocal module provides exquisite resolution and optical sectioning capabilities in a wide variety of sample types. High quality components used in the system, including a Hamamatsu scientific CMOS (sCMOS) camera, Olympus objectives and laser-based illumination enable excellent image quality, at a significantly lower price than others. Cytation C10 also includes widefield fluorescence, brightfield and phase contrast optics. Its variable bandwidth monochromator-based multi-mode plate reading is based on the proven design of the market-leading BioTek Synergy™ products. Environmental controls enable live cell assays, and Gen5™ software is specifically designed to make sample detection and image capture quick and effortless. The new 3D viewer provides users with 3D reconstruction of their thicker samples captured with the confocal microscope. Cytation C10 brings affordable confocal to every laboratory.

• BioTek Cytation C10 Confocal Imaging Reader

Cytation: Cell Imaging - Multi-Mode Readers:

The Cytation range combines automated digital microscopy and conventional microplate reader detection in a modular, upgradable platform. This patented design provides rich phenotypic cellular information with well-based intensity data.

• BioTek Cytation 7 Cell Imaging Multi-Mode Reader
• BioTek Cytation 5 Cell Imaging Multi-Mode Reader
• BioTek Cytation 1 Cell Imaging Multi-Mode Reader



Lionheart: Automated Imagers:


The standalone Lionheart imagers are digital microscopes that automates image capture and analysis for a wide range of microscopy workflows.

• Lionheart™ FX Automated Live Cell Microscope
• Lionheart™ LX Automated Endpoint Microscope



Please contact us to discuss which type is the best fit for your laboratory.



Lionheart & Cytation Comparison Table



Download the comparison as pdf document















  Lionheart FX Lionheart LX Cytation C10 Cytation 7 Cytation 5 Cytation 1 BioSpa System
General              
Detection modes     UV-Vis absorbance UV-Vis absorbance UV-Vis absorbance UV-Vis absorbance  
       
Fluorescence intensity Fluorescence intensity Fluorescence intensity Fluorescence intensity
       
Luminescence Luminescence Luminescence Luminescence
       
    Fluorescence polarization Fluorescence polarization
       
    Time-resolved fluorescence Time-resolved fluorescence
       
    Alpha  
Read methods     Endpoint, kinetic, spectral scanning, well area scanning Endpoint, kinetic, spectral scanning, well area scanning Endpoint, kinetic, spectral scanning, well area scanning Endpoint, kinetic, spectral scanning, well area scanning  
Microplate types 6- to 1536-well plates 6- to 1536-well plates Monochromator: 6- to 384-well plates Monochromator: 6- to 384-well plates Monochromator: 6- to 384-well plates Monochromator: 6- to 384-well plates 6- to 1536-well standard height microplates, with or without lids.
         
Imaging: 6- to 1536-well plates Imaging: 6- to 1536-well plates Filters: 6- to 1536-well plates Filters: 6- to 1536-well plates Plate height range: 7.6 mm to 25.4 mm
         
    Imaging: 6- to 1536-well plates Imaging: 6- to 1536-well plates  
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer), Take3 Micro-volume plates Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer) Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer) Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer) Cell culture dishes (60 mm and 35 mm)
         
Support for labware up to 1.5" tall Support for labware up to 1.5" tall Take3 Micro-Volume Plates Take3 Micro-Volume Plates Take3 Micro-Volume Plates
Lidded plate handling             BioSpa automated plate movement, including de-lidding and re-lidding between the incubator and imager.
Microplate capacity             Up to 8 microplates (or other labware)
Air filter             User-replaceable HEPA filter
Compatible instruments             Cytation 7, Cytation 5, Cytation 1, EL406, 405 TS, MultiFlo FX
Temperature control Incubation to 40 °C with optional environmental control cover   4-Zone incubation to 45 °C with Condensation Control 4-Zone incubation to 45 °C with Condensation Control 4-Zone incubation to 65 °C with Condensation Control; 4-Zone incubation to 45 °C with Condensation Control™; Range: to 45 °C
+0.2 °C at 37 °C Variation +0.2 °C at 37 °C  
    Control resolution: 0.1 °C
     
