Agilent NovoSampler Pro & Q

Flow Cytometry Automation

NovoSampler Pro

The NovoSampler Pro is compatible with 24-, 48-, and 96-well plate configurations. The sampler can accommodate 24 tube racks as well. Load your plate and let the system record the data for you.
Walk away convenience delivers on time savings and cost savings in the laboratory. Flexible sampling of microplates allows for small projects or high-throughput, 24/7 workflows. Low carryover, and orbital shaking allow you to maximize your efficiency and make the instrument work for you.

• Trusted results with low carryover – less than 0.1% (volume)
• Convenient automated sample mixing – Orbital shaking up to 3000 rpm
• Versatile workflows are compatible with 24-, 48-, 96-well plate, and 24 tube racks
• Walk away sample acquistion
• Save time by making the instrument work for you

NovoSampler Q

The NovoSampler Q is compatible with 24-, 48-, 96-, 384- well plate configurations. It can accommodate 24 and 40 tube racks as well. Automation tools for sample handling and integrated software deliver on a workflow with minimal manual intervention. Walk away convenience delivers on time savings and cost savings in the laboratory. Flexible sampling of microplates allows for small projects or high-throughput, 24/7 workflows. Low carryover, orbital shaking, and embedded barcode reader ensure that your samples are processed quickly and accurately with results you can trust.

• Trusted results with low carryover – less than 0.1% (volume)
• Convenient automated sample mixing – Orbital shaking up to 3000 rpm
• Rapid results, 96 well plate less than 20 min
• Versatile workflows are compatible with 24/48/96/384-well plate, 24 and 40 tube racks
• Save administrative time in sample tracking with the embedded barcode reader


NovoCyte Agilent NovoSampler Pro & Q

Fasten and simplify your research with ACEA’s NovoSampler: an automatic sample loading system that is compatible with the NovoCyte Flow Cytometers. The NovoSampler Pro is compatible with the NovoCyte Flow Cytometer, while the NovoSampler Q is suitable for the NovoCyte Advanteon, Quanteon, and Penteon Flow Cytometers. Lab friendly and easy to operate is what makes the NovoSampler an beneficial asset for your laboratory throughput.

NovoSampler Pro
• Integrates with the NovoCyte Flow Cytometer.
• Compatible with 24-, 48-, and 96-well plates as well as 24 tube racks.
• Time and cost savings in the laboratory through walk away convenience.
• Delivers high-speed analysis and processing performance.
• Increase productivity while maintaining homogeneity and integrity of the samples.
• Including programmable vortex mechanism.

NovoSampler Q
• Integrates with the NovoCyte Advanteon, Quanteon, and Penteon Flow Cytometers.
• Compatible with 24-, 48-, 96-, and 384-well plates as well as 24 and 40 tube racks.
• Time and cost savings in the laboratory through walk away convenience.
• Minimized sample carryover results in a high-performance analysis.
• Minimal manual intervention allows for small projects or high-throughput, 24/7 workflows.
• Including programmable vortex mechanism and embedded barcode reader.

Agilent Novocyte

Flexibility & Performance
The NovoSampler Q is an automatic sample loading system that fulfills the requirement of high-througput and automated sample acquisition. The NovoSample Q saeamlessly integrates with NovoCyte Advanteon, is exceptionally easy to operate, and delivers high-speed processing and analysis performace.

• Automated plate calibration eliminates the need for manual alignment and calibration.
• Versatile loading modes with a variety sample formats (40 tube rack, 24/48/96/384 well plates), as well as customizable plates.
• Rapid and high-throughput reading, in 20 minutes or faster for a 96-well plate and less than 80 minutes for a 384 well plate.
• Reliable orbital shaking keeps samples in suspension at all times, which is important in dosing experimantes where cell settling would skew results.
• Fully integrated barcode reader provides rapid sample identification and tracking.

novocyte automation

Ensure homogeneity of mixed samples
NovoSampler Pro ensures homogeneity of mixed sample and maintains integrity of biological samples. Cell viability of Jurkat cells stained with Propidium Iodide (PI) are maintained across the entire plate (96-well) during mixing and sampling (Figure 3). Jurkat cells were suspended with PBS containing 0.2% BSA, stained with PI (2 μg/mL), and loaded onto a 96-well plate at 100uL per well. Sample loading settings: default settings, stop condition of 30μL, sample flow rate 66μL/min

novocyte automation

Delivers consistent data across different loading templates
NovoSampler Pro delivers consistent data across different loading templates between the autosampler and individual tubes. Normal human peripheral blood stained with CD45 / CD3 / CD4 / CD8 cocktail was loaded into FACS tubes (Single Tube, 24 Tube Rack) or a 96-well flat-bottom plate (96x Flat). Lymphocyte subpopulations were counted and analyzed after acquisition using the NovoSampler Pro autosampler or single tube. Statistics for each are shown below the plots. Consistent results were obtained among the three methods of sample loading.

novocyte automation

Apoptosis Assay
Apoptosis, or programmed cell death, is the process by which cells regulate how they die, activating specific pathways that cause the cell to shrink, condense, and eventually be cleared by phagocytosis. This is in contrast to necrotic cell death where cells die uncontrollably and fall apart, which can lead to detrimental effects such as the activation of an immune response. Therefore, apoptotic cells that die in a very orderly fashion limit disruption of nearby cells and tissue.
There are many ways to measure cell death and distinguish it from apoptosis or necrosis. These assays are easily quantified using the NovoCyte flow cytometer due to automatic compensation settings and a wide dynamic range of fluorescence detection which eliminates the need for any PMT voltage adjustments.


