BioTek Lionheart LX

Automated Microscope

Overview

BioTek’s new Lionheart LX Automated Microscope is designed with affordability in mind, offering an alternative to expensive, custom-built automated microscopes that are complex to learn and use. The compact, ocular-free hardware design eases ergonomic discomfort. Along with Gen5™ software, Lionheart LX enables Augmented Microscopy™, which fully automates image capture, processing and analysis. Load samples, start a run and come back to publication-ready images and quantitative data. Lionheart LX can help increase your research output while reducing processing time and costs.

• Affordable automated digital microscopy
• High contrast brightfield, color brightfield and fluorescence imaging
• Augmented Microscopy: Easy imaging workflow automation
• Quick analyze: instant cell count and confluence measurement
• Integrated, compact design offers quick installation and setup

Lionheart FX product video.

BioTek Lionheart LX Details



Affordable automated digital microscopy.
Lionheart LX has an automated 6-objective turret and can contain up to 4 LED/filter cubes for multi-channel image capture. The precise automated stage and autofocus, auto exposure and other Gen5 software features enable simple, automated image capture and analysis.

High contrast brightfield, color brightfield and fluorescence imaging
With brightfield, color brightfield and fluorescence imaging in four channels – and more than twenty available filter/LED cubes, Lionheart LX offers fantastic versatility that isn’t limited to image capture. Imaging processes from z-stacking to z-projection, montage collection to stitching; plus analysis from label-free cell counting to mitochrondrial membrane potential are all accomplished with Lionheart LX and Gen5 software.

Augmented Microscopy: Easy imaging workflow automation
BioTek’s Augmented Microscopy automates image capture, processing and analysis for publication-ready images and data. Automated image capture starts with image-based and laser autofocus, plus auto LED and exposure. Automated image pre-processing optimizes images for downstream analysis, from cell counting to characterization of subcellular details

Quick analyze: instant cell count and confluence measurement
Get cell counts and confluence calculations directly on the live camera feed. Gen5 software controls Lionheart LX to quickly find the region of interest and display counts onscreen without needing to capture the image. The quick analyze function works for samples in a variety of labware.

Integrated, compact design offers quick installation and setup
Lionheart LX integrates all microscopy hardware into a very compact footprint, saving valuable bench space. The easily accessible objectives and LED/filter cubes make setup and operation fast and simple, with a minimal learning curve.

GENERAL
Microplate types 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides
Support for labware up to 1.5" tall
Software Gen5 Microplate Reader and Imager Software included
Gen5 Image and Image+ Software available for advanced image analysis (option)
X/Y Stage resolution Lead screw driven stage with 0.1 micron resolution
IMAGING SYSTEM
Imaging mode Fluorescence, high contrast brightfield and color brightfield
Imaging method Single color, multi-color, montage, time lapse, Z-stack, z-stack montage, burst mode
Light source High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm)
Camera 16-bit CMOS camera. 2.3 MP Sony IMX249 1/1.2" sensor with 5.86 micron pixel size
Camera binning Optional 2x2 binning for focus and/or image capture
Camera exposure range 5 milliseconds to 4 seconds
Image outputs available Raw Images: 16-bit TIFF
Saved Images: TIFF, JPG, BMP, PNG, EMF, GIF, color TIFF
Movies: MP4, WMV
Objective capacity 6 onboard, user-replaceable objectives
Objectives available Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA.60; 60x, NA .70
Oil: 60x, NA:1.42; 60x, NA 1.25; 100x, NA 1.4; 100x, NA 1.3
High NA: 20x, NA 0.75; 40x, NA 0.95
Image filter cube capacity 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647
Automated functions Autofocus, user-trained autofocus, auto exposure, auto-LED intensity
Autofocus method Image-based autofocus
Laser autofocus option
Image collection rate Single well fastest frame rate capture:
*Full Resolution: up to 10 frames per second for single color images
*2x2 Binning: up to 20 frames per second for single color images
Microscope stage control Gen5 Software Control
Optional joystick controller
PHYSICAL CHARACTERISTICS
Power 250 Watts max.
Dimensions 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm)
Weight 51 lbs (23.1 kg)
REGULATORY
Regulatory CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available.
Gen5


Gen5 Microplate Reader and Imager Software controls Lionheart LX for automated image capture, processing, analysis and development of publication-ready images and data. This imaging and microscopy automation represents BioTek’s Augmented Microscopy™ - the simplified, automated process to accomplish multiple microscopy steps in a single platform. Gen5 is available in several editions to meet the image analysis requirements across a range of applications.

Gen5 Image+ offers complete imaging control for Lionheart LX Automated Microscope, and provides image processing and analysis including z-projection, image stitching, hitpicking and digital phase contrast.

Gen5 Image Prime controls Lionheart LX Automated Microscope includes more expansive image processing and analysis: Z-projection, image stitching, hitpicking and digital phase contrast, plus advanced features including secondary mask, dynamic thresholding and subpopulation analysis.