    Uniformity + 0.5 °C at 37 °C
Cooling       Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides internal cooling after incubated processes. Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides internal cooling after incubated processes. Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides internal cooling after incubated processes.  
Shaking     Linear, orbital, double-orbital Linear, orbital, double orbital Linear, orbital, double orbital Linear, orbital, double orbital  
Software Gen5 Microplate Reader and Imager Software included Gen5 Microplate Reader and Imager Software included Gen5 Microplate Reader and Imager Software included Gen5™ Microplate Reader and Imager Software included Gen5™ Microplate Reader and Imager Software included Gen5™ Microplate Reader and Imager Software included Allows user notification (text or email) of events and/or errors in the system
             
Gen5 Image+ and Image Prime Software available for advanced image analysis (option) Gen5 Image+ and Image Prime Software available for advanced image analysis (option) Gen5 Secure for 21 CFR Part 11 compliance (option) Gen5 Secure for 21 CFR Part 11 compliance (option)<br. Gen5 Image+ and Image Prime software available for full image analysis Gen5 Image+ and Image Prime software available for full image analysis Provides control, monitoring and logging of CO2/O2 and incubator temperature
       Gen5 Image+ and Image Prime software available for full image analysis (option)</br.      
Gen5 Secure for 21 CFR Part 11 Compliance (option) Gen5 Secure for 21 CFR Part 11 Compliance (option) Gen5 Image+ and Image Prime software available for full image analysis (option)   Gen5 Secure for 21 CFR Part 11 compliance (option) Gen5 Secure available for 21 CFR Part 11 Provides humidity level monitoring and logging
             
            BioSpa OnDemand mode for simplified, intuitive workflows for single or multiple users
             
            BioSpa Session mode for all imaging, detection and liquid handling operations
Automation     BioStack and 3rd party automation compatible BioStack and 3rd party automation compatible BioStack and 3rd party automation compatible BioStack and 3rd party automation compatible  
     
BioSpa 8 Automated Incubator compatible BioSpa 8 Automated Incubator compatible BioSpa 8 Automated Incubator compatible
CO2 and O2 control (option) 0 - 20% CO2 control and 1 - 19% O2 control, with optional Gas Controller   Range: 0 - 20% (CO2); 1 - 19% (O2), with optional Gas Controller Range: 0 - 20% (CO2); 1 - 19% (O2), with optional Gas Controller Range: 0 - 20% (CO2); 1 - 19% (O2), with optional Gas Controller 0 - 20% CO2 control and 1 – 19% O2 control, with optional Gas Controller Range: 0 - 20% (CO2); 1 - 19% (O2)
         
Models for both CO2 and O2 or CO2 only are available Models for both CO2 and O2 or CO2 only are available Models for both CO2 and O2 or CO2 only are available Models for both CO2 and O2 or CO2 only are available Control Resolution: +0.1% (CO2 and O2)
         
        Stability: +0.2% at 5% CO2; +0.2% at 1% O2
Environmental control cover Top cover for light tight imaging and incubation control (option)            
X/Y Stage resolution Lead screw driven stage with 0.1 µm resolution Lead screw driven stage with 0.1 micron resolution          
Humidity control Humidity chamber with rapid gas recharge (option)           rH: 80 to 95% (lidded plates and 5% CO2)
 
Source: Removable water pan
 
Water level sensor: Low water level alert
Imaging - Confocal Microscope              
Imaging modes     Fluorescence        
Image processing     Z-projection, digital phase contrast, stitching        
Camera     Hamamatsu Orca sCMOS, 16-bit grayscale camera or        
 
Sony CMOS 16-bit grayscale camera
Objective capacity     6-position automated turret for user-replaceable objectives        
Objectives available     20x, 40x, 60x        
Image filter cube capacity     4 user-replaceable fluorescence cubes        
Imaging filter cubes available     CFP, CY5, DAPI, GFP, RFP, TRITC, brightfield        
Laser     6-line        
Automated functions     Autofocus, user-trained autofocus, autoexposure, auto-LED intensity        
Autofocus method     Image-based autofocus        
 
User-trained autofocus
 
Laser autofocus (option)
Positional controls     Software Control        
 
Joystick controller (option)
Imaging methods     Single color, multi-color, time lapse, montage, z-stacking, z-stack montage        
Field of view     Hamamatsu sCMOS: 0.65 mm + 5% at 20x magnification        
 
