BioTek Automated Imaging & Microscopy Systems

Endpoint and/or Live Cell Imaging

Overview

BioTek’s automated Microscopes offers a wide range of applications and budgets, from low throughput to high throughput screening (HTS). Assays can vary from simple fixed cells assays to long term live cell kinetic assays or 3D spheroid workflows. All imagers are compact, modular and have the ability to automatically perform augmented microscopy™.


imaging


Imaging Applications

Researchers use BioTek’s imagers for a diverse set of applications, including:



imaging


Our Live cell imaging systems are suitable for assays with a wide range of time resolution, including:



• Calcium imaging (seconds)
• Nuclear localization (minutes)
• Cell migration (hours)
• Cell proliferation (days)
• Tumor invasion (weeks)

imaging



Microscope Models

We offer five microscopes that are all available in multiple configurations to meet the specific criteria of your lab.

Cytation: Cell Imaging - Multi-Mode Readers:
The Cytation range combines automated digital microscopy and conventional microplate reader detection in a modular, upgradable platform. This patented design provides rich phenotypic cellular information with well-based intensity data.

• BioTek Cytation 7 Cell Imaging Multi-Mode Reader
• BioTek Cytation 5 Cell Imaging Multi-Mode Reader
• BioTek Cytation 1 Cell Imaging Multi-Mode Reader

Lionheart: Automated Imagers:
The standalone Lionheart imagers are digital microscopes that automates image capture and analysis for a wide range of microscopy workflows.

• Lionheart™ FX Automated Live Cell Microscope
• Lionheart™ LX Automated Endpoint Microscope



Please contact us to discuss which type is the best fit for your laboratory.



Lionheart & Cytation Comparison Table


Lionheart FX Lionheart LX Cytation 7 Cytation 5 Cytation 1
General          
Detection modes     UV-Vis absorbance UV-Vis absorbance UV-Vis absorbance
     
Fluorescence intensity Fluorescence intensity Fluorescence intensity
     
Luminescence Luminescence Luminescence
     
  Fluorescence polarization Fluorescence polarization
     
  Time-resolved fluorescence Time-resolved fluorescence
     
  Alpha  
Read methods     Endpoint, kinetic, spectral scanning, well area scanning Endpoint, kinetic, spectral scanning, well area scanning Endpoint, kinetic, spectral scanning, well area scanning
Microplate types 6- to 1536-well plates 6- to 1536-well plates Monochromator: 6- to 384-well plates Monochromator: 6- to 384-well plates Monochromator: 6- to 384-well plates
     
Imaging: 6- to 1536-well plates Filters: 6- to 1536-well plates Filters: 6- to 1536-well plates
     
  Imaging: 6- to 1536-well plates Imaging: 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer) Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer) Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer)
         
Support for labware up to 1.5" tall Support for labware up to 1.5" tall Take3 Micro-Volume Plates Take3 Micro-Volume Plates Take3 Micro-Volume Plates
Temperature control Incubation to 40 °C with optional environmental control cover   4-Zone incubation to 45 °C with Condensation Control; 4-Zone incubation to 65 °C with Condensation Control; 4-Zone incubation to 45 °C with Condensation Control™;
+0.5 °C at 37 °C +0.2 °C at 37 °C Variation +0.2 °C at 37 °C
Cooling     Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides internal cooling after incubated processes. Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides internal cooling after incubated processes. Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides internal cooling after incubated processes.
Shaking     Linear, orbital, double orbital Linear, orbital, double orbital Linear, orbital, double orbital
Software Gen5 Microplate Reader and Imager Software included Gen5 Microplate Reader and Imager Software included Gen5™ Microplate Reader and Imager Software included Gen5™ Microplate Reader and Imager Software Gen5™ Microplate Reader and Imager Software
         
Gen5 Image+ and Image Prime Software available for advanced image analysis (option) Gen5 Image+ and Image Prime Software available for advanced image analysis (option) Gen5 Secure for 21 CFR Part 11 compliance (option) Gen5 Image+ and Image Prime software available for full image analysis Gen5 Image+ and Image Prime software available for full image analysis
         
Gen5 Secure for 21 CFR Part 11 Compliance (option) Gen5 Secure for 21 CFR Part 11 Compliance (option)     Gen5 Secure available for 21 CFR Part 11
      Gen5 Secure for 21 CFR Part 11 compliance (option)  
Automation     BioStack and 3rd party automation compatible BioStack and 3rd party automation compatible BioStack and 3rd party automation compatible
     
