BioTek Cytation 7
Cell Imaging Multi-Mode Reader. Combines automated digital upright and inverted widefield microscopy with conventional multi-mode microplate reading
Both upright and inverted microscopy optics
Supports fluorescence, brightfield, and color brightfield

Information

BioTek Cytation 7 Cell Imaging Multimode Reader

BioTek Cytation 7 cell imaging multimode reader combines automated digital upright and inverted widefield microscopy with conventional multimode microplate reading. Cytation 7 includes continually variable bandpass monochromators for versatility and performance for general multimode plate reader applications.

The inverted microscopy module provides sample visualization and the upright microscopy module with reflected light imaging enables even more applications, including ELISpot and fast slide scanning. Cytation 7 is controlled by Gen5 software, which combines ease-of-use with powerful processing and analysis capabilities.

 

Features:

  • Sophisticated imaging with both upright and inverted microscopy optics offers a range of cellular and reflected light applications, yielding information in many imaging modes
  • Inverted microscopy module supports fluorescence, brightfield, and color brightfield to analyze both large objects and intracellular detail and enables a broad range of applications
  • Combination of hybrid plate reader and advanced microscopy mode provides flexible operation ready for any assay, saves time, and increases productivity in the laboratory
  • Multimode detection plus imaging saves time and data storage
  • Automation of imaging acquisition, processing, image analysis, and object count reduces manual intervention
  • Gen5 software facilitates powerful ROI identification, samples may be selected for high magnification and the process of imaging ROIs in batches can be accelerated for faster throughput
  • Take3 plate or Take3 Trio allows analysis of 16 or 48 samples in a single run, saving time compared to single-sample devices
  • Increased sensitivity with large bandwidth settings and increased specificity with small bandwidth settings enhance assay performance and flexibility for applications

 

 