Sony CMOS: 0.70 mm + 5% at 20x magnification
Image collection rate     Laser autofocus, 0 ms delay, 96 wells: 8 mins, 9 secs        
 
Software autofocus, 0 ms delay, 96 wells: 12 mins, 1 sec
Imaging - Widefield Microscope              
Imaging mode Fluorescence, brightfield, high contrast brightfield, color brightfield and phase contrast Fluorescence, high contrast brightfield and color brightfield Fluorescence, phase contrast, color brightfield, user-selectable brightfield/high contrast brightfield Fluorescence, color brightfield, user-selectable brightfield/high contrast brightfield Fluorescence, phase contrast, brightfield, high contrast brightfield, color brightfield and phase contrast Fluorescence and high contrast brightfield (2.5x Zeiss, 4x, 10x, 20x, 40x and 60x) Fluorescence, brightfield, high contrast brightfield, color brightfield and phase contrast
Imaging method Single color, multi-color, montage, time lapse, Z-stacking, burst mode Single color, multi-color, montage, time lapse, Z-stack, z-stack montage, burst mode Single color, multi-color, time lapse, montage, z-stacking, z-stack montage Single color, multi-color, time lapse, montage, z-stacking, z-stack montage Single color, multi-color, montage, time lapse, z-stacking Single color, multi-color, montage, time lapse, z-stacking Single color, multi-color, montage, time lapse, z-stacking
Light source High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm) High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm,          
590 nm, 623 nm, 655 nm, 740 nm)
Image processing     Z-projection, digital phase contrast, stitching Z-projection, digital phase contrast, stitching Z-projection, digital phase contrast, stitching Z-projection, image stitching Z-projection, digital phase contrast, stitching
Camera 16-bit grayscale Sony CMOS 16-bit grayscale Sony CMOS Hamamatsu Orca sCMOS, 16-bit grayscale camera or Sony CMOS, 16-bit grayscale Sony CMOS, 16-bit grayscale, standard or WFOV Sony CCD, 16-bit gray scale 16-bit gray scale Sony
 
Sony CMOS 16-bit grayscale camera
Camera binning Optional 2x2 binning for focus and/or image capture Optional 2x2 binning for focus and/or image capture          
Camera exposure range 5 milliseconds to 4 seconds 5 milliseconds to 4 seconds          
Image outputs available Raw Images: 16-bit TIFF Raw Images: 16-bit TIFF          
   
Saved Images: TIF, JPG, BMP, PNG, EMF, GIF Saved Images: TIFF, JPG, BMP, PNG, EMF, GIF, color TIFF
   
Movies: MP4, WMV Movies: MP4, WMV
Objective capacity 6 onboard, user-replaceable objectives 6 onboard, user-replaceable objectives 6-position automated turret for user-replaceable objectives 6-position automated turret for user-replaceable objectives 6-position automated turret for user-replaceable objectives 2-position automated turret for user-replaceable objectives 6 user-replaceable objectives
Objectives available Fluorescence: Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA.60; 60x, NA .70 1.25x, 2.5x (2.25x eff), 4x,10x, 20x, 40x, 60x 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x 1.25x, 2.5x, 4x, 10x, 20x, 40x, 60x
   
Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30; Oil: 60x, NA:1.42; 60x, NA 1.25; 100x, NA 1.4; 100x, NA 1.3
   
