CEM Liberty Blue

Synthesize peptides more efficiently than ever before.


The Liberty Blue™ Automated Microwave Peptide Synthesizer is the gold standard for peptide synthesis. It features unmatched 4-minute cycle times along with a 90% solvent reduction based on High Efficiency Solid Phase Peptide synthesis (HE-SPPS) developed in 2013. This system is utilized in hundreds of laboratories worldwide and provides unparalleled peptide quality based on its unique methodology and use of microwave energy. The Liberty Blue features the latest in engineering for fluidic deliveries, true internal temperature feedback control and software control with 21CFR part 11 compliance.

Unique Chemistry
• 4 min cycle time
• 90% waste reduction (HE-SPPS)
• CarboMAX™ coupling chemistry (patented)
• Microwave deprotection and coupling (patented)

• Flex-Add™ critical reagent delivery system (patented)
• True Internal fiber-optic temperature control
• 0.005 - 5 mmol scale range
• 27 amino acid positions
• Integrated Camera
• High-throughput upgrade options

Liberty Blue Product Video

CEM Liberty Blue Details

CEM is transforming the way chemists perform peptide synthesis once again with the introduction of the Liberty Blue™ Microwave Peptide Synthesizer. More than just a face-lift, the Liberty Blue System has been designed for maximized speed and efficiency. With a 4-minute cycle time and a 90% reduction in solvent consumption, no other peptide synthesizer on the market can even come close to the performance of the Liberty Blue. Unparalleled peptide quality, greater flexibility and improved reliability make Liberty Blue the system of choice for all your peptide synthesis needs.

Remarkably fast:
• Rapid reagent addition
• Optimized synthesis methods
• More than 80% time reduction compared to 1st generation microwave synthesizers
• Difficult peptides such as A-beta in hours rather than days
The 4-minute cycle time of the Liberty Blue System is not limited to easily synthesized peptides. These methods can be used to routinely synthesize a wide range of peptides. Using the standard Liberty Blue methods, A-beta 1-42 was prepared in less than 4 hours in 68% crude purity.

Maximum efficiency:
• Up to 90% solvent reduction
• Less reagent usage
• No priming required
• Judicious use of solvent for washing
• Accurate and precise reagent addition
Liberty Blue offers several advantages over alternative heating methods. The microwave energy can be controlled for each step, and the reaction temperature can be carefully selected depending on the specific peptide and/or reagents used.
CEM is the only microwave provider than can apply the microwave energy to the entire process. Microwave irradiation heats the reaction from the inside rather than the outside making it possible to rapidly change the temperature from one stage to the next. This can be especially important when synthesizing peptides containing temperature-sensitive amino acids, such as phosphopeptides, or peptides containing difficult to couple residues such as Aib.
The synthesis of the double Aib containing peptide below was performed with the coupling of the second Aib residue at both 75 °C and at 95 °C with all of the rest of the conditions the same. The coupling at 75 °C resulted in only 44% crude purity with a significant Aib deletion while the 95 °C gave 90% crude purity.

Peptide Sequence: GEQKLGAib*AibASEEDLG-NH2
peptide sequence

• Flex-Add™ technology eliminates need for sample loops and allows infinite volume delivery options
• Synthesis conditions (temperature, power, and time) can be controlled for each step of every amino acid cycle
• 27 AA positions provide ample capacity for unusual amino acids or reagents
• Unlimited number of couplings with amino acid stock solutions
• 2 reaction vessel sizes (30-, and 125-mL) to cover the full synthesis scale range
• Scale range from 0.005 to 5 mmollResin loading option allows high-throughput peptide synthesis
• Optional cleavage accessory for rapid peptide cleavage and deprotection
Synthesis Scale 0.005 – 5 mmol
Reaction Vessel Sizes 30- and 125-mL standard
Chemistry Fmoc only
Activation Method In situ, standard activators
Amino Acid Reagents 20, plus 7 additional positions
External Bottle Positions 2, plus 7 additional positions
Temperature Measurement In situ fiber-optic
Fluid Measurement Flex-Add – amino acids, activator, activator base
Timed delivery – Main wash, Deprotection
Agitation Programmable inert gas bubbling
Waste Container 1 L reservoir with overflow detection
Larger sizes available
Controller Laptop with wireless option standard
Optional Features Resin Loader for high throughput
Cleavage module
Reports Printable .pdf file for each method created. Log created during each run of every step performed
Power 120V/60Hz or 240V/50Hz
Dimensions 20”W x 18”D x 30”H
(51 cm x 46 cm x 76 cm)
License The purchase of this instrument includes a grant of a limited, non-transferrable license to utilize this instrument for microwave peptide synthesis using microwave energy for both the coupling and deprotection steps.
Patents US7393920; US7582728; US8058393; EP1491552; JP4773695, with additional worldwide patents pending