BioTek Lionheart FX

Automated Microscope

Overview

Lionheart™ FX Automated Microscope is a compact, inclusive microscopy system for a broad range of imaging workflows. It offers up to 100x air and oil immersion magnification, with fluorescence, brightfield, color brightfield, and phase contrast channels for maximum application reach. An optional environment control cover provides incubation to 40°C and effective containment for CO2/O2 control, a humidity chamber optimizes conditions for long-term live cell imaging applications, and an available dual reagent injector facilitates rapid kinetic assays. Lionheart FX and Gen5 together comprise Augmented Microscopy™ - the automation of image capture, processing, analysis and development of publication-ready images and data.

• Automated digital microscopy up to 100x
• Fluorescence, brightfield, color brightfield and phase contrast imaging
• Label-free cell counting for cell proliferation studies
• Live cell assay support with environmental controls
• Compact design for quick installation and setup

Lionheart FX product video.

BioTek Lionheart FX Details



Automated digital microscopy to 100x.
Lionheart FX with Augmented Microscopy automates make your imaging workflow to yield amazing images and powerful data. Automated image capture starts with image-based and laser autofocus, plus auto LED and exposure. Automated image pre-processing optimizes images for downstream analysis, from cell counting to characterization of subcellular details.

Four imaging modes, multiple imaging processes
With brightfield, color brightfield, phase contrast and fluorescence imaging in four channels – and nearly twenty available filter/LED cubes, Lionheart FX offers fantastic versatility that isn’t limited to image capture. Follow your imaging processes through z-stacking to z-projection, montage collection to stitching and digital phase contrast to analysis, all one hardware and software platform.

Label-free cell counting
It’s fast and simple to count live cells using the available high contrast brightfield option. Higher contrast allows a direct and label-free method for object identification using Gen5 software to count cells and characterize cell proliferation in kinetic studies.

Kinetic live cell assay support
Several options are available to support live cell assays with Lionheart FX, including an environmental cover to maintain temperature and gas levels within the system. Long-term live cell kinetics are supported, using the unique humidity chamber that fits on the stage itself, keeping the live cell in a completely nurturing environment. A dual reagent injector provides quick inject/image capability for observing fast reactions.

Fully integrated and compact
Fifteen minutes is all it takes to install and set up Lionheart FX for image capture and analysis. The filter/LED cubes and objectives are accessed from the front panel, and tool-free connections for injectors, gas control and the environmental cover add to the easy installation. Its small size takes up minimal benchtop space.

GENERAL
Microplate types 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides
Support for labware up to 1.5" tall
Temperature control Incubation to 40 °C with optional environmental control cover
Software Gen5 Microplate Reader and Imager Software included
Gen5 Image and Image+ Software available for advanced image analysis (option)
CO2 and O2 control (option) 0 - 20% CO2 control and 1 - 19% O2 control, with optional Gas Controller
Environmental control cover Top cover for light tight imaging and incubation control (option)
X/Y Stage resolution Lead screw driven stage with 0.1 micron resolution
Humidity control Humidity chamber with rapid gas recharge (option)
IMAGING SYSTEM
Imaging mode Fluorescence, brightfield, color brightfield, and phase contrast
Imaging method Single color, multi-color, montage, time lapse, Z-stacking, burst mode
Light source High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm)
Camera 16-bit gray scale, Sony CCD, 1.25 megapixel
Camera binning Optional 2x2 binning for focus and/or image capture
Camera exposure range 5 milliseconds to 4 seconds
Image outputs available Raw Images: 16-bit TIFF
Saved Images: TIF, JPG, BMP, PNG, EMF, GIF
Movies: MP4, WMV
Objective capacity 6 onboard, user-replaceable objectives
Objectives available Fluorescence: 
Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30; 
20x, NA: .45; 40x, NA.60; 60x, NA .70 
Oil: 60x, NA:1.42; 100x, NA: 1.40
Phase objectives available: 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA .60
Image filter cube capacity 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647
Automated functions Autofocus, user-trained autofocus, auto exposure, auto-LED intensity
Autofocus method Image-based autofocus
Laser autofocus option
Image collection rate Single well fastest frame rate capture:
*Full Resolution: up to 10 frames per second for single color images
*2x2 Binning: up to 20 frames per second for single color images
Microscope stage control Gen5 Software Control
Optional joystick controller
REAGENT INJECTORS (OPTION)
Number 2 syringe pumps
Supported labware 6- to 384-well microplates, Petri and cell culture dishes, chamber slides
Dead volume <1.65 mL with back flush
Dispense tip options Aligned tip - aligned with optical path for dispensing for fast kinetic assays
Offset tip - dispensing is offset from the optical path
Dispense volume 5 - 1000 µL in 1 µL increments
Dispense accuracy +1 µL or 2%
Dispense precision <2% at 50 - 200 µL
PHYSICAL CHARACTERISTICS
Power 250 Watts max.
Dimensions With cover closed or without cover: 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm)
With cover fully open: 18.3" D, 17.9" W, 27.5" H (46.5 cm x 45.5 cm x 69.8 cm)
Weight Without environmental control cover: 51 lbs (23.1 kg)
With environmental control cover: 58 lbs (26.3 kg)
REGULATORY
Regulatory CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available.
Gen5


Gen5 Microplate Reader and Imager Software controls Lionheart FX for automated image capture, processing, analysis and development of publication-ready images and data. This imaging and microscopy automation represents BioTek’s Augmented Microscopy™ - the simplified, automated process to accomplish multiple microscopy steps in a single platform. Gen5 is available in several editions to meet the image analysis requirements across a range of applications.

