xCELLigence RTCA eSight

Combining label-free impedance technology with brightfield imaging

Whole well imaging expansion

3 fluorescent channels spanning

Single setup for dual measurements

Information

Combine label-free impedance technology with brightfield imaging

The Agilent xCELLigence RTCA eSight enables comprehensive insight into a cell’s health, behavior, function, and biological processes using live, simultaneous, and real-time biosensor-based and image-based measurements.The eSight system combines label-free impedance technology with brightfield imaging, three fluorescent colors (red, green, and blue), and 5x, 10x, and 20x objectives. Boost your confidence and get more insightful data with ease as you maximize your efficiency with five total readouts.

Combine label-free xCELLigence real time cell analysis technology with live cell imaging in red, green, and blue for increased confidence in your cell analysis and conclusions.
Simple workflow adapts to differing experimental protocols to deliver physiologically relevant data while monitoring cell health, strength of adhesion, changes in morphology, proliferation, and cytolysis in primary cell cultures or standard tissue culture cell lines.
Flexible live cell imaging utilizes brightfield capabilities, three fluorescence channels, a multitude of well plate formats, and the capability of user-defined schedules.

Impedance: Simple workflow, label free, highly sensitive and robust. Very easy automated data output. Ideal for capturing rapid cellular changes.

Imaging: Ideal for suspension cells, single cell analysis, monitoring rare populations, 3D tumor spheroids, and other applications.

Features:

Combine label-free xCELLigence Real-Time Cell Analysis technology with live-cell imaging in red, green, and blue spanning 5, 10, and 20x objectives for increased confidence in your cell analysis and conclusion
Whole well imaging expansion: Visualize the entire well when doing QC or quantifying and monitoring cell growth
Multiplexed and information-rich assays combining cellular impedance and imaging in the same well, all inside your incubator
Deliver physiologically relevant data whether 2D or 3D based cell cultures, with a simple workflow that adapts to differing experimental protocols while monitoring cell health, behavior, and function
Utilize flexible live-cell imaging brightfield capabilities and three fluorescent channels spanning a multitude of microplate forms (6-, 12-, 24-, 48-, 96-, or 384- well) and vessel types (35 or 60 mm dishes), and the capability of user-defined schedules
Analyze a continuous readout of cell number, proliferation size, cell size/shape, changes to morphology, and cell-substrate attachment quality

Analyzer Application	Adhesion	Apoptosis	Cell Characterization
	Cytotoxity	Immune Cell Killing	Proliferation
	Receptor Signaling	Stem Cells	Virus Cytopathic Effects
	Live Cell Imaging
Depth	44.5 cm
Height	41.4 cm
Operating Environment Relative Humidity	5-98 %
Operating Environment Temperature	20-40 °C
Sampling Format	3 x 96-well plate	5 x 96 well plates
Width	43.1 cm

Immune Cell Killing Assays

Typical immune cell killing assays are labor-intensive and require users to collect multiple timepoints using a combination of techniques. In contrast, the label-free xCELLigence RTCA instruments provide a complete view of immune cell killing and closely mimics activity in vivo. Obtain highly sensitive, reproducible, and direct measurements of immune cell killing potency in real time. This simple and high throughput workflow also provides greater insight into cellular mechanism of action, serial killing, and exhaustion.

Viral Cytopathic Effect Assays

Advance your anti-viral therapy or vaccine development by accurately measuring viral cytopathic effects (CPE) in real time with Agilent xCELLigence RTCA instruments, without the use of labor-intensive plaque assays. Monitor viral CPE automatically, from minutes to days, and greatly reduce workload and manual handling of samples. Measure cell proliferation kinetics, identify the optimal time point for viral infection with different cell seeding densities, and assess virus-mediated cytopathogenicity in one workflow.

Cytotoxicity Assays

Determine cytotoxic effects of drugs or compounds by monitoring cell proliferation, cell size or morphology, and cell-substrate attachment quality in real time. Monitor the kinetics of long-term cellular responses with this simple and high-throughput cytotoxicity assay. Make informed decisions about the timing of cell treatments and easily generate dose-response curves at multiple time points, without increasing the workload.

