Immune cell-mediated tumor cell killing can involve the components of both the innate and adaptive immune systems, including natural killer (NK) cells, cytotoxic T cells (MHC-dependent), antibodies secreted by B lymphocytes, engineered antibodies such as bispecific antibodies and bispecific T cell engagers (BiTEs), and genetically engineered T cells targeting specific tumor antigens (e.g., CAR-T, MHC-independent). The xCELLigence Real-Time Cell Analysis (RTCA) instrument monitors cell killing in real time. Simply add target and immune effector cells to the patented microplates (E-Plates), load the instrument, and start reading.
Virology & Infectious Diseases
Study pathogen fitness and behavior in real-time by using xCELLigence Real-Time Cell Analysis (RTCA) instruments. Continuously monitor viral infections and bacterial biofilms in an automated, non-invasive, and label-free manner.
Cell Invasion & Migration
Cell invasion and migration is critical to many facets of biology, including processes such as wound healing and the intravasation/extravasation of cancer cells during metastasis. A major hurdle to studying migration and invasion is the lack of techniques that are quantitative, reproducible, and efficient. Agilent's cell invasion and migration platform (CIM-Plate used with the xCELLigence RTCA DP system) contains biosensor integrated Boyden chambers that provide real-time, label-free continuous monitoring of cell migration/invasion with minimal reqired setup.
xCELLigence real time cell analysis (RTCA) uses biosensors on the bottom of E-Plate wells to continuously monitior the number of cells present, their size/morphology, and how tightly they are interacting with the plate surface. Cytotoxic responses nearly always involve biochemical changes that directly, or indirectly, affect one or more of the these parameters. Consequently, xCELLigence is able to monitor cytotoxic responses resulting from an exceptionally wide range of molecular targets (Figure 1).
Stem Cell Differentiation
xCELLigence Real-Time Cell Analysis captures the process of differentiating stem cells into somatic cells. The kinetics of changes in real time and provide quantitative assessment of cell morphology, membrane potential, metabolic activity, and responsiveness to certain signals.
xCELLigence Real-Time Cell Analysis (RTCA) instrument continuously monitor cell adhesion and spreading without any manipulation of cells. Study interactions between extracellular matrix (ECM) proteins and cell surface integrins in an automated, non-invasive, and label-free manner.