BioTek Cytation C10

Confocal and widefield Imaging Reader. Combines spinning disk confocal and automated digital widefield microscopy, plus a multi-mode microplate reader.

Water-immersion objectives to capture more light with lower exposure times

Benchtop confocal imaging for every laboratory

Facilitate live cell imaging with environmental controls

Information

The Cytation C10 confocal imaging reader combines automated confocal and widefield microscopy with conventional multimode microplate reading in a unique, proprietary design. The automated water-immersion objectives capture more light, driving lower exposure times and reducing phototoxic impacts on live cells. The spinning-disk confocal module and accompanying disk pinhole options allow a researcher to look deeper into thick sample biology with improved clarity and detail. High-quality components used in the system, including a Hamamatsu scientific CMOS (sCMOS) camera, Olympus objectives and laser-based illumination enable excellent image quality, at a significantly lower price than others. Cytation C10 also includes widefield fluorescence, brightfield, and phase contrast optics.

Cytation C10 includes widefield fluorescence, brightfield, and phase contrast optics.

Onboard environmental controls enable live cell assays. Multiplate live cell assays are accomplished with Cytation C10 integrated with the Agilent BioTek BioSpa 8 automated incubator. Cytation C10 brings high-performance, affordable confocal to every laboratory.

Cytation C10 offers affordable, benchtop confocal imaging for every laboratory
Confocal microscopy enables improved image quality and analysis, revealing a level of detail in samples that is not possible with widefield optics
High-quality optical components allow the capture of stunning, publication-quality images
Water-immersion objectives to capture more light with lower exposure times
Combination of spinning disk confocal and widefield imaging plus multimode reader, allows the Cytation C10 to be ready for any assay with a modular, upgradable design
An available 60 µm deep sectioning disk (DSD) allows a clear view into thicker samples
Widefield imaging allows for faster acquisition of large samples at lower magnification, while confocal images small intracellular details or 3D samples, and both modes together provide highly multiplexed, multiparameter imaging experiments
Environmental controls, including incubation and CO2/O2 control and monitoring, facilitate live cell imaging
Imaging plus multimode detection and the hit-picking function quickly prescreen the microplate with the plate reader optics, then automatically image the samples that meet your hit criteria, saving time and data storage capacity
Variable bandwidth, quad monochromator plate reader optics enhance assay performance, including increased sensitivity and specificity
Combination of confocal and widefield imaging with multimode detection increases productivity with the ability to be ready for any application

Variety of applications from endpoint measurements to live cell imaging:

Cell viability/toxicity assays
3D Cell Culture Assays
Cell Migration and Invasion Assays
Cell Proliferation
Label-free cell counting for cell proliferation studies
Cell Metabolism imaging and normalization – Seahorse data
Genotoxicity
Plaque assay
Fluorescent Microneutralization Assay

Configurations

Part #	C10PHC2	C10MPHC2	C10PWC	C10MPWC	C10PW	C10MPW
Confocal 60 µm spinning disk	•	•	•	•
Confocal 40 µm spinning disk	•	•
Hamamatsu sCMOS camera	•	•
Sony CMOS camera			•	•	•	•
Multi-mode reading		•		•		•
Phase contrast	•	•	•	•	•	•

Confocal: Improved image quality and analysis

Confocal microscopy can enable you to see a level of detail in your samples that is not possible with widefield optics. Not only can you obtain improved image quality, you can also get improved quantification and analysis with confocal images and Gen5 software.