Immune status is associated with disease state, treatment efficiency, and response to external stimuli such as vaccines. Immunophenotyping quickly identifies candidate cell types, sub-classes and functions. Monitoring the frequency of numerous immune cell population as well as the differentiation/activation status of specific cell subsets such as monocytes, NK cells, T and B cells is essential as they may influence the immunogenicity of a vaccine and its efficiency. The NovoCyte Flow Cytometer enables simultaneous quantification of multiple leukocytes for better understanding the immune status of patients and surveillance of the immune response to infectious disease.

Specifcity Clone Fluorocrome Purpose
CD3 UCHT1 PE-TR (ECD) Lineage T cells
CD4 S3.6 PE-Alexa 700 Lineage T cells
CD8 SK1 PerCP-Cy5.5" Lineage T cells
CD19 J3-129 PerCP-eFluor 710 B cells
CD14 MφP9 BV711 Monocytes
CD56 HCD56 BV605 NK cells and NK T-like cells
CD16 3G8 APC-Cy7 NK cells and monocytes
γδ TCR 11F2 PE-Cy7 γδ T cells
Vγ2 TCR B6 PE γδ T cells
CD25 M-A251 BV421 Tregs
CD127 A019D5 APC Tregs/memory/differentiation
CD45RA HI100 BV650 Memory/differentiation
CCR7 G043H7 BV785 Memory/differentiation
CD57 NK-1 FITC Memory/differentiation
HLA-DR B169414 BV570 Activation
CD38 HIT2 PE-Cy5 Activation/plasmablasts
NKG2C 134591 Alexa 700 NK receptor
Dead Cells 134591 AViD Dead cell exclusion

Intracellular Protein Detection
Detection and analysis of intracellular proteins allow for additional characterization of cell subpopulations and cellular processes. In order to analyze proteins not located on the cell surface, fixation and permeabilization of the cell is required. However, many phospho-specific antibodies are not compatible with many common detergent-based permeabilization methods used for intracellular staining. Special attention is needed when determining the proper fix/perm method for your phospho-specific antibody. The most common method uses 1.5% paraformaldehyde for fixation followed by 100% methanol for permeabilization. While this method works for many antibodies, please note it may not work for every phospho-specific antibody.

Additionally, identifying various cell populations in a heterogenous sample requires staining for phosphorylated proteins coupled with surface proteins. Special consideration must be given to the sensitivity of these epitopes to fixative, taking precaution to avoid damage to the epitope. Therefore, the sample may require staining for specific surface markers before fixation.


Cell Cycle Analysis
Normal human somatic cells are diploids containing a constant amount of DNA. During cell cycle progression, DNA synthesis results in a doubling of total DNA content, followed by restoration of the normal DNA content after mitosis. Detailed cell cycle analysis can be performed to understand tumor cell differentiation, cell transformation and cell-compound interaction with the NovoCyte flow cytometer.


Figure: After treatment with 10 migrograms/M MG132 or 500 micrograms/M 5-FI for 16 hours/ A549 cells were analyzed for cell cycle distribution with the ACEA Novocyte flow cytometer. The the Novoexpress built-in cell cycle analysis module, the plot shows cells ni G0/G1 phase (green), S phase (yellow) and G2/M phase (blue). Compared to normal untreated cells, MG132 treated cells were arrested at G2/M phase, while 5-FU treated cells were arrested at G0/G1 phase.

Cell Proliferation
Cell proliferation is an essential function and highly structured event that when unregulated, can cause disease. We can measure proliferation through absolute cell counts or with a dye, such as CFSE. When cells labeled with CFSE divide, the dye is partitioned equally between daughter cells and we can measure the loss of CFSE fluorescence over time as the dye is continuously diluted. The mean fluorescence intensity (MFI) of the dye was also plotted with cell concentration over time to show the inverse relationship between the two. This type of assay is often used to look at changes in T lymphocyte activation.

application Figure: Measurement of proliferation in Jurkat T cells using CFSA. A) Jurkat T cells were labeled with CFSE and analyzed on the NovoCyte flow cytometer over time to measure cell division. Each peak is representative of an individual time point. B) Absolute cell counts are plotted alongside mean fluorescense intensity (MFI) of CFSE over time showing the dilution of signal as cells divide.


349 nm

405 nm

488 nm

561 nm

637 nm

Maximum Number of Fluorescence Channels
NovoCyte Advanteon** 1









NovoCyte Quanteon** 4

NovoCyte Penteon* 5

* RUO: Research use only. Not for use in diagnostic procedures.
** Selected configurations are registered as CE-IVD.