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BioTek Synergy Neo2
Information
Synergy™ Neo2 Multi-Mode Microplate Reader delivers the speed and exceptional performance required for demanding applications. Its patented Hybrid Technology™ , with filter- and monochromator- based optics including multiple PMTs, ensures ultrafast, high performance in all detection modes without compromise.
- Agilent BioTek’s fastest, highest performing multi-mode microplate reader
- Scientific quad monochromators with continuously variable bandwidth from 3 nm to 50 nm
- Live cell assay optimized: CO2/O2 control, incubation to 65 °C and direct bottom illumination
- Multiple PMTs including dual top PMTs for ultra-fast ratiometric assays
- Gen6 Data Analysis Software; Gen6 Secure for 21 CFR Part 11 compliance
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Specifications
GENERAL Detection modes UV-Vis absorbance Fluorescence intensity Luminescence Fluorescence polarization Time-resolved fluorescence Alpha Read methods Endpoint, kinetic, spectral scanning, well area scanning Microplate types 6- to 1536-well plates Other labware supported Petri and cell culture dishes Take3 Micro-Volume Plates Temperature control 4-Zone incubation to 65 °C with Condensation Control™ +0.2 °C at 37 °C Shaking Linear, orbital, double orbital Software Gen6™ Microplate Reader and Imager Software Gen5 Secure for 21 CFR Part 11 compliance (option) Automation BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible CO2 and O2 control (option) Range: 0 – 20% (CO2); 1 – 19% (O2) Control Resolution: + 0.1% (CO2 and O2) Stability: +0.2% at 5% CO2; +0.2%at 1% O2 Models for CO2 /O2 or CO2 only are available Barcode reader 1D and 2D camera-based scanner ABSORBANCE Light source Xenon flash Detector photodiode Wavelength selection monochromator Wavelength range 230 – 999 nm, 1 nm increments Monochromator bandwidth 2 nm (230-285 nm), 4 nm (>285 nm) Dynamic range 0 – 4.0 OD Resolution 0.0001 OD Pathlength correction yes Monochromator wavelength accuracy +2 nm Monochromator wavelength repeatability +0.2 nm OD accuracy <1% at 2.0 OD <3% at 3.0 OD OD linearity <1% from 0 to 3.0 OD OD repeatability <0.5% at 2.0 OD Stray light 0.03% at 230 nm Reading speed (kinetic) 96 well: 6 seconds 384 well: 11 seconds 1536 well: 25 seconds FLUORESCENCE INTENSITY Light source Xenon flash Detector Dual top PMTs Single top PMT (option) Low noise PMT (bottom filter system) Red shifted PMT (top/bottom monochromator system) Wavelength selection Quad monochromators (top/bottom) Filters (top/bottom) Wavelength range Monochromators: 250 – 850 nm Filters (dual PMT): 200 – 850 nm Monochromator bandwidth Variable, from 3 to 50 nm in 1 nm increments Dynamic range 7 decades Sensitivity Filters: Fluorescein 0.2 pM (4 amol/well, 20 µL, 384-well low volume plate) – top Fluorescein 1 pM (10 amol/well, 10 µL, 1536-well plate) – top Fluorescein 1 pM (0.1 fmol/well, 100 µL, 384-well plate) – bottom Quad monochromator: Fluorescein 2 pM (40 amol/well, 20 µL, 384-well low volume plate) – top Fluorescein 2.5 pM (0.25 fmol/well, 100 µL, 384-well plate) – bottom Reading speed (kinetic) 96 well: 6 seconds 384 well: 11 seconds 1536 well: 25 seconds LUMINESCENCE Wavelength range 300 – 700 nm Dynamic range > 6 decades Sensitivity 5 amol ATP typical (384-well low volume plate) FLUORESCENCE POLARIZATION Light source Xenon flash Detector Dual PMT or single PMT (option) Wavelength selection Filters Wavelength range 280 – 850 nm Sensitivity 1 mP standard deviation at 1 nM fluorescein (384-well low volume plate) 1.5 mP standard deviation at 1 nM fluorescein (1536-well plate) TIME-RESOLVED FLUORESCENCE Light source Xenon flash Detector Dual PMT or single PMT (option) Wavelength selection Quad monochromators (top/bottom) Filters (top/bottom) Wavelength range Monos: 250 – 850 nm Filters (dual PMT): 200 – 850 nm Sensitivity Europium 40 fM (384-well low volume plate) Europium 70 fM (1536-well plate) ALPHA DETECTION Light source 100 mW 680 nm laser Detector PMT Wavelength selection Filters (top) Sensitivity 100 amol LCK peptide (384-well low volume plate) Read speed 96 well: 30 seconds 384 well: 1 minute 50 seconds 1536 well: 7 minutes 20 seconds REAGENT DISPENSERS Supported detection modes All modes Number 2 syringe pumps Supported labware 6- to 384-well plates, Petri dishes Dead volume 1.1 mL with back flush Dispense volume 5 – 1000 µL in 1 µL increments Dispense accuracy + 1 µL or 2% Dispense precision < 2% at 50-200 µL PHYSICAL CHARACTERISTICS Power 250 Watts max. Dimensions 15.4″ W x 20.7″ D x 16.1″ H (39 x 52.5 x 41 cm) Weight 78 lbs (35 kg) REGULATORY Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available. -
Software
Gen6 software controls all BioTek automated microplate readers, configuring system and data collection parameters, analysis and a variety of reporting and exporting features. One-click export to Excel® makes it fast and easy to export displayed data.