20x, NA: .45; 40x, NA.60; 60x, NA .70 High NA: 20x, NA 0.75; 40x, NA 0.95
   
Oil: 60x, NA:1.42; 100x, NA: 1.40  
   
Phase objectives available: 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA .60  
Phase objectives available     4x, 10x, 20x, 40x   4x, 10x, 20x, 40x   4x, 10x, 20x, 40x
Image filter cube capacity 4 user-replaceable fluorescence cubes plus brightfield channel 4 user-replaceable fluorescence cubes plus brightfield channel 4 user-replaceable fluorescence cubes, plus brightfield channel 4 user-replaceable fluorescence cubes, plus brightfield channel 4 user-replaceable fluorescence cubes plus brightfield channel 4 user-replaceable fluorescence cubes plus brightfield channel 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647 DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647 DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-FRET, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, Tag BFP-FRET, GFP (Ex)-CY5 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex 377/Em 647, Oxidized roGFP2, TRITC DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-FRET, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, Tag BFP-FRET, GFP (Ex)-CY5 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex 377/Em 647, Oxidized roGFP2, TRITC DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647 DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647 More than 20 colors are available
Imaging LED cubes available     365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 554 nm, 590 nm, 623 nm, 655 nm, 740 nm 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 554 nm, 590 nm, 623 nm, 655 nm, 740 nm 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions Autofocus, user-trained autofocus, auto exposure, auto-LED intensity Autofocus, user-trained autofocus, auto exposure, auto-LED intensity Autofocus, autoexposure, auto-LED intensity Autofocus, autoexposure, auto-LED intensity Autofocus, auto LED intensity, auto exposure Autofocus, auto LED intensity, auto exposure User-trained autofocus, autofocus, autoexposure, auto-LED intensity
Autofocus method Image-based autofocus Image-based autofocus Image-based autofocus Image-based autofocus Image-based autofocus Image-based autofocus Image-based autofocus
             
Laser autofocus option Laser autofocus option User-trained autofocus User-trained autofocus User-trained autofocus User-trained autofocus User-trained autofocus
             
    Laser autofocus (option) Laser autofocus (option) Laser autofocus (option) Laser autofocus (option) Laser autofocus (option)
Positional controls     Software Control Software Control Software control Software control  
     
Joystick controller (option) Joystick controller (option) Joystick control (option)
Image collection rate Single well fastest frame rate capture: Single well fastest frame rate capture: Image-based autofocus: Image-based autofocus: Image-based autofocus: Image-based autofocus:  
           
*Full Resolution: up to 10 frames per second for single color images *Full Resolution: up to 10 frames per second for single color images 96 wells, 1 color (DAPI), 4x, 6 minutes 96 wells, 1 color (DAPI), 4x, 6 minutes 96 wells, 1 color (DAPI), 4x, 6 minutes 96 wells, 1 color (DAPI), 4x, 6 minutes
           
*2x2 Binning: up to 20 frames per second for single color images *2x2 Binning: up to 20 frames per second for single color images Laser autofocus:   96 wells, 3 colors, 4x, 12 minutes 96 wells, 3 colors, 4x, 12 minutes
           
    96 wells, 1 color (DAPI), 4x, <3 minutes Laser autofocus:    
        Laser autofocus:  
      96 wells, 1 color (DAPI), 4x, <3 minutes   Laser autofocus:
        96 wells, 1 color (DAPI), 4x, <3 minutes  
          96 wells, 1 color (DAPI), 4x, <3 minutes
        96 wells, 3 colors, 4x, <7 minutes, 30 seconds  
          96 wells, 3 colors, 4x, <7 minutes, 30 seconds
        Burst Mode: 10 fps, single well, single color at <= 5Oms integration time  
          Burst Mode: 10 fps, single well, single color at <= 50ms integration time
Image Analysis Software option     Gen5 Image+: Image analysis Gen5 Image+: Image analysis Gen5 Image+: Image analysis Gen5 Image+: Image analysis  
       
Gen5 Image Prime: Advanced image analysis Gen5 Image Prime: Advanced image analysis Gen5 Image Prime: Advanced image analysis Gen5 Image Prime: Advanced image analysis
       
Gen5 Secure: 21 CFR Part 11 compliant features Gen5 Secure: 21 CFR Part 11 compliant features Gen5 Secure: 21 CFR Part 11 compliant features Gen5 Secure: 21 CFR Part 11 compliant features
Microscope stage control Gen5 Software Control Gen5 Software Control          
   
Optional joystick controller Optional joystick controller
Liquid handling             See the individual product pages at EL406, MultiFlo FX and 405 TS
Imaging - Upright Microscope              
Imaging modes       Reflected color brightfield; transmitted color brightfield      
Imaging methods       Single image, montage, time lapse, Z-stacking      
Image processing       Z-projection, digital phase contrast, stitching      
Camera       16-bit Sony CMOS color camera      
Lenses       Finder scope, 2x, 4x, 8x      
Positional controls       Software Control      
 
Joystick controller (option)
Image collection rate       Entire 100 mm dish (montage) @ 1x: <3:00 minutes      
 
Entire microscope slide (montage) @ 1x: <1:15 minutes
 
96-well ELISpot plate @ 1x: <5 minutes
Image Analysis Software option       Gen5 Image+: Image analysis      
 