GENERAL
Detection modes	UV-Vis absorbance
	Fluorescence intensity
	Luminescence
Read methods	Endpoint, kinetic, spectral scanning, well area scanning
Microplate types	Monochromator: 6- to 384-well plates
Microplate types	Imaging: 6- to 1536-well plates
Other labware supported	Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer), Take3 Micro-volume plates
Temperature control	4-Zone incubation to 45 °C with Condensation Control
Shaking	Linear, orbital, double-orbital
Software	Gen5 Microplate Reader and Imager Software included
	Gen5 Secure for 21 CFR Part 11 compliance (option)
	Gen5 Image+ and Image Prime software available for full image analysis (option)
Automation	BioStack and 3rd party automation compatible
CO2 and O2 control (option)	Range: 0 – 20% (CO2); 1 – 19% (O2), with optional Gas Controller
CO2 and O2 control (option)	Models for both CO2 and O2 or CO2 only are available
IMAGING – CONFOCAL MICROSCOPE
Imaging modes	Fluorescence
Image processing	Z-projection, digital phase contrast, stitching
Camera	Hamamatsu Orca sCMOS, 16-bit grayscale camera or
Camera	Sony CMOS 16-bit grayscale camera
Objective capacity	6-position automated turret for user-replaceable objectives
Objectives available	20x, 40x, 60x
Image filter cube capacity	4 user-replaceable fluorescence cubes
Imaging filter cubes available	CFP, CY5, DAPI, GFP, RFP, TRITC, brightfield
Laser	6-line
Automated functions	Autofocus, user-trained autofocus, autoexposure, auto-LED intensity
Autofocus method	Image-based autofocus
	User-trained autofocus
	Laser autofocus (option)
Positional controls	Software Control
Positional controls	Joystick controller (option)
Imaging methods	Single color, multi-color, time lapse, montage, z-stacking, z-stack montage
Field of view	Hamamatsu sCMOS: 0.65 mm + 5% at 20x magnification
Field of view	Sony CMOS: 0.70 mm + 5% at 20x magnification
Image collection rate	Laser autofocus, 0 ms delay, 96 wells: 8 mins, 9 secs
Image collection rate	Software autofocus, 0 ms delay, 96 wells: 12 mins, 1 sec
IMAGING – WIDEFIELD MICROSCOPE
Imaging mode	Fluorescence, phase contrast, color brightfield, user-selectable brightfield/high contrast brightfield
Imaging method	Single color, multi-color, time lapse, montage, z-stacking, z-stack montage
Image processing	Z-projection, digital phase contrast, stitching
Camera	Hamamatsu Orca sCMOS, 16-bit grayscale camera or
Camera	Sony CMOS 16-bit grayscale camera
Objective capacity	6-position automated turret for user-replaceable objectives
Objectives available	1.25x, 2.5x (2.25x eff), 4x,10x, 20x, 40x, 60x
Phase objectives available	4x, 10x, 20x, 40x
Image filter cube capacity	4 user-replaceable fluorescence cubes, plus brightfield channel
Imaging filter cubes available	DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-FRET, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, Tag BFP-FRET, GFP (Ex)-CY5 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex 377/Em 647, Oxidized roGFP2, TRITC
Imaging LED cubes available	365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 554 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions	Autofocus, autoexposure, auto-LED intensity
Autofocus method	Image-based autofocus
	User-trained autofocus
	Laser autofocus (option)
Positional controls	Software Control
Positional controls	Joystick controller (option)
Image collection rate	Image-based autofocus:
	96 wells, 1 color (DAPI), 4x, 6 minutes
	Laser autofocus:
	96 wells, 1 color (DAPI), 4x, <3 minutes
Image Analysis Software option	Gen5 Image+: Image analysis
	Gen5 Image Prime: Advanced image analysis
	Gen5 Secure: 21 CFR Part 11 compliant features
FLUORESCENCE INTENSITY
Light source	Xenon flash
Detector	PMT
Wavelength selection	Quad monochromators (top/bottom)
Wavelength range	250 – 700 nm (900 nm option)
Monochromator bandwidth	Variable, from 9 nm to 50 nm in 1 nm increments
Dynamic range	7 decades
Reading speed (kinetic)	96 wells, sweep mode: 10 seconds
Reading speed (kinetic)	384 wells, sweep mode: 20 second
LUMINESCENCE
Wavelength range	300 – 700 nm
Dynamic range	>6 decades
ABSORBANCE
Light source	Xenon flash
Detector	Photodiode
Wavelength selection	Monochromator
Wavelength range	230 – 999 nm, 1 nm increments
Monochromator bandwidth	4 nm (230 – 285 nm), 8 nm (>285 nm)
Dynamic range	0 – 4.0 OD
Resolution	0.0001 OD
Pathlength correction	Yes
Monochromator wavelength accuracy	+ 2 nm
Monochromator wavelength repeatability	+ 0.2 nm
OD accuracy	<1% at 3.0 OD
OD linearity	<1% from 0 to 3.0 OD
OD repeatability	<0.5% at 2.0 OD
Stray light	0.03% at 230 nm
Reading speed (kinetic)	96 wells: 10 seconds
Reading speed (kinetic)	384 wells: 20 seconds
REAGENT INJECTORS (OPTION)
Supported detection modes	All modes
Number	2 syringe pumps
Supported labware	6- to 384-well plates, Petri and cell culture dishes
Dead volume	1.1 mL, with back flush
Dispense volume	5 – 1000 µL in 1 µL increments
Plate geometry	6- to 384-well microplates
Dispense accuracy	+1 µL or 2%
Dispense precision	< 2% at 50 – 200 µL
PHYSICAL CHARACTERISTICS
Power	Instrument: External 250W (minimum), 24VDC power supply, compatible with 100-240 VAC @50-60Hz.
	Optional 6-channel laser light source: External 250W power supply, compatible with 100- 240VAC @ 50-60Hz.
	Optional Hamamatsu scientific camera: External 75W power supply, compatible with 100- 240VAC @ 50-60Hz.
Dimensions	27″ W x 18.5″ H x 20″ D
Dimensions	(68.6 cm x 45.72 cm x 50.8 cm)
Weight	122 lbs (53.3 Kg)
REGULATORY
Regulatory	CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.