BioSpa 8 Automated Incubator compatible BioSpa 8 Automated Incubator compatible BioSpa 8 Automated Incubator compatible
CO2 and O2 control (option) 0 - 20% CO2 control and 1 - 19% O2 control, with optional Gas Controller   Range: 0 - 20% (CO2); 1 - 19% (O2) Range: 0 - 20% (CO2); 1 - 19% (O2) 0 - 20% CO2 control and 1 – 19% O2 control, with optional Gas Controller
     
Models for both CO2 and O2 or CO2 only are available Models for both CO2 and O2 or CO2 only are available Models for both CO2 and O2 or CO2 only are available
Environmental control cover Top cover for light tight imaging and incubation control (option)        
X/Y Stage resolution Lead screw driven stage with 0.1 µm resolution Lead screw driven stage with 0.1 micron resolution      
Humidity control Humidity chamber with rapid gas recharge (option)        
Imaging - Inverted Microscope          
Imaging mode Fluorescence, brightfield, high contrast brightfield, color brightfield and phase contrast Fluorescence, high contrast brightfield and color brightfield Fluorescence, color brightfield, user-selectable brightfield/high contrast brightfield Fluorescence, brightfield, high contrast brightfield, color brightfield and phase contrast Fluorescence and high contrast brightfield (2.5x Zeiss, 4x, 10x, 20x, 40x and 60x)
Imaging method Single color, multi-color, montage, time lapse, Z-stacking, burst mode Single color, multi-color, montage, time lapse, Z-stack, z-stack montage, burst mode Single color, multi-color, time lapse, montage, z-stacking, z-stack montage Single color, multi-color, montage, time lapse, z-stacking Single color, multi-color, montage, time lapse, z-stacking
Light source High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm) High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm,      
590 nm, 623 nm, 655 nm, 740 nm)
Image processing     Z-projection, digital phase contrast, stitching Z-projection, digital phase contrast, stitching Z-projection, image stitching
Camera 16-bit grayscale Sony CMOS 16-bit grayscale Sony CMOS 16-bit Sony CMOS wide field of view monochrome camera 16-bit Sony CMOS, standard or wide FOV 16-bit gray scale, Sony
Camera binning Optional 2x2 binning for focus and/or image capture Optional 2x2 binning for focus and/or image capture      
Camera exposure range 5 milliseconds to 4 seconds 5 milliseconds to 4 seconds      
Image outputs available Raw Images: 16-bit TIFF Raw Images: 16-bit TIFF      
   
Saved Images: TIF, JPG, BMP, PNG, EMF, GIF Saved Images: TIFF, JPG, BMP, PNG, EMF, GIF, color TIFF
   
Movies: MP4, WMV Movies: MP4, WMV
Objective capacity 6 onboard, user-replaceable objectives 6 onboard, user-replaceable objectives 6-position automated turret for user-replaceable objectives 6-position automated turret for user-replaceable objectives 2 user-replaceable objectives
Objectives available Fluorescence: Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA.60; 60x, NA .70 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x
   
Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30; Oil: 60x, NA:1.42; 60x, NA 1.25; 100x, NA 1.4; 100x, NA 1.3
   
20x, NA: .45; 40x, NA.60; 60x, NA .70 High NA: 20x, NA 0.75; 40x, NA 0.95
   
Oil: 60x, NA:1.42; 100x, NA: 1.40  
   
Phase objectives available: 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA .60  
Phase objectives available       4x, 10x, 20x, 40x  
Image filter cube capacity 4 user-replaceable fluorescence cubes plus brightfield channel 4 user-replaceable fluorescence cubes plus brightfield channel 4 user-replaceable fluorescence cubes, plus brightfield channel 4 user-replaceable fluorescence cubes plus brightfield channel 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647 DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647 DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-FRET, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, Tag BFP-FRET, GFP (Ex)-CY5 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex 377/Em 647, Oxidized roGFP2, TRITC DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647 DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647
Imaging LED cubes available     365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 554 nm, 590 nm, 623 nm, 655 nm, 740 nm 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions Autofocus, user-trained autofocus, auto exposure, auto-LED intensity Autofocus, user-trained autofocus, auto exposure, auto-LED intensity Autofocus, user-trained autofocus, autoexposure, auto-LED intensity Autofocus, auto LED intensity, auto exposure Autofocus, auto LED intensity, auto exposure
Autofocus method Image-based autofocus Image-based autofocus Image-based autofocus Image-based autofocus Image-based autofocus
         
Laser autofocus option Laser autofocus option User-trained autofocus User-trained autofocus User-trained autofocus
         
    Laser autofocus (option) Laser autofocus (option) Laser autofocus (option)
Positional controls     Software Control Software control Software control
   
Joystick controller (option) Joystick control (option)
Image collection rate Single well fastest frame rate capture: Single well fastest frame rate capture: Image-based autofocus: Image-based autofocus: Image-based autofocus:
         
