Immune cell-mediated tumor cell killing can involve the components of both the innate and adaptive immune systems, including natural killer (NK) cells, cytotoxic T cells (MHC-dependent), antibodies secreted by B lymphocytes, engineered antibodies such as bispecific antibodies and bispecific T cell engagers (BiTEs), and genetically engineered T cells targeting specific tumor antigens (e.g., CAR-T, MHC-independent). The xCELLigence Real-Time Cell Analysis (RTCA) instrument monitors cell killing in real time. Simply add target and immune effector cells to the patented microplates (E-Plates), load the instrument, and start reading.
Virology & Infectious Diseases
Study pathogen fitness and behavior in real-time by using xCELLigence Real-Time Cell Analysis (RTCA) instruments. Continuously monitor viral infections and bacterial biofilms in an automated, non-invasive, and label-free manner.
xCELLigence Real Time Cell Analysis captures changes in cell size, shape, and proliferation rate in response to membrane receptor signaling. Label-free and continuous monitoring provides insights in cell response in the initial minutes of exposure and their response over days.
xCELLigence real time cell analysis (RTCA) uses biosensors on the bottom of E-Plate wells to continuously monitior the number of cells present, their size/morphology, and how tightly they are interacting with the plate surface. Cytotoxic responses nearly always involve biochemical changes that directly, or indirectly, affect one or more of the these parameters. Consequently, xCELLigence is able to monitor cytotoxic responses resulting from an exceptionally wide range of molecular targets (Figure 1).
Cell Barrier Function
The impedance-based xCELLigence Real Time Cell Analysis (RTCA) assay provides a label-free automatic alternative to solute permeability assay and transendothelial electrical resistance (TEER) assay. RTCA assays provides continuous monitoring of cell barrier functions in response to disease state, inflammation, and compound treatment.
xCELLigence Real-Time Cell Analysis (RTCA) instrument continuously monitor cell adhesion and spreading without any manipulation of cells. Study interactions between extracellular matrix (ECM) proteins and cell surface integrins in an automated, non-invasive, and label-free manner.