The 405™ LS Microplate Washer is the fifth generation Agilent-BioTek washer, known for superior performance and reliability for washing protocols ranging from gentle cell washing to vigorous ELISAs. The 405 LS has a simple user interface for creation and execution of wash, dispense, aspirate and maintenance protocols within the extensive onboard software. For use under computer control, BioTek’s LHC™ Software is available in standard and 21 CFR Part 11 compliant versions.
The optional BioStack™ is available for walk-away automation of up to 50 microplates.
Part # |
384-well |
96-well |
Optimized Cell Washing |
4-Buffer Switching |
Ultrasonic Advantage |
Verify™ technology |
Biomagnetic Separation** |
Vacuum Filtration** |
405LSHT |
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405LSHTV |
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405LSU |
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405LSUV |
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405LSUS |
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405LSUSQ |
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405LSUVS |
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405LSUVSQ |
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405LSR |
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405LSRV |
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405LSRS |
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405LSRSQ |
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405LSRVS |
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405LSRVSQ |
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405™ LS Microplate Washer Configurations | ||
Feature | Design | |
HT | Faster 384-well microplate washing | 192-tube (16 x 12) manifold |
Dual-Action™ | ||
HT Includes Ultrasonic Advantage | ||
V | Automated Internal 4-Buffer Switching | |
S | Ultrasonic Advantage™ | Built-in ultrasonic cleaner for manifold self-maintenance |
U | 96-/384-well microplate washing | 96-tube (8 x 12) manifold |
Dual-Action™ | ||
R | 96-well microplate washing | 96-tube (8 x 12) manifold |
Q | Verify™ technology | Built-in clog detection for dispense and aspirate tubes |
GENERAL | |
Microplate types | 96- and 384-well |
Low profile and standard height | |
Solid and filter bottom (option) | |
Onboard software | Create, edit or run multiple protocols |
Software (computer control) | LHC2 pc software |
LHC2 Secure for 21 CFR Part 11 compliance (option) | |
SiLA Compliant driver (option) | |
Separation | Biomagnetic separation, vacuum filtration (optional) |
Shaking | Programmable up to 60 minutes |
Slow, medium, fast or variable | |
Soaking | Programmable up to 60 minutes |
Ultrasonic Advantage™ | Yes (option) |
Verify™ clog detection | Yes (option) |
Automation | BioStack and 3rd party automation compatible |
BioSpa 8 Automated Incubator compatible | |
WASHING | |
Manifold types | 96-well washing: 96-tube manifold – straight dispense tubes |
96- and 384-well washing: 96-tube Dual-Action manifold – 20° angled dispense tubes | |
384-well washing (fast): 192-tube Dual-Action manifold – 7° angled dispense tubes | |
Volume range | 25 to 3,000 µL/well, in 1 µL increments |
Wash cycles | 1 – 250 |
Buffer/reagent selection | Auto switching (internal) for up to 4 buffers (option) |
Supply bottle | 4 L or 10 L (optional) |
Dispense precision | <3% CV: 300 µL/well (96-well washing) |
<4% CV: 80 µL/well (384-well washing) | |
Residual volume | <2 µL/well (96- & 384-well plates) |
96-tube manifold for 96 wells; 192-tube for 384 wells | |
Wash speed | 96-wells, 300 µL/well, 3 cycles; <30 seconds |
384-wells,100 µL/well, 3 cycles: <80 seconds | |
384-wells, 400 µL/well, 1 cycle: <20 seconds | |
Flow rates | High flow to low flow |
Optimized rates for cell assays | |
Sterilization | Chemical |
Vacuum filtration | Selectable: Approximate final at 30 seconds: |
Lowest: -38 mmHg | |
Low: -113 mmHg | |
Medium: -200 mmHg | |
High: -390 mmHg | |
Highest: -506 mmHg | |
Vacuum filtration time range: 5 to 999 seconds | |
PHYSICAL CHARACTERISTICS | |
Power | 100 – 240 Volts AC. 50/60 Hz |
Dimensions | 14″ W x 17″ D x 10″ H |
(35.6 x 43.2 x 25.4 cm) | |
Weight | With internal buffer switching – 36 lbs (16.5 kg) |
Without – 30 lbs (13.5 kg) | |
REGULATORY | |
Regulatory | CE and TUV marked. ROHS compliant. In Vitro Diagnostic use models are available. |
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