*Full Resolution: up to 10 frames per second for single color images *Full Resolution: up to 10 frames per second for single color images 96 wells, 1 color (DAPI), 4x, 6 minutes 96 wells, 1 color (DAPI), 4x, 6 minutes 96 wells, 1 color (DAPI), 4x, 6 minutes
         
*2x2 Binning: up to 20 frames per second for single color images *2x2 Binning: up to 20 frames per second for single color images   96 wells, 3 colors, 4x, 12 minutes 96 wells, 3 colors, 4x, 12 minutes
         
    Laser autofocus:    
      Laser autofocus:  
    96 wells, 1 color (DAPI), 4x, <3 minutes   Laser autofocus:
      96 wells, 1 color (DAPI), 4x, <3 minutes  
        96 wells, 1 color (DAPI), 4x, <3 minutes
      96 wells, 3 colors, 4x, <7 minutes, 30 seconds  
        96 wells, 3 colors, 4x, <7 minutes, 30 seconds
      Burst Mode: 10 fps, single well, single color at <= 5Oms integration time  
        Burst Mode: 10 fps, single well, single color at <= 50ms integration time
Image Analysis Software option     Gen5 Image+: Image analysis Gen5 Image+: Image analysis Gen5 Image+: Image analysis
     
Gen5 Image Prime: Advanced image analysis Gen5 Image Prime: Advanced image analysis Gen5 Image Prime: Advanced image analysis
     
Gen5 Secure: 21 CFR Part 11 compliant features Gen5 Secure: 21 CFR Part 11 compliant features Gen5 Secure: 21 CFR Part 11 compliant features
Microscope stage control Gen5 Software Control Gen5 Software Control      
   
Optional joystick controller Optional joystick controller
Imaging - Upright Microscope          
Imaging modes     Reflected color brightfield; transmitted color brightfield    
Imaging methods     Single image, montage, time lapse, Z-stacking    
Image processing     Z-projection, digital phase contrast, stitching    
Camera     16-bit Sony CMOS wide field of view color camera    
Lenses     Finder scope, 2x, 4x, 8x    
Positional controls     Software Control    
 
Joystick controller (option)
Image collection rate     Entire 100 mm dish @ 1x: <2:40 minutes    
 
Entire microscope slide @1x: <1:15 minutes
 
96-well ELISpot plate@ 1x: <5 minutes
Image Analysis Software option     Gen5 Image+: Image analysis    
 
Gen5 Image Prime: Advanced image analysis
 
Gen5 Secure: 21 CFR Part 11 compliant features
Fluorescence Intensity          
Light source     Xenon flash Xenon flash Xenon flash
Detector     PMT (red-shifted PMT option) PMT for monochromator system PMT
 
PMT for filter system
Wavelength selection     Quad monochromators (top/bottom) Quad monochromators (top/bottom) Deep blocking band pass filters / dichroic mirrors
 
Filters (top)
Wavelength range     250 – 700 (900 nm option) Monochromators: 250 - 700 nm (900 nm option) Filters: 200 - 700 nm (850 nm option)
 
Filters: 200 - 700 nm (850 nm option)
Monochromator bandwidth     Variable, from 9 nm to 50 nm in 1 nm increments Variable, from 9 nm to 50 nm in 1 nm increments  
Dynamic range     7 decades 7 decades 7 decades
Sensitivity     Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) – top Filters: Fluorescein: 0.25 pM (0.025 fmol/well, 384-well plate)
   
Fluorescein 4 pM (0.4 fmol/well, 384-well plate) – bottom Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate)
   
   
  Quad Monochromator:
   
  Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) - top
   
  Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom
Reading speed (kinetic)     96 wells: 11 seconds 96 wells: 11 seconds 96 wells: 11 seconds
     
384 wells: 22 seconds 384 wells: 22 seconds 384 wells: 22 seconds
Luminescence          
Wavelength range     300 - 700 nm 300 - 700 nm 300 - 700 nm
Dynamic range     >6 decades >6 decades >6 decades
Sensitivity     20 amol ATP (flash) Monos: 20 amol ATP (flash) 10 amol ATP (flash)
   
Filters: 10 amol ATP (flash), 100 amol (glow) 100 amol (glow)
Fluorescence Polarization          
Light source       Xenon flash Xenon flash
Detector       PMT PMT
Wavelength selection       Filters Filters
Wavelength range       280 - 700 nm (850 nm option) 400 nm – 700 nm
Sensitivity       1.2 mP standard deviation at 1 nM fluorescein 1.2 mP standard deviation at 1 nM fluorescein
Time-Resolved Fluorescence          
Light source       Xenon flash Xenon flash
Detector       PMT PMT
Wavelength selection       Quad monochromators (secondary mode) Filters
 