Gas Controller for CO2 and O2 controll Increasingly, life science assays are run on live cells. Some of these assays require control over CO2 and O2 concentrations to modulate the environment for pH buffering or to create a hypoxic condition. These assays are traditionally run-in flasks in a low-throughput manner, but there is a burgeoning interest in automation of live cell-based assays in microplates for higher throughput and efficiency, therefore there is a need for cell-friendly microplate instrumentation. The Gas Controller allows full control over CO2 and O2 concentrations to regulate the environment for microplate based live cell assays.

From whole organism imaging to high magnification subcellular image analysis, Gen5 helps transform raw data and images into meaningful results. Gen5 Software automates image capture from Agilent’s Lionheart Automated Microscope and Cytation Cell Imaging Multi-Mode Reader, and automates image processing and analysis to produce publication-ready images and quantitative data. This process of Augmented Microscopy™ enables simple, straightforward imaging workflows. • Automated image capture • Exceptional image processing tools • Powerful image analysis • Publication-ready images, graphs and data

Features

Augmented Microscopy automates image capture for samples with powerful tools for endpoint and time lapse workflows. Fluorescence, brightfield, color brightfield and phase contrast images are captured in slides, microplates, chamber slides, cell culture dishes for quantitative and qualitative analysis.

Exceptional Image Processing Tools Gen5’s image processing tools provide exceptional processing capability to facilitate the analysis of complex biologies, including 3D samples, large montaged samples and processes including live cell kinetics. From easy brightness/ contrast adjustments to deconvolution and automated movie file creation, image processing in Gen5 enables optimized visualization and analysis for many applications. The independent optical paths ensure uncompromised performance in all modes

Powerful Image Analysis Image analysis tools in Gen5 cover a very broad range of application requirements, and are both powerful and easy to use. Analysis functions in Gen5 extend to quantitative data as well. Cell count, confluence, signal translocation, subpopulation analysis and kinetic analysis are just some of the powerful data provided by Gen5’s image analysis tools.

Publication-ready Images, Graphs and Data Augmented Microscopy tools include the ability to create publication-ready images, graphs and data using the functions in Gen5 software. There is no need to export images or data to external software to create scatterplots, histograms, EC50 graphs and other data displays for publication.

Combined confocal and widefield microscopy Image quality is considerably improved due to the combination of spinning disk confocal and widefield microscopy. The degree of detail is exceptionally enhanced, and this results in improved quantification and analysis of the assay, combined with the Gen5 imaging Software. High quality optical components To enable high quality images and publication-ready images, high standard objectives and filters are compatible with the Cytation C10. These high quality objectives and filters include the Hamamatsu sCMOS Orca, Semrock filters, Olympus objectives and other known brands. Hit-picking: Multi-mode detection + imaging saves time and data storage The plate reader screens all wells and identifies the wells that meet certain criteria. Only the wells that meet these, will be imaged by the microscope. This results in the saving of time and computer memory! (1) Plate reader quickly identifies GFP positive wells. (2) Only GFP positive wells are imaged, saving both time and computer memory.Variable bandwidth monochromator for sensitivity and specificity The bandwidths of the monochromators in the Cytation C10 fluorescence detection module is selectable from 9 nm to 50 nm, in 1 nm increments. Variable bandwidths allow you to optimize detection conditions for fluorophores that have unique excitation or emission parameters, for example, a narrow Stokes shift. Adjusting the bandwidth results in better performance than conventional monochromators. The plate reader optics of Cytation use a quad monochromator design with variable bandwidth. The bandwidth can be set anywhere between 9 and 50 nm in 1 nm increment. Large bandwidth settings (1) provide increased sensitivity and lower limits of detection. Small bandwidth settings (2) provide increased specificity when multiple signals are present, which reduces signal crosstalk and enhances assay performanceMaintaining a consistent environment through CO2/O2 and temperature control Cytation C10 is well suited for live cell assays, with temperature control to 45 °C, plus linear and orbital shaking. A gas controller module is available to help regulate CO2 and O2 levels in the system for longer-term live cell requirements. A dual reagent injector provides quick inject/image capability for observing fast reactions. Successful live cell kinetic imaging relies on a consistent environment, including temperature control and CO2/O2 control and monitoring. Cytation C10 provides the perfect environment to grow and analyze live cells over time. Powerful movie maker and kinetic analysis software tools allow visualizing and analysis time-lapse experiments.Label-free cell counting It’s fast and convenient to count live cells using the available high contrast brightfield option. Higher contrast allows a direct and label-free method for object identification using Gen5 software to count cells and characterize cell proliferation in kinetic studies.

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Information

Cytation C10 includes widefield fluorescence, brightfield, and phase contrast optics.

Variety of applications from endpoint measurements to live cell imaging:

Confocal: Improved image quality and analysis

Features

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