BioTek Cytation 1

Cell Imaging Multi-Mode Reader. Combine fluorescence and high contrast brightfield imaging with conventional multi-mode detection

Information

Cytation1 Cell Imaging Multi-Mode Reader combines fluorescence and high contrast brightfield imaging with conventional multi-mode detection without the expense and complexity commonly associated with other digital microscopy systems. This unique, patented design provides both quantitative phenotypic cellular information with well-based quantitative data. Cytation 1’s multi-mode detection module includes high sensitivity filter-based fluorescence and a monochromator system for UV-Vis absorbance. Temperature control and shaking are standard; CO2/O2 control and reagent injectors are available. BioTek’s powerful Gen5 software makes image capture and plate reading easy.

Affordable, patented, quantitative digital microscopy and optional multi-mode microplate detection
Augmented Microscopy™ using Gen5 software for seamless image capture to quantitative publication ready data with no extensive training required
Fluorescence and high contrast brightfield imaging from 1.25x to 60x to capture large regions of interest to 60x or intracellular details
Affordable automation: automated XY stage, focus, exposure, image capture and LED intensity
Cell friendly design – 4-Zone incubation to 45°C with Condensation Control, CO2/O2 control, angled injectors and lidded plate handling
High performance filter-based fluorescence detection with convenience of monochromator based UV-Vis absorbance

Have you read the testimonial of Joëlle J.E. Janssen about how she used the Cytation 1 during her PhD? Read it here: The Cytation 1 enabled me to obtain high-quality brightfield images of white blood cells

Imaging Plus Multi-Mode Configurations

	CYT1FAV	CYT1FA	CYT1V
Fluorescence and high contrast brightfield imaging	•		•
Monochromator absorbance	•	•
Filter/dichroic fluorescence	•	•
Luminescence / filtered luminescence	•	•

GENERAL
Detection modes	UV-Vis absorbance
	Fluorescence intensity
	Luminescence
	Fluorescence polarization
	Time-resolved fluorescence
Read methods	Endpoint, kinetic, spectral scanning, well area scanning
Microplate types	Monochromator: 6- to 384-well plates
	Filters: 6- to 1536-well plates
	Imaging: 6- to 1536-well plates
Other labware supported	Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer)
Other labware supported	Take3 Micro-Volume Plates
Temperature control	4-Zone incubation to 45 °C with Condensation Control™; Variation +0.2 °C at 37 °C
Shaking	Linear, orbital, double orbital
Software	Gen5™ Microplate Reader and Imager Software
	Gen5 Image+ and Image Prime software available for full image analysis
	Gen5 Secure available for 21 CFR Part 11
Automation	BioStack and 3rd party automation compatible
Automation	BioSpa 8 Automated Incubator compatible
CO2 and O2 control (option)	0 – 20% CO2 control and 1 – 19% O2 control, with optional Gas Controller
CO2 and O2 control (option)	Models for both CO2 and O2 or CO2 only are available
IMAGING SYSTEM
Imaging mode	Fluorescence
Imaging mode	High Contrast Brightfield (2.5x Zeiss, 4x, 10x, 20x, 40x and 60x)
Imaging method	Single color, multi-color, montage, time lapse, z-stacking
Image processing	Z-projection, image stitching
Camera	16-bit gray scale, Sony CCD
Objective capacity	2 user-replaceable objectives
Objectives available	1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x
Image filter cube capacity	4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available	DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647
Imaging LED cubes available	365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions	Autofocus, auto LED intensity, auto exposure
Autofocus method	Image-based autofocus
	User-trained autofocus
	Laser autofocus (option)
Positional controls	Software control
Image collection rate	Image-based autofocus:
	96 wells, 1 color (DAPI), 4x, 6 minutes
	96 wells, 3 colors, 4x, 12 minutes

