BioTek Synergy LX - Multimode reader
Entry-level Multi-Mode Microplate Reader
Easy-to-use microplate reader with uncompromised performance for UV-Vis absorbance, fluorescence, and luminescence assays
High performance

Information

The BioTek Synergy LX multimode reader automates many common microplate assays. The high-quality optics design ensures excellent data in UV-Vis absorbance, fluorescence, and luminescence detection modes. This affordable system is ideal for many common assays including nucleic acid and protein quantification, ELISA, BCA, Bradford, and cell viability.

Gen6 data analysis software provides complete reader control and data analysis, and includes many pre-defined experiments, ready to customize for your assays. Synergy LX is available with a large touch screen user interface and onboard software for endpoint measurements and analysis.

 

Key features:

  • An affordable multimode microplate reader, Synergy LX is the solution for labs looking for an easy-to-use microplate reader that offers uncompromised performance for UV-Vis absorbance, fluorescence, and luminescence assays
  • Synergy LX delivers excellent performance for a broad range of applications, including nucleic acid and protein quantification, ELISA, gene expression, and cell viability assays
  • Micro-volume quantification with Take3 plates enables direct DNA, RNA, and protein quantification of precious samples, using 2 µL sample volumes. Get rapid results for 16 or to 48 samples at a time, without additional instrumentation
  • Continuous UV-Vis wavelength selection from 200 to 999 nm with a broad, filter-free wavelength range for absorbance measurements. Performance in colorimetric and UV applications including nucleic acid and protein quantification, and turbidity measurements
  • High performance, high blocking filters for fluorescence and luminescence enable uncompromised performance. High-quality filters with dichroic mirrors efficiently block background noise and provide excellent sensitivity in fluorescent measurements
  • The available touch screen configuration offers easy operation and immediate data display for quick set up and operation. The data is displayed immediately after the run and a color gradient helps to easily view the data range
  • Gen6 data analysis software delivers walk-up usability for assay setup and data analysis. Convenient prebuilt experiment templates for all detection modes provide a quick start
Absorbance Fluorescence Luminescence Touchscreen
SLXA
SLXF
SLXFA
SLXATS
SLXFTS
SLXFATS
GENERAL
Detection modes UV-Vis absorbance
Fluorescence intensity
​Luminescence
Read methods Endpoint (onboard software)
Endpoint, kinetic, area scanning, absorbance spectral scanning (under Gen6 control)
Microplate types UV-Vis absorbance: 6- to 384-well plates (onboard software)
Fluorescence intensity and luminescence: 96- and 384-well (onboard software)
All modes: 6- to 384-well microplates (under Gen6 control)
Other labware supported Take3 Micro-Volume Plates (onboard software)
Take3 and Take3 Trio Micro-Volume Plates (under Gen5 control)
Shaking Linear, orbital, double-orbital
Software Endpoint protocols (onboard)
Full data analysis and reporting (under Gen6 control)
ABSORBANCE
Light source Xenon flash
Detector photodiode
Wavelength selection monochromator
Wavelength range 200 nm to 999 nm, in 1 nm increments
Monochromator bandwidth < 5 nm
Dynamic range 0 to 4.000 OD
Resolution 0.001 OD (onboard software)
0.0001 OD (under Gen6 control)
Pathlength correction yes
Monochromator wavelength accuracy + 2 nm
Monochromator wavelength repeatability + 0.2 nm (standard deviation)
OD accuracy <1% at 2.0 OD
<3% at 2.5 OD
OD linearity <1% from 0 to 2.5 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 12 seconds
384 wells: 23 seconds
FLUORESCENCE INTENSITY
Light source Halogen
Detector PMT
Wavelength selection Filters
Wavelength range 320-700 nm (low noise PMT)
320-850 nm (red-shifted PMT)
Dynamic range >6 decades
Sensitivity Fluorescein 2 pM
Reading speed (kinetic) 96 wells: 24 secs
384 wells: 76 secs
LUMINESCENCE
Dynamic range >6 decades
Sensitivity 10 amol ATP
PHYSICAL CHARACTERISTICS
Connectivity One USB 2.0 port for computer control
Two USB 2.0 ports for printer connection and USB flash drive (touchscreen configurations only)
Power External 24VDC power supply compatible with 100-240 volts AC @50-60Hz.
60W maximum consumption.
Dimensions 15” H x 15” W x 15” D (with touchscreen)
(38.1 cm H x 38.1 cm W x 38.1 cm D)
12” H x 15” W x 15” D
(30.5 cm H x 38.1 cm W x 38.1 cm D)
Weight < 27 lbs (12.3 kg)
REGULATORY
Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.
Micro-volume quantification is fast and easy with the Take3™ and Take3 Trio Micro-Volume plates, used in your BioTek microplate reader. Measure multiple 2 µL samples at a time, without diluting and without needing specialized equipment. Pre-programmed nucleic acid and protein protocols in Gen6 make quantification fast and easy.
  • Low volume 2 µL nucleic acid and protein quantification
  • Pre-programmed protocols in Gen 6 Software
  • Absorbance and fluorescence detection
  • Easy, wipe-off maintenance
  • Read 2 µL microspots, BioCells or standard cuvettes
BioTek’s robotic instruments can help automate workflows in your laboratory. Many life science workflows benefit from expanded automation, for increased throughput or process efficiency. BioTek offers unique automation solutions that integrate with our microplate washers, dispensers, readers and imagers. BioStack is a compact stacker that offers fast plate exchange options for 50 plates or more, and patented plate de-lidding and re-lidding with BioStack 4 . BioSpa 8 Automated Incubator links BioTek readers or imagers together with washers and dispensers for full workflow automation of up to 8 microplates. Temperature, CO2/O2 and humidity levels are controlled and monitored through the BioSpa software to maintain an ideal environment for cell cultures during all experimental stages.
Affordable multi-mode reader At half the price of similar instruments, Synergy LX is the solution for labs looking for an easy to use microplate reader that offers uncompromised performance for UV-Vis absorbance, fluorescence and luminescence assays. Supports common endpoint assays Synergy LX, can run the most common assays including: • A260, A260/A280 • Protein quantification • Fluorescence, luminescence • ELISA • Gene expression • Cell viability Micro-volume quantification with Take3 plates Micro-volume analysis with BioTek’s Take3 plate and Synergy LX is fast and easy, too. Pre-programmed nucleic acid and protein protocols in Synergy LX display immediate results for up to sixteen, 2 µL samples. Output results to a USB flash drive for use in downstream workflows. Continuous UV-Vis wavelength selection: 200 nm to 999 nm The broad, filter-free wavelength range for absorbance measurements enables many common colorimetric and UV applications, from nucleic acid and protein quantitification to turbidity measurements and more. High performance, high blocking filters for fluorescence and luminescence There’s no compromise on performance in Synergy LX. The high quality filters, coupled with dichroic mirrors in the Synergy LX filter cubes efficiently block background to ensure the best sensitivity for fluorescence measurements. Touchscreen: Easy operation, immediate data display It takes only a few touches to select and run your assay. The data is displayed immediately after the run – a color gradient helps to quickly visualize the data range. Output to USB flash drive, printer or Gen6 Software With one touch, export data to a USB flash drive for analysis in BioTek’s Gen6 software or a spreadsheet application.

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