Gen5 Image Prime: Advanced image analysis
 
Gen5 Secure: 21 CFR Part 11 compliant features
Fluorescence Intensity              
Light source     Xenon flash Xenon flash Xenon flash Xenon flash  
Detector     PMT PMT PMT for monochromator system PMT  
 
PMT for filter system
Wavelength selection     Quad monochromators (top/bottom) Quad monochromators (top/bottom) Quad monochromators (top/bottom) Deep blocking band pass filters / dichroic mirrors  
 
Filters (top)
Wavelength range     250 – 700 nm (900 nm option) 250 – 700 (900 nm option) Monochromators: 250 - 700 nm (900 nm option) Filters: 200 - 700 nm (850 nm option)  
 
Filters: 200 - 700 nm (850 nm option)
Monochromator bandwidth     Variable, from 9 nm to 50 nm in 1 nm increments Variable, from 9 nm to 50 nm in 1 nm increments Variable, from 9 nm to 50 nm in 1 nm increments    
Dynamic range     7 decades 7 decades 7 decades 7 decades  
Sensitivity       Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) – top Filters: Fluorescein: 0.25 pM (0.025 fmol/well, 384-well plate)  
   
Fluorescein 4 pM (0.4 fmol/well, 384-well plate) – bottom Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate)
   
   
  Quad Monochromator:
   
  Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) - top
   
  Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom
Reading speed (kinetic)     96 wells, sweep mode: 10 seconds 96 wells: 11 seconds 96 wells: 11 seconds 96 wells: 11 seconds  
       
384 wells, sweep mode: 20 second 384 wells: 22 seconds 384 wells: 22 seconds 384 wells: 22 seconds
Luminescence              
Wavelength range     300 - 700 nm 300 - 700 nm 300 - 700 nm 300 - 700 nm  
Dynamic range     >6 decades >6 decades >6 decades >6 decades  
Sensitivity       20 amol ATP (flash) Monos: 20 amol ATP (flash) 10 amol ATP (flash)  
   
Filters: 10 amol ATP (flash), 100 amol (glow) 100 amol (glow)
Fluorescence Polarization              
Light source         Xenon flash Xenon flash  
Detector         PMT PMT  
Wavelength selection         Filters Filters  
Wavelength range         280 - 700 nm (850 nm option) 280 – 700 nm (850 nm option)  
Sensitivity         1.2 mP standard deviation at 1 nM fluorescein 1.2 mP standard deviation at 1 nM fluorescein  
Time-Resolved Fluorescence              
Light source         Xenon flash Xenon flash  
Detector         PMT PMT  
Wavelength selection         Quad monochromators (secondary mode) Filters  
 
Filters (top)
Wavelength range         Filters: 200 - 700 nm (850 nm option)    
Sensitivity         Filters: Europium 40 fM (4 amol/well, 384-well plate) Europium 40 fM (4 amol/well, 384-well plate)  
 
Monos: Europium 1200 fM (120 amol/well, 384-well plate)
Absorbance              
Light source     Xenon flash Xenon flash Xenon flash Xenon flash  
Detector     Photodiode Photodiode Photodiode Photodiode  
Wavelength selection     Monochromator Monochromator Monochromator Monochromator  
Wavelength range     230 - 999 nm, 1 nm increments 230 - 999 nm, 1 nm increment 230 - 999 nm, 1 nm increment 200 – 999 nm, 1 nm increment  
Monochromator bandwidth     4 nm (230 - 285 nm), 8 nm (>285 nm) 4 nm (230 - 285 nm), 8 nm (>285 nm) 4 nm (230 - 285 nm), 8 nm (>285 nm) 2.4 nm  
Dynamic range     0 - 4.0 OD 0 - 4.0 OD 0 - 4.0 OD 0 – 4.0 OD  
Resolution     0.0001 OD 0.0001 OD 0.0001 OD 0.0001 OD  
Pathlength correction     Yes Yes yes yes  
Monochromator wavelength accuracy     + 2 nm +2 nm +2 nm + 2 nm  
Monochromator wavelength repeatability     + 0.2 nm +0.2 nm +0.2 nm + 0.2 nm  
OD accuracy     <1% at 3.0 OD <1% at 3.0 OD <1% at 2.0 OD <1% at 2.0 OD  
   