	Laser autofocus:
	96 wells, 1 color (DAPI), 4x, <3 minutes
	96 wells, 3 colors, 4x, <7 minutes, 30 seconds Burst Mode: 10 fps, single well, single color at <= 50ms integration time
Image Analysis Software option	Gen5 Image+: Image analysis
	Gen5 Image Prime: Advanced image analysis
	Gen5 Secure Image+: Advanced image analysis, 21 CFR Part 11 features
FLUORESCENCE INTENSITY
Light source	Xenon flash
Detector	PMT
Wavelength selection	Deep blocking band pass filters / dichroic mirrors
Wavelength range	Filters: 200 – 700 nm (850 nm option)
Dynamic range	7 decades
Sensitivity	Fluorescein: 0.25 pM (0.025 fmol/well, 384-well plate)
Reading speed (kinetic)	96 wells: 11 seconds
Reading speed (kinetic)	384 wells: 22 seconds
LUMINESCENCE
Wavelength range	300 – 700 nm
Dynamic range	>6 decades
Sensitivity	10 amol ATP (flash)
Sensitivity	100 amol (glow)
FLUORESCENCE POLARIZATION
Light source	Xenon flash
Detector	PMT
Wavelength selection	Filters
Wavelength range	400 nm – 700 nm
Sensitivity	1.2 mP standard deviation at 1 nM fluorescein
TIME-RESOLVED FLUORESCENCE
Light source	Xenon flash
Detector	PMT
Wavelength selection	Filters
Sensitivity	Europium 40 fM (4 amol/well, 384-well plate)
ABSORBANCE
Light source	Xenon flash
Detector	photodiode
Wavelength selection	Monochromator
Wavelength range	200 – 999 nm, 1 nm increment
Monochromator bandwidth	2.4 nm
Dynamic range	0 – 4.0 OD
Resolution	0.0001 OD
Pathlength correction	yes
Monochromator wavelength accuracy	+ 2 nm
Monochromator wavelength repeatability	+ 0.2 nm
OD accuracy	<1% at 2.0 OD
OD accuracy	<3% at 3.0 OD
OD linearity	<1% from 0 to 3.0 OD
OD repeatability	<0.5% at 2.0 OD
Stray light	0.03% at 230 nm
Reading speed (kinetic)	96 wells: 11 seconds
Reading speed (kinetic)	384 wells: 22 seconds
REAGENT INJECTORS (OPTION)
Supported detection modes	All modes
Number	2 syringe pumps
Supported labware	6- to 384-well plates, Petri and cell culture dishes
Dead volume	1.1 mL with back flush
Dispense volume	5 – 1000 µL in 1 µL increments
Plate geometry	6- to 384-well microplates
Dispense accuracy	+1 µL or 2%
Dispense precision	<2% at 50 – 200 µL
PHYSICAL CHARACTERISTICS
Power	100-240 VAC, 50/60 Hz (24VDC external power supply, 150W min)
Dimensions	20″ D x 16.5″ W x 17.5″ H
Dimensions	(50.8 cm x 41.91 cm x 44.5 cm)
Weight	65 lbs (29 Kg)
REGULATORY
Regulatory	CE and TUV marked. Models for In Vitro Diagnostic use are available.

The Peltier Cooling Module for Agilent’s Cytation™ 5 and Cytation 1 Cell Imaging Multi-Mode Readers helps maintain temperature stability inside the reading chamber, regardless of changes in ambient (room) temperature and heat produced by electro-mechanical operations within the reader. The compact Peltier Cooling Module attaches easily to Cytation’s rear case. (See image)

Instrument without Peltier Cooling Module: The instrument’s interior temperature (red) follows ambient room temperature (green). It is also significantly warmer than ambient temperature because of heat produced by the instrument. This can result in assay data variation through the day, or drifts for kinetic assays run at ambient temperature

Instrument with Peltier Cooling Module: The instrument’s interior temperature (blue) is stabilized which results in more consistent assay data, and higher quality room temperature kinetic data

Benefits of the Peltier Cooling Module for Cytation: • Helps maintain the instrument’s internal ambient temperature stability, with less than 1 °C rise over external ambient, enabling more consistent results • Quickly reduces internal temperature following incubated assays up to 3x faster, to allow both incubated and ambient assays to be run more efficiently in multi-user environments. Peltier Cooling Module is available for: • Cytation Multi-Mode Reader • Cytation 5 Cell Imaging Multi-Mode Reader • Cytation 1 Cell Imaging Multi-Mode Reader

Collect both phenotypic data and quantitative data from one instrument. Image and data analysis are provided with Gen5 software for all applications Patented Hybrid optics: sensitivity of filters, flexibility of monochromator Cytation 5’s hybrid optical design offers filter-based fluorescence optics for high transmission and high sensitivity, while the available monochromator optics offers convenience and assay flexibility. Multiple fluorescence measurement modes include fluorescence intensity, time-resolved fluorescence and fluorescence polarization Augmented Microscopy™ using Gen5 software: image capture to quantitative publication ready data. Gen5 software takes you automatically through the steps typically performed in microscopy: capture > process > analyze > publish. The interface is logical and user-friendly, so your imaging workflows can be up and running without extensive training. Fluorescence and high contrast brightfield imaging The powerful imaging module in Cytation 1 offers four color channels plus brightfield, from 1.25x for imaging larger samples to 60x for intracellular details. More than 15 filter/LED cubes are available, supporting a broad range of imaging applications. Affordable automation Cytation 1 has an automated XY stage, autofocus, auto exposure and auto LED intensity for efficient capture of high quality images. The automated features in Cytation 1 and Gen5 make it easy for you to find the region of interest, then quickly find the best settings to capture the images. Gen5 software is simple to use, without extensive training. Cell friendly design Live cell assays require an optimal environment. Cytation 1 has temperature control and shaking, plus CO2 / O2 control, to facilitate longer-term kinetic studies. High performance and convenience To optimize multi-mode detection, Cytation 1 has high performance filters for fluorescence and monochromator-based UV-Vis absorbance for convenient wavelength selection in 1 nm increments.

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Imaging Plus Multi-Mode Configurations

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