BioTek Lionheart LX

Agilent

BioTek Lionheart LX

Short product description

Automated digital endpoint microscope

Information

The Lionheart LX Automated Microscope is designed with affordability in mind, offering an alternative to expensive, custom-built automated microscopes that are complex to learn and use. The compact, ocular-free hardware design eases ergonomic discomfort. Along with Gen5™ software, Lionheart LX enables Augmented Microscopy™, which fully automates image capture, processing and analysis. Load samples, start a run and come back to publication-ready images and quantitative data. Lionheart LX can help increase your research output while reducing processing time and costs.

Features:

Affordable automated digital microscopy
High contrast brightfield, color brightfield and fluorescence imaging

Augmented Microscopy: Easy imaging workflow automation

Quick analyze: instant cell count and confluence measurement
Integrated, compact design offers quick installation and setup
Small benchtop footprint

Specifications

GENERAL
Microplate types	6- to 1536-well plates
Other labware supported	Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides
Other labware supported	Support for labware up to 1.5″ tall
Software	Gen5 Microplate Reader and Imager Software included
Software	Gen5 Image and Image+ Software available for advanced image analysis (option)
X/Y Stage resolution	Lead screw driven stage with 0.1 micron resolution
IMAGING SYSTEM
Imaging mode	Fluorescence, high contrast brightfield and color brightfield
Imaging method	Single color, multi-color, montage, time lapse, Z-stack, z-stack montage, burst mode
Light source	High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm)
Camera	16-bit CMOS camera. 2.3 MP Sony IMX249 1/1.2″ sensor with 5.86 micron pixel size
Camera binning	Optional 2×2 binning for focus and/or image capture
Camera exposure range	5 milliseconds to 4 seconds
Image outputs available	Raw Images: 16-bit TIFF
	Saved Images: TIFF, JPG, BMP, PNG, EMF, GIF, color TIFF
	Movies: MP4, WMV
Objective capacity	6 onboard, user-replaceable objectives
Objectives available	Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA.60; 60x, NA .70
	Oil: 60x, NA:1.42; 60x, NA 1.25; 100x, NA 1.4; 100x, NA 1.3
	High NA: 20x, NA 0.75; 40x, NA 0.95
Image filter cube capacity	4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available	DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647
Automated functions	Autofocus, user-trained autofocus, auto exposure, auto-LED intensity
Autofocus method	Image-based autofocus
Autofocus method	Laser autofocus option
Image collection rate	Single well fastest frame rate capture:
	*Full Resolution: up to 10 frames per second for single color images
	*2×2 Binning: up to 20 frames per second for single color images
Microscope stage control	Gen5 Software Control
Microscope stage control	Optional joystick controller
PHYSICAL CHARACTERISTICS
Power	250 Watts max.
Dimensions	18.3″ D, 17.9″ W, 14.1″ H (46.5 cm x 45.5 cm x 35.8 cm)
Weight	51 lbs (23.1 kg)
REGULATORY
Regulatory	CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available.

Configurations

LLX
Lionheart LX Automated Microscope. Fluorescence, high contrast brightfield and color brightfield imaging. Includes standard Gen5 software and 4-position slide holder. The imaging controller, objectives, filter/LED cubes, accessories and software upgrades are sold separately.
Software

Gen5 Microplate Reader and Imager Software controls Lionheart LX for automated image capture, processing, analysis and development of publication-ready images and data. This imaging and microscopy automation represents BioTek’s Augmented Microscopy™ – the simplified, automated process to accomplish multiple microscopy steps in a single platform. Gen5 is available in several editions to meet the image analysis requirements across a range of applications.

Gen5 Image+ offers complete imaging control for Lionheart LX Automated Microscope, and provides image processing and analysis including z-projection, image stitching, hitpicking and digital phase contrast.

Gen5 Image Prime controls Lionheart LX Automated Microscope includes more expansive image processing and analysis: Z-projection, image stitching, hitpicking and digital phase contrast, plus advanced features including secondary mask, dynamic thresholding and subpopulation analysis.

Gen5 Secure editions add features to help ensure compliance to 21 CFR Part 11 requirements.

Gen5 add-on modules provide increased functionality for specific applications or analyses, such as Spot Counting.
Features

Affordable automated digital microscopy.
Lionheart LX has an automated 6-objective turret and can contain up to 4 LED/filter cubes for multi-channel image capture. The precise automated stage and autofocus, auto exposure and other Gen5 software features enable simple, automated image capture and analysis.

High contrast brightfield, color brightfield and fluorescence imaging
With brightfield, color brightfield and fluorescence imaging in four channels – and more than twenty available filter/LED cubes, Lionheart LX offers fantastic versatility that isn’t limited to image capture. Imaging processes from z-stacking to z-projection, montage collection to stitching; plus analysis from label-free cell counting to mitochrondrial membrane potential are all accomplished with Lionheart LX and Gen5 software.

Augmented Microscopy: Easy imaging workflow automation
BioTek’s Augmented Microscopy automates image capture, processing and analysis for publication-ready images and data. Automated image capture starts with image-based and laser autofocus, plus auto LED and exposure. Automated image pre-processing optimizes images for downstream analysis, from cell counting to characterization of subcellular details

Quick analyze: instant cell count and confluence measurement
Get cell counts and confluence calculations directly on the live camera feed. Gen5 software controls Lionheart LX to quickly find the region of interest and display counts onscreen without needing to capture the image. The quick analyze function works for samples in a variety of labware.

Integrated, compact design offers quick installation and setup
Lionheart LX integrates all microscopy hardware into a very compact footprint, saving valuable bench space. The easily accessible objectives and LED/filter cubes make setup and operation fast and simple, with a minimal learning curve.

Brochures

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