 
Cytation 7 Cytation 5 Cytation 1
General
Detection modes UV-Vis absorbance UV-Vis absorbance UV-Vis absorbance
Fluorescence intensity Fluorescence intensity Fluorescence intensity
Luminescence Luminescence Luminescence
Fluorescence polarization Fluorescence polarization
Time-resolved fluorescence Time-resolved fluorescence
Alpha
Read methods Endpoint, kinetic, spectral scanning, well area scanning Endpoint, kinetic, spectral scanning, well area scanning Endpoint, kinetic, spectral scanning, well area scanning
Microplate types Monochromator: 6- to 384-well plates Monochromator: 6- to 384-well plates Monochromator: 6- to 384-well plates
Imaging: 6- to 1536-well plates Filters: 6- to 1536-well plates Filters: 6- to 1536-well plates
Imaging: 6- to 1536-well plates Imaging: 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer) Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer) Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer)
Take3 Micro-Volume Plates Take3 Micro-Volume Plates Take3 Micro-Volume Plates
Temperature control 4-Zone incubation to 45 °C with Condensation Control; 4-Zone incubation to 65 °C with Condensation Control; 4-Zone incubation to 45 °C with Condensation Control™;
+0.5 °C at 37 °C +0.2 °C at 37 °C Variation +0.2 °C at 37 °C
Cooling Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides internal cooling after incubated processes. Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides internal cooling after incubated processes. Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides internal cooling after incubated processes.
Shaking Linear, orbital, double orbital Linear, orbital, double orbital Linear, orbital, double orbital
Software Gen5™ Microplate Reader and Imager Software included Gen5™ Microplate Reader and Imager Software Gen5™ Microplate Reader and Imager Software
Gen5 Secure for 21 CFR Part 11 compliance (option) Gen5 Image+ and Image Prime software available for full image analysis Gen5 Image+ and Image Prime software available for full image analysis
Gen5 Secure available for 21 CFR Part 11
Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation BioStack and 3rd party automation compatible BioStack and 3rd party automation compatible BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible BioSpa 8 Automated Incubator compatible BioSpa 8 Automated Incubator compatible
CO2 and O2 control (option) Range: 0 – 20% (CO2); 1 – 19% (O2) Range: 0 – 20% (CO2); 1 – 19% (O2) 0 – 20% CO2 control and 1 – 19% O2 control, with optional Gas Controller
Models for both CO2 and O2 or CO2 only are available Models for both CO2 and O2 or CO2 only are available Models for both CO2 and O2 or CO2 only are available
Imaging – Inverted Microscope
Imaging mode Fluorescence, color brightfield, user-selectable brightfield/high contrast brightfield Fluorescence, brightfield, high contrast brightfield, color brightfield and phase contrast Fluorescence and high contrast brightfield (2.5x Zeiss, 4x, 10x, 20x, 40x and 60x)
Imaging method Single color, multi-color, time lapse, montage, z-stacking, z-stack montage Single color, multi-color, montage, time lapse, z-stacking Single color, multi-color, montage, time lapse, z-stacking
Image processing Z-projection, digital phase contrast, stitching Z-projection, digital phase contrast, stitching Z-projection, image stitching
Camera 16-bit Sony CMOS wide field of view monochrome camera 16-bit Sony CMOS, standard or wide FOV 16-bit gray scale, Sony
Objective capacity 6-position automated turret for user-replaceable objectives 6-position automated turret for user-replaceable objectives 2 user-replaceable objectives
Objectives available 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x
Phase objectives available 4x, 10x, 20x, 40x