<3% at 3.0 OD <3% at 3.0 OD
OD linearity     <1% from 0 to 3.0 OD <1% from 0 to 3.0 OD <1% from 0 to 3.0 OD <1% from 0 to 3.0 OD  
OD repeatability     <0.5% at 2.0 OD <0.5% at 2.0 OD <0.5% at 2.0 OD <0.5% at 2.0 OD  
Stray light     0.03% at 230 nm 0.03% at 230 nm 0.03% at 230 nm 0.03% at 230 nm  
Reading speed (kinetic)     96 wells: 10 seconds 96 wells: 11 seconds 96 wells: 11 seconds 96 wells: 11 seconds  
       
384 wells: 20 seconds 384 wells: 22 seconds 384 wells: 22 seconds 384 wells: 22 seconds
Alpha Detection              
Light source         100 mW 680 nm laser    
Detector         PMT    
Wavelength selection         Filters (top)    
Sensitivity         100 amol LCK peptide (384-well low volume plate)    
Reagent Injectors (option)              
Supported detection modes     All modes   All modes All modes  
Number 2 syringe pumps   2 syringe pumps 2 syringe pumps 2 syringe pumps 2 syringe pumps  
Supported labware 6- to 384-well microplates, Petri and cell culture dishes, chamber slides   6- to 384-well plates, Petri and cell culture dishes 6- to 384-well plates, Petri and cell culture dishes 6- to 384-well plates, Petri and cell culture dishes 6- to 384-well plates, Petri and cell culture dishes  
Dead volume <1.65 mL with back flush   1.1 mL, with back flush 1.1 mL with back flush 1.1 mL with back flush 1.1 mL with back flush  
Dispense tip options Aligned tip - aligned with optical path for dispensing for fast kinetic assays            
 
Offset tip - dispensing is offset from the optical path
Dispense volume 5 - 1000 µL in 1 µL increments   5 – 1000 µL in 1 µL increments 5 - 1000 µL in 1 µL increments 5 - 1000 µL in 1 µL increments 5 - 1000 µL in 1 µL increments  
Plate geometry     6- to 384-well microplates 6- to 384-well microplates 6- to 384-well microplates 6- to 384-well microplates  
Dispense accuracy +1 µL or 2%   +1 µL or 2% +1 µL or 2% +1 µL or 2% +1 µL or 2%  
Dispense precision <2% at 50 - 200 µL   < 2% at 50 - 200 µL <2% at 50 - 200 µL <2% at 50 - 200 µL <2% at 50 – 200 µL  
Physical Characteristics              
Power 250 Watts maximum consumption. 60 Watts maximum consumption. Instrument: External 250W (minimum), 24VDC power supply, compatible with 100-240 VAC @50-60Hz. 150 Watts maximum consumption 250 Watts maximum consumption. 100-240 VAC, 50/60 Hz (24VDC external power supply, 150W min)  
 
Optional 6-channel laser light source: External 250W power supply, compatible with 100- 240VAC @ 50-60Hz.
 
Optional Hamamatsu scientific camera: External 75W power supply, compatible with 100- 240VAC @ 50-60Hz.
Dimensions With cover closed or without cover: 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm) 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm) 27" W x 18.5" H x 20" D 16.4" W x 17.5" H x 20.2" D 16.4" W x 17.5" H x 20.2" D 16.4" W x 17.5" H x 20.2" D  
    (41.6 cm x 44.5 cm x 51.4 cm)    
With cover fully open: 18.3" D, 17.9" W, 27.5" H (46.5 cm x 45.5 cm x 69.8 cm) (68.6 cm x 45.72 cm x 50.8 cm)   (41.6 cm x 44.5 cm x 51.4 cm) (41.6 cm x 44.5 cm x 51.4 cm)
Weight Without environmental control cover: 51 lbs (23.1 kg) 51 lbs (23.1 kg) 122 lbs (53.3 Kg) 80 lbs (36.3 kg) 80 lbs (36.3 kg) 65 lbs (29 Kg)  
 
With environmental control cover: 58 lbs (26.3 kg)
Regulatory              
Regulatory CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available. CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available. CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available. CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available. CE and TUV marked. RoHS Compliant. Models for In Vitro CE and TUV marked. Models for In Vitro Diagnostic use are available.
Diagnostic use are available.