Gen5 Secure software is available for GxP compliant laboratories with features that support 21 CFR Part 11 rules. -
Micro Volume Plates
Micro-volume quantification is fast and easy with the Take3™ and Take3 Trio Micro-Volume plates, used in your BioTek microplate reader. Measure multiple 2 µL samples at a time, without diluting and without needing specialized equipment. Pre-programmed nucleic acid and protein protocols in Gen6 make quantification fast and easy.
- Low volume 2 µL nucleic acid and protein quantification
- Pre-programmed protocols in Gen6 Software
- Absorbance and fluorescence detection
- Easy, wipe-off maintenance
- Read 2 µL microspots, BioCells or standard cuvettes
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Robotics
BioTek’s robotic instruments can help automate workflows in your laboratory. Many life science workflows benefit from expanded automation, for increased throughput or process efficiency. BioTek offers unique automation solutions that integrate with our microplate washers, dispensers, readers and imagers. BioStack is a compact stacker that offers fast plate exchange options for 50 plates or more, and patented plate de-lidding and re-lidding with BioStack 4 . BioSpa 8 Automated Incubator links BioTek readers or imagers together with washers and dispensers for full workflow automation of up to 8 microplates. Temperature, CO2/O2 and humidity levels are controlled and monitored through the BioSpa software to maintain an ideal environment for cell cultures during all experimental stages.
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Features
BioTek’s fastest, highest performing multi-mode microplate reader.
With kinetic reading speeds as fast as 6 seconds, Synergy Neo2 is a workhorse in the HTS lab. Integrating the compact BioStack enables rapid, automated multiple plate processing for increased efficiency and throughput.Scientific quad monochromators with continuously variable bandwidth from 3 nm to 50 nm
Synergy Neo2 has quadruple scientific monochromators, with the fluorescence module offering variable bandwidths from 3 nm to 50 nm, selectable in 1 nm increments. Variable bandwidths allow you to optimize detection conditions for fluorophores that have unique excitation or emission parameters, for example, a narrow Stokes shift, to provide better performance than conventional monochromators.Live cell assay optimized: CO2/O2 control, incubation to 65 °C and direct bottom illumination
Temperature control to 65 °C, plus linear and orbital shaking and an available gas controller module optimize the environment in Synergy Neo2 for live cell assays. The dedicted filter-based bottom optical path directly illuminates the plate bottom, increasing the sensitivity of live cell measurements, while a dual reagent injector provides quick inject/image capability for observing fast reactions.Multiple PMTs including dual top PMTs for ultra-fast ratiometric assays
Up to 4 PMTs are available in the highly configurable, modular Synergy Neo2, for ultra-fast measurements in all detection modes. Two top PMTs meet the requirements for ultrafast ratiometric assays.Gen6 Data Analysis Software; Gen5 Secure for 21 CFR Part 11 compliance
Gen6 Microplate Reader and Imager Software doesn’t compromise on usability or data analysis performance – protocol creation is intuitive and flexible, and the data analysis capability rivals standalone packages. For the GxP lab, Gen5 Secure software offers features to help comply with 21 CFR Part 11, including electronic signature, data security and multiple level permissions.Winner of the SelectScience® Reviewers’ Choice Award for Drug Discovery & Development Product of the Year 2017
Synergy Neo2 consistently rates high among users – noted by the SelectScience Reviewers’ Choice Award for Drug Discovery & Development Product of the Year 2017 and Scientists’ Choice Awards® Best New Life Sciences Product of 2015.
Brochures
Application notes
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