Apoptosis and Proliferation Assays

Learn about apoptosis and proliferation assays that are simple, quantitative, and high throughput to provide multiple perspectives into cell health and viability. Measure real-time cell health directly and capture early and late apoptotic events such as modulation of cell adhesion, cell membrane integrity, and cell detachment. Track proliferation of host cells to detect readout for drug toxicity or presence of a virus. Detect and quantify apoptosis in living cells by label-free approach, or using apoptotic or nuclear labeling reagents. The xCELLigence RTCA eSight provides multiple temporal vantage points (impedance, fluorescence imaging, and brightfield imaging).

Phagocytosis

Dynamically analyze key mechanisms by which the immune system removes pathogens and cell debris. Track the entire process in real-time by imaging, screen for pathway modulators, and discover therapeutic interventions. Label and visualize macrophages via nuclear labeling reagents.

Step 1 Seed cells, then place plates or tissue culture vessels on the eSight in the incubator. Set up data and imaging acquisition schedule using eSight software.

Step 2 Add treatments (e.g. immune effector cells, antibodies, anti-viral drugs)

Step 3 Automatically acquire data with the proprietary xCELLigence biosensor technology and overlay images in real time

Single Setup for Dual Measurements Live cell imaging and real-time biosensor measurements can be performed on the same cell populations, which provides insightful information on cell behavior. The streamlined workflow, high reproducibility, and quantitative kinetics of the eSight system makes it ideal for a wide range of cell-based assays such as proliferation, cytotoxicity, and apoptosis.The powerful RTCA software integrates two data types into one temporal display. Cell analysis can be displayed and exported into diverse formats, which include RTCA images, KT50 (time to reach 50% cytolysis at a given E:T ratio), % Cytolysis dose response, or IC50 dose response curves.

Multi-modal Data Acquisition In concert with the biosensor measurements, cell images can be captured in real-time, thereby providing a spatial and temporally dynamic view of cell populations, which can further validate time-dependent cell health and behavior at an unprecedented level of detail for any cell-based assay.

Patented Real Time Cell Analysis The impedance-based xCELLigence technology utilizes proprietary microplates (E-Plates) embedded with gold biosensors at the bottom of each well, which serve to non-invasively quantify cell behavior. Over the course of an experiment, the biosensors monitor cell metrics such as proliferation, adhesion strength, changes in morphology, migration, differentiation, and much more. The impedance measurements are exceptionally fast and provide exquisite temporal resolution so that all relevant responses can be measured in seconds, minutes, hours, or days.

The eSight offers a unique combination of the features of xCELLigence with live cell imaging. Even more information can be gathered from the live cell analysis when using impedance-based biosensors and image-based measurements together. Both quantitative and qualitative phenotypic information about cell health, behaviour, function and biology processes can be gathered through these combined technologies. The eSight images in 3 colours: red, green, and blue to provide powerful and informative images of cells.

Typical Applications

• Cancer immunotherapy: With the monitoring of cell killing • Virology & Infectious diseases: Monitor pathogens behaviour • Cell signalling: Capture changes in cell behaviour due to signalling • Cytotoxicity Overview: Monitor cell behaviour and attachment to the plate surface • Cell Barrier Function: Continuous monitoring of the cell barrier • Cell Adhesion: Studying cell adhesion and cell spreading

Related instruments

NovoCyte Flow Cytometer Systems

High-performance benchtop flow cytometer, 1-3 lasers

Up to 17 parameter detection

Multiple sampling options

Fixed optical alignment, 24-bit detection dynamic ranges and no need for PMT voltage adjustment

xCELLigence RTCA DP

Make continuous measurements of cell invasion and migration (CIM) using an electronically integrated Boyden chamber

Monitoring of cell invasion and migration in real-time

Non-invasive and label-free real-time impedance assay

FDA 21 CFR Part 11 compliance

xCELLigence RTCA SP

Single plate RTCA Analyzer with uses biosensors to monitor in real-time cell proliferation & viability, biofilm formation & dissipation, and cytophathic effects in a label-free manner

Non-invasive and label-free real-time impedance technology

Simply add cells to E-plates and begin kinetic measurements

Minimal hands-on time

Information

Combine label-free impedance technology with brightfield imaging

Features:

Immune Cell Killing Assays

Viral Cytopathic Effect Assays

Cytotoxicity Assays

Apoptosis and Proliferation Assays

Phagocytosis

Typical Applications

Related instruments

Recently viewed instruments

Newsletter

BioSPX

Our Businesses

The Netherlands

Belgium