Synergy™ Neo2 Multi-Mode Microplate Reader delivers the speed and exceptional performance required for demanding applications. Its patented Hybrid Technology™ , with filter- and monochromator- based optics including multiple PMTs, ensures ultrafast, high performance in all detection modes without compromise.
GENERAL | |
Detection modes | UV-Vis absorbance |
Fluorescence intensity | |
Luminescence | |
Fluorescence polarization | |
Time-resolved fluorescence | |
Alpha | |
Read methods | Endpoint, kinetic, spectral scanning, well area scanning |
Microplate types | 6- to 1536-well plates |
Other labware supported | Petri and cell culture dishes |
Take3 Micro-Volume Plates | |
Temperature control | 4-Zone incubation to 65 °C with Condensation Control™ |
+0.2 °C at 37 °C | |
Shaking | Linear, orbital, double orbital |
Software | Gen6™ Microplate Reader and Imager Software |
Gen5 Secure for 21 CFR Part 11 compliance (option) | |
Automation | BioStack and 3rd party automation compatible |
BioSpa 8 Automated Incubator compatible | |
CO2 and O2 control (option) | Range: 0 – 20% (CO2); 1 – 19% (O2) |
Control Resolution: + 0.1% (CO2 and O2) | |
Stability: +0.2% at 5% CO2; +0.2%at 1% O2 | |
Models for CO2 /O2 or CO2 only are available | |
Barcode reader | 1D and 2D camera-based scanner |
ABSORBANCE | |
Light source | Xenon flash |
Detector | photodiode |
Wavelength selection | monochromator |
Wavelength range | 230 – 999 nm, 1 nm increments |
Monochromator bandwidth | 2 nm (230-285 nm), 4 nm (>285 nm) |
Dynamic range | 0 – 4.0 OD |
Resolution | 0.0001 OD |
Pathlength correction | yes |
Monochromator wavelength accuracy | +2 nm |
Monochromator wavelength repeatability | +0.2 nm |
OD accuracy | <1% at 2.0 OD |
<3% at 3.0 OD | |
OD linearity | <1% from 0 to 3.0 OD |
OD repeatability | <0.5% at 2.0 OD |
Stray light | 0.03% at 230 nm |
Reading speed (kinetic) | 96 well: 6 seconds |
384 well: 11 seconds | |
1536 well: 25 seconds | |
FLUORESCENCE INTENSITY | |
Light source | Xenon flash |
Detector | Dual top PMTs |
Single top PMT (option) | |
Low noise PMT (bottom filter system) | |
Red shifted PMT (top/bottom monochromator system) | |
Wavelength selection | Quad monochromators (top/bottom) |
Filters (top/bottom) | |
Wavelength range | Monochromators: 250 – 850 nm |
Filters (dual PMT): 200 – 850 nm | |
Monochromator bandwidth | Variable, from 3 to 50 nm in 1 nm increments |
Dynamic range | 7 decades |
Sensitivity | Filters: |
Fluorescein 0.2 pM (4 amol/well, 20 µL, 384-well low volume plate) – top | |
Fluorescein 1 pM (10 amol/well, 10 µL, 1536-well plate) – top | |
Fluorescein 1 pM (0.1 fmol/well, 100 µL, 384-well plate) – bottom | |
Quad monochromator: | |
Fluorescein 2 pM (40 amol/well, 20 µL, 384-well low volume plate) – top | |
Fluorescein 2.5 pM (0.25 fmol/well, 100 µL, 384-well plate) – bottom | |
Reading speed (kinetic) | 96 well: 6 seconds |
384 well: 11 seconds | |
1536 well: 25 seconds | |
LUMINESCENCE | |
Wavelength range | 300 – 700 nm |
Dynamic range | > 6 decades |
Sensitivity | 5 amol ATP typical (384-well low volume plate) |
FLUORESCENCE POLARIZATION | |
Light source | Xenon flash |
Detector | Dual PMT or single PMT (option) |
Wavelength selection | Filters |
Wavelength range | 280 – 850 nm |
Sensitivity | 1 mP standard deviation at 1 nM fluorescein |
(384-well low volume plate) | |
1.5 mP standard deviation at 1 nM fluorescein (1536-well plate) | |
TIME-RESOLVED FLUORESCENCE | |
Light source | Xenon flash |
Detector | Dual PMT or single PMT (option) |
Wavelength selection | Quad monochromators (top/bottom) |
Filters (top/bottom) | |
Wavelength range | Monos: 250 – 850 nm |
Filters (dual PMT): 200 – 850 nm | |
Sensitivity | Europium 40 fM (384-well low volume plate) |
Europium 70 fM (1536-well plate) | |
ALPHA DETECTION | |
Light source | 100 mW 680 nm laser |
Detector | PMT |
Wavelength selection | Filters (top) |
Sensitivity | 100 amol LCK peptide (384-well low volume plate) |
Read speed | 96 well: 30 seconds |
384 well: 1 minute 50 seconds | |
1536 well: 7 minutes 20 seconds | |
REAGENT DISPENSERS | |
Supported detection modes | All modes |
Number | 2 syringe pumps |
Supported labware | 6- to 384-well plates, Petri dishes |
Dead volume | 1.1 mL with back flush |
Dispense volume | 5 – 1000 µL in 1 µL increments |
Dispense accuracy | + 1 µL or 2% |
Dispense precision | < 2% at 50-200 µL |
PHYSICAL CHARACTERISTICS | |
Power | 250 Watts max. |
Dimensions | 15.4″ W x 20.7″ D x 16.1″ H |
(39 x 52.5 x 41 cm) | |
Weight | 78 lbs (35 kg) |
REGULATORY | |
Regulatory | CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available. |
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