Gen5 Secure editions add features to help ensure compliance to 21 CFR Part 11 requirements.

Gen5 add-on modules provide increased functionality for specific applications or analyses, such as Spot Counting.
LLX
Lionheart LX Automated Microscope. Fluorescence, high contrast brightfield and color brightfield imaging. Includes standard Gen5 software and 4-position slide holder. The imaging controller, objectives, filter/LED cubes, accessories and software upgrades are sold separately.
  Lionheart FX Lionheart LX
     
General    
Microplate types 6- to 1536-well plates 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides
     
  Support for labware up to 1.5" tall Support for labware up to 1.5" tall
Temperature control Incubation to 40 °C with optional environmental control cover  
Software Gen5 Microplate Reader and Imager Software included Gen5 Microplate Reader and Imager Software included
     
  Gen5 Image and Image+ Software available for advanced image analysis (option) Gen5 Image and Image+ Software available for advanced image analysis (option)
CO2 and O2 control (option) 0 - 20% CO2 control and 1 - 19% O2 control, with optional Gas Controller  
Environmental control cover Top cover for light tight imaging and incubation control (option)  
X/Y Stage resolution Lead screw driven stage with 0.1 micron resolution Lead screw driven stage with 0.1 micron resolution
Humidity control Humidity chamber with rapid gas recharge (option)  
Physical Characteristics    
Power 250 Watts max. 250 Watts max.
Dimensions With cover closed or without cover: 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm) 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm)
     
  With cover fully open: 18.3" D, 17.9" W, 27.5" H (46.5 cm x 45.5 cm x 69.8 cm)  
Weight Without environmental control cover: 51 lbs (23.1 kg) 51 lbs (23.1 kg)
     
  With environmental control cover: 58 lbs (26.3 kg)  
Regulatory    
Regulatory CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available. CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available.
Imaging System    
Imaging mode Fluorescence, brightfield, color brightfield, and phase contrast Fluorescence, high contrast brightfield and color brightfield
Imaging method Single color, multi-color, montage, time lapse, Z-stacking, burst mode Single color, multi-color, montage, time lapse, Z-stack, z-stack montage, burst mode
Light source High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm) High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 
    590 nm, 623 nm, 655 nm, 740 nm)
Camera 16-bit gray scale, Sony CCD, 1.25 megapixel 16-bit CMOS camera. 2.3 MP Sony IMX249 1/1.2" sensor with 5.86 micron pixel size
Camera binning Optional 2x2 binning for focus and/or image capture Optional 2x2 binning for focus and/or image capture
Camera exposure range 5 milliseconds to 4 seconds 5 milliseconds to 4 seconds
Image outputs available Raw Images: 16-bit TIFF Raw Images: 16-bit TIFF
     
  Saved Images: TIF, JPG, BMP, PNG, EMF, GIF Saved Images: TIFF, JPG, BMP, PNG, EMF, GIF, color TIFF
     
  Movies: MP4, WMV Movies: MP4, WMV
Objective capacity 6 onboard, user-replaceable objectives 6 onboard, user-replaceable objectives
Objectives available Fluorescence:  Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA.60; 60x, NA .70
     
  Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30;  Oil: 60x, NA:1.42; 60x, NA 1.25; 100x, NA 1.4; 100x, NA 1.3
     
  20x, NA: .45; 40x, NA.60; 60x, NA .70  High NA: 20x, NA 0.75; 40x, NA 0.95
     
  Oil: 60x, NA:1.42; 100x, NA: 1.40  
     
  Phase objectives available: 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA .60  
Image filter cube capacity 4 user-replaceable fluorescence cubes plus brightfield channel 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647 DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647
Automated functions Autofocus, user-trained autofocus, auto exposure, auto-LED intensity Autofocus, user-trained autofocus, auto exposure, auto-LED intensity
Autofocus method Image-based autofocus Image-based autofocus
     
  Laser autofocus option Laser autofocus option
Image collection rate Single well fastest frame rate capture: Single well fastest frame rate capture:
     
  *Full Resolution: up to 10 frames per second for single color images *Full Resolution: up to 10 frames per second for single color images
     
  *2x2 Binning: up to 20 frames per second for single color images *2x2 Binning: up to 20 frames per second for single color images
Microscope stage control Gen5 Software Control Gen5 Software Control
     
  Optional joystick controller Optional joystick controller
Reagent Injectors (option)    
Number 2 syringe pumps  
Supported labware 6- to 384-well microplates, Petri and cell culture dishes, chamber slides  
Dead volume <1.65 mL with back flush  
Dispense tip options Aligned tip - aligned with optical path for dispensing for fast kinetic assays  
     
  Offset tip - dispensing is offset from the optical path  
Dispense volume 5 - 1000 µL in 1 µL increments  
Dispense accuracy +1 µL or 2%  
Dispense precision <2% at 50 - 200 µL  
Specifications are subject to change.