Filters (top)
Wavelength range       Filters: 200 - 700 nm (850 nm option)  
Sensitivity       Filters: Europium 40 fM (4 amol/well, 384-well plate) Europium 40 fM (4 amol/well, 384-well plate)
 
Monos: Europium 1200 fM (120 amol/well, 384-well plate)
Absorbance          
Light source     Xenon flash Xenon flash Xenon flash
Detector     Photodiode photodiode photodiode
Wavelength selection     Monochromator Monochromator Monochromator
Wavelength range     230 - 999 nm, 1 nm increment 230 - 999 nm, 1 nm increment 200 – 999 nm, 1 nm increment
Monochromator bandwidth     4 nm (230 - 285 nm), 8 nm (>285 nm) 4 nm (230 - 285 nm), 8 nm (>285 nm) 2.4 nm
Dynamic range     0 - 4.0 OD 0 - 4.0 OD 0 – 4.0 OD
Resolution     0.0001 OD 0.0001 OD 0.0001 OD
Pathlength correction     Yes yes yes
Monochromator wavelength accuracy     +2 nm +2 nm + 2 nm
Monochromator wavelength repeatability     +0.2 nm +0.2 nm + 0.2 nm
OD accuracy     <1% at 3.0 OD <1% at 2.0 OD <1% at 2.0 OD
   
<3% at 3.0 OD <3% at 3.0 OD
OD linearity     <1% from 0 to 3.0 OD <1% from 0 to 3.0 OD <1% from 0 to 3.0 OD
OD repeatability     <0.5% at 2.0 OD <0.5% at 2.0 OD <0.5% at 2.0 OD
Stray light     0.03% at 230 nm 0.03% at 230 nm 0.03% at 230 nm
Reading speed (kinetic)     96 wells: 11 seconds 96 wells: 11 seconds 96 wells: 11 seconds
     
384 wells: 22 seconds 384 wells: 22 seconds 384 wells: 22 seconds
Alpha Detection          
Light source       100 mW 680 nm laser  
Detector       PMT  
Wavelength selection       Filters (top)  
Sensitivity       100 amol LCK peptide (384-well low volume plate)  
Reagent Injectors (option)          
Supported detection modes       All modes All modes
Number 2 syringe pumps   2 syringe pumps 2 syringe pumps 2 syringe pumps
Supported labware 6- to 384-well microplates, Petri and cell culture dishes, chamber slides   6- to 384-well plates, Petri and cell culture dishes 6- to 384-well plates, Petri and cell culture dishes 6- to 384-well plates, Petri and cell culture dishes
Dead volume <1.65 mL with back flush   1.1 mL with back flush 1.1 mL with back flush 1.1 mL with back flush
Dispense tip options Aligned tip - aligned with optical path for dispensing for fast kinetic assays        
 
Offset tip - dispensing is offset from the optical path
Dispense volume 5 - 1000 µL in 1 µL increments   5 - 1000 µL in 1 µL increments 5 - 1000 µL in 1 µL increments 5 - 1000 µL in 1 µL increments
Plate geometry     6- to 384-well microplates 6- to 384-well microplates 6- to 384-well microplates
Dispense accuracy +1 µL or 2%   +1 µL or 2% +1 µL or 2% +1 µL or 2%
Dispense precision <2% at 50 - 200 µL   <2% at 50 - 200 µL <2% at 50 - 200 µL <2% at 50 – 200 µL
Physical Characteristics          
Power 250 Watts maximum consumption. 60 Watts maximum consumption. 150 Watts maximum consumption 250 Watts maximum consumption. 100-240 VAC, 50/60 Hz (24VDC external power supply, 150W min)
Dimensions With cover closed or without cover: 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm) 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm) 20.2" D x 16.4" W x 17.5" H 20.2" D x 16.4" W x 17.5" H 20.2" D x 16.4" W x 17.5" H
       
With cover fully open: 18.3" D, 17.9" W, 27.5" H (46.5 cm x 45.5 cm x 69.8 cm) (51.4 cm D x 41.6 cm W x 44.5 cm H) (51.4 cm D x 41.6 cm W x 44.5 cm H) (51.4 cm D x 41.6 cm W x 44.5 cm H)
Weight Without environmental control cover: 51 lbs (23.1 kg) 51 lbs (23.1 kg) 80 lbs (36.3 kg) 80 lbs (36.3 kg) 65 lbs (29 Kg)
 
With environmental control cover: 58 lbs (26.3 kg)
Regulatory          
Regulatory CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available. CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available. CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available. CE and TUV marked. RoHS Compliant. Models for In Vitro CE and TUV marked. Models for In Vitro Diagnostic use are available.
Diagnostic use are available.