PopBio VüF for Fluorescence

Clever working as standard

Overview

Unlike a traditional CCD based gel documentation system the Vü works just like a ‘copier’ for gels and blots. The user just has to define the illumination type, size of gel and optional User ID using three thumbwheels. Once selected the Vü processors take over and determine how best to capture an image. A fluorescence gel will be mapped in around 1 minute.

Vü-F has a clever sensor array which is placed very close to the sample in order to collect the light output. The array moves across the sample to map the output and to produce an image – amazingly it can detect the faintest of signals. Images are then sent via a network connection or to a computer which directly connects to the Vü for on bench viewing.

PopBio Vü Details



Vü-F Vü-C Vü-CF
Application Fluorescent gels and blots Chemiluminescence Western blots Fluorescent gels and blots Chemiluminescence Western blots
Illumination UV integral 302nm (1 tube) Blue light integral LEDs
White light conversion screen
White LED’s for marker lane
RGB LED’s for coloured marker lane
UV integral 302nm (1 tube) Blue light integral LEDs
White light conversion screen White LED’s for marker lane
RGB LED’s for coloured marker lane
Sample size (cm) 20 x 20 10 x 10 20 x 20 (F) and 10 x 10 (C)
Sample Drawer Thumbwheel selection for lighting, ROI and user Thumbwheel selection for lighting and user Thumbwheel selection for lighting, ROI (F only) and user
Drawer type Pull out drawer with removable washable gel tray Pull out drawer with removable washable blot tray Pull out drawer with removable washable gel tray
Sensor resolution 90M pixel 51M pixel 90M pixel & 51M pixel
Image output resolution 3550 x 3550 (12.6M pixel) 1530 x 1530 (2.34M pixel) 3550 x 3550 (12.6M pixel) (F)
1530 x 1530 (2.34M pixel) (C)
Image format TIFF TIFF TIFF
Sensor type CMOS linear CMOS linear high sensitivity CMOS linear high sensitivity
Process speed 20-90 seconds - depends on gel and dye used 2-5 minutes - depends on blot and reagent 20-90 seconds - depends on gel and dye used (F)
2-5 minutes - depends on blot and reagent (C)
Footprint (mm) 480D x 426W x 205H 375D x 353W x 175H 480D x 426W x 205H (F)
375D x 353W x 175H (C)
Power Supply 100-240V external 100-240V external 100-240V external
Connectivity Integral ethernet; optional external WiFi Integral ethernet; optional external WiFi Integral ethernet; optional external WiFi
Analysis software 3 copies 3 copies 3 copies
Warranty 2 years 2 years 2 years

The latest CMSO Technology
Instead of a camera, the Vü’s head unit contains a linear array of high sensitivity CMOS sensors; moved in close proximity across the sample, backwards and forwards multiple times to collect the light output. The position and output level of the light emission is measured, with increases or decreases recorded. Once the Vü’s head unit has collected enough light to form an image (just 60 seconds for fluorescence and 2-5 minutes for chemiluminescence wester blot), the device automatically sends it to a device for viewing – thanks to a fully automatic imaging process controlled by the embedded intelligent software.

The CMOS Advantage
It is well known that CMOS sensors outperform CCD’s in most applications such as in fluorescence and chemiluminescence where there is a visible light output. Also a very important feature of a CMOS sensor is the incredible low noise as compared to a CCD. Of course CMOS technology has advanced significantly in the last few years ( just take a look at your mobile phone which uses the same technology) and that is why in the Vu systems we are using the very latest variants to produce high end systems at significantly lower prices than conventional CCD based units. CCD systems are really a throw back to the past – it’s time for change.

Super high resolution as standard
The Vü-F can image samples using UV, blue light or white light. The sensor set up uses an amazing 90m pixels of resolution to collect a high end image. Images are saved as 16bit TIFF or can be printed directly from the interface. To make the images easier to handle we output them to a more manageable 12m pixels at 300dpi – still more than enough to make large poster size prints with no loss of detail or data.

Imaging Superiority over CCD
When it comes to chemiluminescence the Vü-C really does excel. With over 51m pixels of resolution then you can expect high end images. Again to make them more manageable we output images at 2.34m pixels which can still produce a high quality poster image. Not only does the Vü-C image the blot itself it can also be set to image coloured or black/white markers. All of this is setup using the simple thumbwheel selector system found in the Vü range. Crucially, because of the high performance CMOS sensors the system produces images with virtually zero background without the need for any expensive Peltier cooling which is necessary on a CCD based system.

The Vü software is used to collect, save and print images - and it also provides access to the system set up menus. The Vü processor creates the image and sends it to a network via and ethernet or WIFi connection. Alternatively, Vü can be connected to a stand-alone computer.

The Vü software is compatible with any computer running Windows 7 or higher. The Vü software also has hidden setup functions that can be accessed by a qualified person to be able to make further adjustments to the operation of the Vü system.


Analysis software
Each Vü system is supplied with a copy of 1D analysis software
• Analysis of 1D gels and Western blots is rapid and automated to a high level and reproducible.
• Highly developed algorithms accurately detect lanes and bands even on distorted gel images.
• Bands calibrated using one or more Molecular Size standard lanes.
• Absolute band quantitation derived using known quantity calibration standards in samples.
• Ability to review each stage of workflow analysis and intervene / edit if required.
• High levels of automation combined with final user review for rapid and accurate quantitative analysis.