Image filter cube capacity 4 user-replaceable fluorescence cubes, plus brightfield channel 4 user-replaceable fluorescence cubes plus brightfield channel 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-FRET, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, Tag BFP-FRET, GFP (Ex)-CY5 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex 377/Em 647, Oxidized roGFP2, TRITC DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647 DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647
Imaging LED cubes available 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 554 nm, 590 nm, 623 nm, 655 nm, 740 nm 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions Autofocus, user-trained autofocus, autoexposure, auto-LED intensity Autofocus, auto LED intensity, auto exposure Autofocus, auto LED intensity, auto exposure
Autofocus method Image-based autofocus Image-based autofocus Image-based autofocus
User-trained autofocus User-trained autofocus User-trained autofocus
Laser autofocus (option) Laser autofocus (option) Laser autofocus (option)
Positional controls Software Control Software control Software control
Joystick controller (option) Joystick control (option)
Image collection rate Image-based autofocus: Image-based autofocus: Image-based autofocus:
96 wells, 1 color (DAPI), 4x, 6 minutes 96 wells, 1 color (DAPI), 4x, 6 minutes 96 wells, 1 color (DAPI), 4x, 6 minutes
96 wells, 3 colors, 4x, 12 minutes 96 wells, 3 colors, 4x, 12 minutes
Laser autofocus:
Laser autofocus:
96 wells, 1 color (DAPI), 4x, <3 minutes Laser autofocus:
96 wells, 1 color (DAPI), 4x, <3 minutes
96 wells, 1 color (DAPI), 4x, <3 minutes
96 wells, 3 colors, 4x, <7 minutes, 30 seconds
96 wells, 3 colors, 4x, <7 minutes, 30 seconds
Burst Mode: 10 fps, single well, single color at <= 5Oms integration time
Burst Mode: 10 fps, single well, single color at <= 50ms integration time
Image Analysis Software option Gen5 Image+: Image analysis Gen5 Image+: Image analysis Gen5 Image+: Image analysis
Gen5 Image Prime: Advanced image analysis Gen5 Image Prime: Advanced image analysis Gen5 Image Prime: Advanced image analysis
Gen5 Secure: 21 CFR Part 11 compliant features Gen5 Secure: 21 CFR Part 11 compliant features Gen5 Secure: 21 CFR Part 11 compliant features
Imaging – Upright Microscope
Imaging modes Reflected color brightfield; transmitted color brightfield
Imaging methods Single image, montage, time lapse, Z-stacking
Image processing Z-projection, digital phase contrast, stitching
Camera 16-bit Sony CMOS wide field of view color camera
Lenses Finder scope, 2x, 4x, 8x
Positional controls Software Control
Joystick controller (option)
Image collection rate Entire 100 mm dish @ 1x: <2:40 minutes
Entire microscope slide @1x: <1:15 minutes
96-well ELISpot plate@ 1x: <5 minutes
Image Analysis Software option Gen5 Image+: Image analysis
Gen5 Image Prime: Advanced image analysis
Gen5 Secure: 21 CFR Part 11 compliant features
Fluorescence Intensity
Light source Xenon flash Xenon flash Xenon flash
Detector PMT (red-shifted PMT option) PMT for monochromator system PMT
PMT for filter system
Wavelength selection Quad monochromators (top/bottom) Quad monochromators (top/bottom) Deep blocking band pass filters / dichroic mirrors
Filters (top)
Wavelength range 250 – 700 (900 nm option) Monochromators: 250 – 700 nm (900 nm option) Filters: 200 – 700 nm (850 nm option)
Filters: 200 – 700 nm (850 nm option)
Monochromator bandwidth Variable, from 9 nm to 50 nm in 1 nm increments Variable, from 9 nm to 50 nm in 1 nm increments
Dynamic range 7 decades 7 decades 7 decades
Sensitivity Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) – top Filters: Fluorescein: 0.25 pM (0.025 fmol/well, 384-well plate)
Fluorescein 4 pM (0.4 fmol/well, 384-well plate) – bottom Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate)
Quad Monochromator:
Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) – top
Fluorescein 4 pM (0.4 fmol/well, 384-well plate) – bottom
Reading speed (kinetic) 96 wells: 11 seconds 96 wells: 11 seconds 96 wells: 11 seconds
384 wells: 22 seconds 384 wells: 22 seconds 384 wells: 22 seconds
Luminescence
Wavelength range 300 – 700 nm 300 – 700 nm 300 – 700 nm
Dynamic range >6 decades >6 decades >6 decades
Sensitivity 20 amol ATP (flash) Monos: 20 amol ATP (flash) 10 amol ATP (flash)
Filters: 10 amol ATP (flash), 100 amol (glow) 100 amol (glow)
Fluorescence Polarization
Light source Xenon flash Xenon flash
Detector PMT PMT
Wavelength selection Filters Filters
Wavelength range 280 – 700 nm (850 nm option) 400 nm – 700 nm
Sensitivity 1.2 mP standard deviation at 1 nM fluorescein 1.2 mP standard deviation at 1 nM fluorescein
Time-Resolved Fluorescence
Light source Xenon flash Xenon flash
Detector PMT PMT
Wavelength selection Quad monochromators (secondary mode) Filters
Filters (top)
Wavelength range Filters: 200 – 700 nm (850 nm option)
Sensitivity Filters: Europium 40 fM (4 amol/well, 384-well plate) Europium 40 fM (4 amol/well, 384-well plate)
Monos: Europium 1200 fM (120 amol/well, 384-well plate)
Absorbance
Light source Xenon flash Xenon flash Xenon flash
Detector Photodiode photodiode photodiode
Wavelength selection Monochromator Monochromator Monochromator
Wavelength range 230 – 999 nm, 1 nm increment 230 – 999 nm, 1 nm increment 200 – 999 nm, 1 nm increment
Monochromator bandwidth 4 nm (230 – 285 nm), 8 nm (>285 nm) 4 nm (230 – 285 nm), 8 nm (>285 nm) 2.4 nm
Dynamic range 0 – 4.0 OD 0 – 4.0 OD 0 – 4.0 OD
Resolution 0.0001 OD 0.0001 OD 0.0001 OD
Pathlength correction Yes yes yes
Monochromator wavelength accuracy +2 nm +2 nm + 2 nm
Monochromator wavelength repeatability +0.2 nm +0.2 nm + 0.2 nm
OD accuracy <1% at 3.0 OD <1% at 2.0 OD <1% at 2.0 OD
<3% at 3.0 OD <3% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD <1% from 0 to 3.0 OD <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD <0.5% at 2.0 OD <0.5% at 2.0 OD
Stray light 0.03% at 230 nm 0.03% at 230 nm 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 11 seconds 96 wells: 11 seconds 96 wells: 11 seconds
384 wells: 22 seconds 384 wells: 22 seconds 384 wells: 22 seconds
Alpha Detection
Light source 100 mW 680 nm laser
Detector PMT
Wavelength selection Filters (top)
Sensitivity 100 amol LCK peptide (384-well low volume plate)
Reagent Injectors (option)
Supported detection modes All modes All modes
Number 2 syringe pumps 2 syringe pumps 2 syringe pumps
Supported labware 6- to 384-well plates, Petri and cell culture dishes 6- to 384-well plates, Petri and cell culture dishes 6- to 384-well plates, Petri and cell culture dishes
Dead volume 1.1 mL with back flush 1.1 mL with back flush 1.1 mL with back flush
Dispense volume 5 – 1000 µL in 1 µL increments 5 – 1000 µL in 1 µL increments 5 – 1000 µL in 1 µL increments
Plate geometry 6- to 384-well microplates 6- to 384-well microplates 6- to 384-well microplates
Dispense accuracy +1 µL or 2% +1 µL or 2% +1 µL or 2%
Dispense precision <2% at 50 – 200 µL <2% at 50 – 200 µL <2% at 50 – 200 µL
Physical Characteristics
Power 150 Watts maximum consumption 250 Watts maximum consumption. 100-240 VAC, 50/60 Hz (24VDC external power supply, 150W min)
Dimensions 20.2″ D x 16.4″ W x 17.5″ H 20.2″ D x 16.4″ W x 17.5″ H 20.2″ D x 16.4″ W x 17.5″ H
(51.4 cm D x 41.6 cm W x 44.5 cm H) (51.4 cm D x 41.6 cm W x 44.5 cm H) (51.4 cm D x 41.6 cm W x 44.5 cm H)
Weight 80 lbs (36.3 kg) 80 lbs (36.3 kg) 65 lbs (29 Kg)
Regulatory
Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available. CE and TUV marked. RoHS Compliant. Models for In Vitro CE and TUV marked. Models for In Vitro Diagnostic use are available.
Diagnostic use are available.
Specifications are subject to change.