Gen5 Image+ offers complete imaging control for Lionheart FX Automated Microscope, and provides image processing and analysis including z-projection, image stitching, hitpicking and digital phase contrast.

Gen5 Image Prime controls Lionheart FX Automated Microscope includes more expansive image processing and analysis: Z-projection, image stitching, hitpicking and digital phase contrast, plus advanced features including secondary mask, dynamic thresholding and subpopulation analysis.

Gen5 Secure editions add features to help ensure compliance to 21 CFR Part 11 requirements. Gen5 add-on modules provide increased functionality for specific applications or analyses, such as Spot Counting.
LFX
Lionheart FX Automated Live Cell Imager. Fluorescence, brightfield, color brightfield and phase contrast imaging capabililty. Includes base Gen5 software. Objectives, filter/LED cubes, accessories and software upgrades sold separately.
  Lionheart FX Lionheart LX
     
General    
Microplate types 6- to 1536-well plates 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides
     
  Support for labware up to 1.5" tall Support for labware up to 1.5" tall
Temperature control Incubation to 40 °C with optional environmental control cover  
Software Gen5 Microplate Reader and Imager Software included Gen5 Microplate Reader and Imager Software included
     
  Gen5 Image and Image+ Software available for advanced image analysis (option) Gen5 Image and Image+ Software available for advanced image analysis (option)
CO2 and O2 control (option) 0 - 20% CO2 control and 1 - 19% O2 control, with optional Gas Controller  
Environmental control cover Top cover for light tight imaging and incubation control (option)  
X/Y Stage resolution Lead screw driven stage with 0.1 micron resolution Lead screw driven stage with 0.1 micron resolution
Humidity control Humidity chamber with rapid gas recharge (option)  
Physical Characteristics    
Power 250 Watts max. 250 Watts max.
Dimensions With cover closed or without cover: 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm) 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm)
     
  With cover fully open: 18.3" D, 17.9" W, 27.5" H (46.5 cm x 45.5 cm x 69.8 cm)  
Weight Without environmental control cover: 51 lbs (23.1 kg) 51 lbs (23.1 kg)
     
  With environmental control cover: 58 lbs (26.3 kg)  
Regulatory    
Regulatory CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available. CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available.
Imaging System    
Imaging mode Fluorescence, brightfield, color brightfield, and phase contrast Fluorescence, high contrast brightfield and color brightfield
Imaging method Single color, multi-color, montage, time lapse, Z-stacking, burst mode Single color, multi-color, montage, time lapse, Z-stack, z-stack montage, burst mode
Light source High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm) High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 
    590 nm, 623 nm, 655 nm, 740 nm)
Camera 16-bit gray scale, Sony CCD, 1.25 megapixel 16-bit CMOS camera. 2.3 MP Sony IMX249 1/1.2" sensor with 5.86 micron pixel size
Camera binning Optional 2x2 binning for focus and/or image capture Optional 2x2 binning for focus and/or image capture
Camera exposure range 5 milliseconds to 4 seconds 5 milliseconds to 4 seconds
Image outputs available Raw Images: 16-bit TIFF Raw Images: 16-bit TIFF
     
  Saved Images: TIF, JPG, BMP, PNG, EMF, GIF Saved Images: TIFF, JPG, BMP, PNG, EMF, GIF, color TIFF
     
  Movies: MP4, WMV Movies: MP4, WMV
Objective capacity 6 onboard, user-replaceable objectives 6 onboard, user-replaceable objectives
Objectives available Fluorescence:  Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA.60; 60x, NA .70
     
  Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30;  Oil: 60x, NA:1.42; 60x, NA 1.25; 100x, NA 1.4; 100x, NA 1.3
     
  20x, NA: .45; 40x, NA.60; 60x, NA .70  High NA: 20x, NA 0.75; 40x, NA 0.95
     
  Oil: 60x, NA:1.42; 100x, NA: 1.40  
     
  Phase objectives available: 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA .60  
Image filter cube capacity 4 user-replaceable fluorescence cubes plus brightfield channel 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647 DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647
Automated functions Autofocus, user-trained autofocus, auto exposure, auto-LED intensity Autofocus, user-trained autofocus, auto exposure, auto-LED intensity
Autofocus method Image-based autofocus Image-based autofocus
     
  Laser autofocus option Laser autofocus option
Image collection rate Single well fastest frame rate capture: Single well fastest frame rate capture:
     
  *Full Resolution: up to 10 frames per second for single color images *Full Resolution: up to 10 frames per second for single color images
     
  *2x2 Binning: up to 20 frames per second for single color images *2x2 Binning: up to 20 frames per second for single color images
Microscope stage control Gen5 Software Control Gen5 Software Control
     
  Optional joystick controller Optional joystick controller
Reagent Injectors (option)    
Number 2 syringe pumps  
Supported labware 6- to 384-well microplates, Petri and cell culture dishes, chamber slides  
Dead volume <1.65 mL with back flush  
Dispense tip options Aligned tip - aligned with optical path for dispensing for fast kinetic assays  
     
  Offset tip - dispensing is offset from the optical path  
Dispense volume 5 - 1000 µL in 1 µL increments  
Dispense accuracy +1 µL or 2%  
Dispense precision <2% at 50 - 200 µL  
Specifications are subject to change.