Multimode plate reader with sophisticated imaging

Cytation 7 includes both upright and inverted microscopy optics that open a wide range of cellular and reflected light applications that cannot be performed on a standard plate reader. Information on cell morphology, localization of signal, cell count, object identification and quantification can be obtained with Cytation 7 imaging modes. The monochromator plate reader optics allows running all standard plate reader assays.

Comprehensive imaging solution

The Cytation 7 inverted microscopy module supports fluorescence, brightfield, and color brightfield from 1.25x to 60x to analyze both large objects and intracellular details. The Cytation 7 upright reflected light imaging module enables a broad range of applications such as ELISpot, colony counting, material inspection, and much more.

ELISpot imaging

Cytation 7 upright imaging module can be used to automate assays such as ELISpot in which cell secretions are rendered visible through the use of a colorimetric reaction. Cytation 7 fully automates imaging acquisition, processing, image analysis and object count.  
GENERAL
Detection modes UV-Vis absorbance
Fluorescence intensity
Luminescence
Read methods Endpoint, kinetic, spectral scanning, well area scanning
Microplate types Monochromator: 6- to 384-well plates
Imaging: 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer)
Take3 Micro-Volume Plates
Temperature control 4-Zone incubation to 45 °C with Condensation Control; +0.5 °C at 37 °C
Cooling Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides internal cooling after incubated processes.
Shaking Linear, orbital, double orbital
Software Gen5™ Microplate Reader and Imager Software included
Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
CO2 and O2 control (option) Range: 0 – 20% (CO2); 1 – 19% (O2)
Models for both CO2 and O2 or CO2 only are available
IMAGING – INVERTED MICROSCOPE
Imaging mode Fluorescence, color brightfield, user-selectable brightfield/high contrast brightfield
Imaging method Single color, multi-color, time lapse, montage, z-stacking, z-stack montage
Image processing Z-projection, digital phase contrast, stitching
Camera 16-bit Sony CMOS wide field of view monochrome camera
Objective capacity 6-position automated turret for user-replaceable objectives
Objectives available 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x
Image filter cube capacity 4 user-replaceable fluorescence cubes, plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-FRET, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, Tag BFP-FRET, GFP (Ex)-CY5 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex 377/Em 647, Oxidized roGFP2, TRITC
Imaging LED cubes available 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 554 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions Autofocus, user-trained autofocus, autoexposure, auto-LED intensity
Autofocus method Image-based autofocus
User-trained autofocus
Laser autofocus (option)
Positional controls Software Control
Joystick controller (option)
Image collection rate Image-based autofocus:
96 wells, 1 color (DAPI), 4x, 6 minutes
Laser autofocus:
96 wells, 1 color (DAPI), 4x, <3 minutes
Image Analysis Software option Gen5 Image+: Image analysis
Gen5 Image Prime: Advanced image analysis
Gen5 Secure: 21 CFR Part 11 compliant features
IMAGING – UPRIGHT MICROSCOPE
Imaging modes Reflected color brightfield; transmitted color brightfield
Imaging methods Single image, montage, time lapse, Z-stacking
Image processing Z-projection, digital phase contrast, stitching
Camera 16-bit Sony CMOS wide field of view color camera
Lenses Finder scope, 2x, 4x, 8x
Positional controls Software Control
Joystick controller (option)
Image collection rate Entire 100 mm dish @ 1x: <2:40 minutes
Entire microscope slide @1x: <1:15 minutes
96-well ELISpot plate@ 1x: <5 minutes
Image Analysis Software option Gen5 Image+: Image analysis
Gen5 Image Prime: Advanced image analysis
Gen5 Secure: 21 CFR Part 11 compliant features
FLUORESCENCE INTENSITY
Light source Xenon flash
Detector PMT (red-shifted PMT option)
Wavelength selection Quad monochromators (top/bottom)
Wavelength range 250 – 700 (900 nm option)
Monochromator bandwidth Variable, from 9 nm to 50 nm in 1 nm increments
Dynamic range 7 decades
Sensitivity Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) – top
Fluorescein 4 pM (0.4 fmol/well, 384-well plate) – bottom
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
LUMINESCENCE
Wavelength range 300 – 700 nm
Dynamic range >6 decades
Sensitivity 20 amol ATP (flash)
ABSORBANCE
Light source Xenon flash
Detector Photodiode
Wavelength selection Monochromator
Wavelength range 230 – 999 nm, 1 nm increment
Monochromator bandwidth 4 nm (230 – 285 nm), 8 nm (>285 nm)
Dynamic range 0 – 4.0 OD
Resolution 0.0001 OD
Pathlength correction Yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
REAGENT INJECTORS (OPTION)
Number 2 syringe pumps
Supported labware 6- to 384-well plates, Petri and cell culture dishes
Dead volume 1.1 mL with back flush
Dispense volume 5 – 1000 µL in 1 µL increments
Plate geometry 6- to 384-well microplates
Dispense accuracy +1 µL or 2%
Dispense precision <2% at 50 – 200 µL
PHYSICAL CHARACTERISTICS
Power 150 Watts maximum consumption
Dimensions 20.2″ D x 16.4″ W x 17.5″ H
(51.4 cm D x 41.6 cm W x 44.5 cm H)
Weight 80 lbs (36.3 kg)
REGULATORY
Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.
The Peltier Cooling Module for BioTek’s Cytation™ 5 and Cytation 1 Cell Imaging Multi-Mode Readers helps maintain temperature stability inside the reading chamber, regardless of changes in ambient (room) temperature and heat produced by electro-mechanical operations within the reader. The compact Peltier Cooling Module attaches easily to Cytation’s rear case. (See image)
Instrument without Peltier Cooling Module: The instrument’s interior temperature (red) follows ambient room temperature (green). It is also significantly warmer than ambient temperature because of heat produced by the instrument. This can result in assay data variation through the day, or drifts for kinetic assays run at ambient temperature
Instrument with Peltier Cooling Module: The instrument’s interior temperature (blue) is stabilized which results in more consistent assay data, and higher quality room temperature kinetic data
Benefits of the Peltier Cooling Module for Cytation: • Helps maintain the instrument’s internal ambient temperature stability, with less than 1 °C rise over external ambient, enabling more consistent results • Quickly reduces internal temperature following incubated assays up to 3x faster, to allow both incubated and ambient assays to be run more efficiently in multi-user environments. Peltier Cooling Module is available for: • Cytation Multi-Mode Reader • Cytation 5 Cell Imaging Multi-Mode Reader • Cytation 1 Cell Imaging Multi-Mode Reader

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